Oxytocin (OT) action in uterine tissues of cyclic and early pregnant gilts: OT receptors concentration, prostaglandin F2α secretion, and phosphoinositide hydrolysis

Franczak, Anita; Ciereszko, Renata E.; Kotwica, Genowefa

doi:10.1016/j.anireprosci.2004.12.011

Cited by 30 publications

(35 citation statements)

References 37 publications

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“…Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][23][24][25].…”

mentioning

confidence: 99%

“…55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].…”

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confidence: 99%

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Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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Abstract. In the pig, the periimplantation period is critical for successful establishment of pregnancy. We studied the influence of embryos on oxytocin (OT) and progesterone (P4) regulated endometrial and myometrial secretion of 1) luteotrophic prostaglandin E2 (PGE2) and 2) luteolytic prostaglandin F2α and its metabolite (PGFM) on days 12-14 of pregnancy in pigs. We used unilaterally pregnant pigs created by a surgical procedure in which one uterine horn remained intact and the second horn was cut transversely so that part of the horn was detached from the uterine body. The animals were divided into two groups, inseminated gilts (days 12-14 of pregnancy, n=6) and uninseminated cyclic gilts, which were used as controls (days 12-14 of estrous cycle, n=5). Embryos developed only in the patent part of the uterus and not in the occluded horn. The abundance of OTR mRNA was increased in the endometrium and decreased in the myometrium of the gravid uterine horn in the pregnant pigs compared with the non-gravid uterine horn or either uterine horn in the cyclic pigs, indicative of a local effect of the conceptus. The presence of embryos in the uterine horn during the periimplantation period determines endometrial metabolism of PGF2α and the local response of the endometrium to OT and P4. OT stimulates PGF2α secretion and PGFM accumulation in endometrial cultures only from the non-gravid uterine horn and controls PGE2 secretion from the endometrium and myometrium in the pregnant gilts. The results indicate a more systemic affect of pregnancy on the uterine response to OT and a possibly the local effect of the conceptus in promoting progesterone's inhibition of OT-stimulated PGE2 secretion and uterine metabolism of PGF2α. Key words: Embryos, Oxytocin, Pigs, Progesterone, Prostaglandin E2, Prostaglandin F2α and its metabolite, Uterus (J. Reprod. Dev. 55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][2...

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confidence: 99%

mentioning

confidence: 99%

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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“…Nevertheless, a high basal PI hydrolysis observed in the early CL might be responsible for the absence of the stimulatory effect of OT. Similarly, endometrial cells with high basal accumulation of IPs were unresponsive to OT in pigs Uzumcu et al, 1998;Franczak et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In most target cells, OT, after binding to its receptors, activates the intracellular phosphatidylinositol pathway, generating two second messengers: inositol triphosphate (IP) which increases intracellular Ca 2+ ([Ca 2+ ] i ) mobilisation and diacylglycerol (DAG) which activates protein kinase C (PKC) Franczak et al, 2005;Franczak et al, 2006). An association between activation of the PKC- [Ca 2+ ] i system and luteal secretion of P 4 was observed in pigs (Ciereszko et al, 1998) and ruminants (Niswender, 2002).…”

Section: (Received 2 January 2008; Accepted 22 May 2008)mentioning

confidence: 99%

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Franczak

Kurowicka

Kowalik

et al. 2009

Acta Veterinaria Hungarica

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Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3-5), mid-(days 8-10) and late luteal phases (days 12-14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3-5 and 8-10 of the oestrous cycle than on days 12-14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 -7 M) enhanced P 4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8-10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3-5 and 8-10) or 30th second (days 12-14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

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“…W bioptatach z macicy krów wykazano, że w terminie matczynego rozpoznawania ciąży (uwarunkowanego wydzielaniem przez zarodek interferonu tau) dochodzi do zablokowania ekspresji OTR w odróżnieniu od zwierząt niebędą-cych w ciąży, u których w tym czasie jest obserwowany wzrost (34). Z kolei u świń we wczesnej ciąży (14.-16. dzień) nie wykazano OTR w endometrium, podczas gdy w myometrium były one jeszcze obecne w stężeniu podobnym do adekwatnego dnia cyklu rujowego (14).…”

Section: Przyczyny Przedwczesnych Porodówunclassified

Methods of prolonging a pregnancy threatened with preterm labour

Dobrzynski¹,

Max²,

Jurka³

2016

Medycyna Weterynaryjna

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479 Artykuł przeglądowy ReviewWłaściwa, gatunkowo swoista długość ciąży warunkuje odpowiedni rozwój płodów, dojrzałość ło-żyska i fizjologiczny przebieg porodu oraz okresu poporodowego. Odchylenia poza wartości uznane za prawidłowe, zarówno w górę, jak i w dół, stanowią zagrożenie, zwłaszcza dla płodu, zmniejszając szansę jego przeżycia, szczególnie gdy dochodzi do porodu przedwczesnego. Z danych epidemiologicznych wynika, że u ludzi ponad 10% porodów odbywa się przedterminowo, a to oznacza, że rocznie blisko 15 milionów dzieci rodzi się jako wcześniaki, co bezpośrednio skutkuje śmiercią około 1 miliona spośród tych noworodków (3). Długość ciąży jest modulowana przy udziale czynników społecznych i środowiskowych, ale także podlega epigenetycznej regulacji aktywności genów. Stwierdzono na przykład, że zjawisko przedwczesnych porodów jest związane ze zmianami w metylacji DNA i w konsekwencji w ekspresji genów kodujących białka biorące udział w aktywności skurczowej macicy (31). Problemy z niefizjologiczną długością ciąży występują także u zwierząt, w tym psów i kotów. Uważa się, że u tych gatunków płody uzyskują dojrzałość w wieku 56-58 dni. Zatem za poród przedwczesny należy uznać ten, który rozpoczyna się przed tym terminem. Problemem praktycznym jest ustalenie aktualnego wieku płodów, zwłaszcza u psów, o ile wcześniej (w okresie krycia) nie przeprowadzano żadnych badań, które bezpośrednio lub pośrednio wskazywałyby termin owulacji. Jeżeli jest on znany, to przyjmuje się, że po kolejnych 2 dniach może dojść do zapłodnienia, gdy plemniki zostały zdeponowane wcześniej. O ile krycie/unasiennianie odbywa się później niż 2 dni po owulacji, to z początkiem ciąży można się liczyć od następnego dnia. Jeżeli natomiast termin owulacji nie jest znany, to istnieją możliwości określenia zaawansowania ciąży przy wykorzystaniu diagnostyki obrazowej -na podstawie ultrasonograficznego obrazowania struktur zarodkowo-płodowych pomiarów wewnętrznej średnicy jamy kosmówkowej lub podłużnych i poprzecznych pomiarów płodów (fe- Methods of prolonging a pregnancy threatened with preterm labour Summary Both prolonged and shortened pregnancies carry the risk of increased mortality of the offspring. The main causes of early delivery are genetic factors, bacterial and viral infections, corpus luteum insufficiency and pathologies of the placenta. Antepartum luteolysis is due to the activity of prostaglandin F2α. A decrease in the progesterone concentration activates the sensitivity of the uterine muscles to oxytocin. Calcium ions move into the myocytes and bind to calmodulin, starting enzymatic reactions necessary for muscle contraction. Oxytocin promotes prostaglandin F2α release. Cholesterol and magnesium stimulate the binding of oxytocin to its receptors. A premature delivery is caused by a disorder of the balance between contractility and relaxation of the myometrium, which is regulated by many signalling pathways. Birth in humans can be postponed by the use of many different tocolytic medications, β-adrenergic drugs (ritodrine, hexoprena...

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Oxytocin (OT) action in uterine tissues of cyclic and early pregnant gilts: OT receptors concentration, prostaglandin F2α secretion, and phosphoinositide hydrolysis

Cited by 30 publications

References 37 publications

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Methods of prolonging a pregnancy threatened with preterm labour

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