Ozone-induced lipid peroxidation and membrane leakage in isolated rat alveolar macrophages: protective effects of taurine

Banks, Melanie A.; Porter, Dale W.; Martin, William G.; Castranova, Vincent

doi:10.1016/0955-2863(91)90072-d

Cited by 39 publications

(15 citation statements)

References 25 publications

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“…The lungs are very susceptible to free radical injury due to their exposure to high oxygen tension and toxic gases, such as ozone and the nitrogen oxides. According to the work of Banks et al 20 nearly 40% of alveolar macrophages exposed to 0.45 ppm of ozone for 30 min in the absence of taurine were found to lose viability. However, pretreatment of the cells with taurine reduced the extent of ozone-mediated cell death to about 15%, an effect associated with a dramatic reduction in specific measures of oxidative stress, such as lipid peroxidation and reduced glutathione loss.…”

Section: Anti-oxidant and Membrane Stabilizing Activity Of Taurinementioning

confidence: 98%

Why Is Taurine Cytoprotective?

Schaffer

Azuma

Takahashi

et al. 2003

Advances in Experimental Medicine and Biology

137

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Section: Anti-oxidant and Membrane Stabilizing Activity Of Taurinementioning

confidence: 98%

Why Is Taurine Cytoprotective?

Schaffer

Azuma

Takahashi

et al. 2003

Advances in Experimental Medicine and Biology

137

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“…Oxidant exposure to the lung has been shown to cause pulmonary injury which includes destruction of endothelial cells, edema, changes in the bronchiolar epithelium, and an influx of leukocytes into the alveolar spaces [9-141. Banks et al [15] found that preincubation of alveolar macrophages with taurine protected the lung, based on exclusion of trypan blue, decreased lipid peroxidation, and decreased leakage of glutathione. Recently, Wang et al [16] reported that taurine or taurine-niacin combination reduced lung fibrosis and phospholipid accumulation in amiodarone-treated hamsters.…”

mentioning

confidence: 98%

Taurine Protects rat Bronchioles from Acute Ozone Exposure: A Freeze Fracture and Electron Microscopic Study

Gordon¹,

Park²,

Laskin³

et al. 1998

Experimental Lung Research

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Dietary taurine has been shown to protect rat and hamster lung epithelia from acute oxidant injury. One of the earliest morphologic criteria of oxidant injury is the alteration of tight junctions of the peripheral lung airways. In the present study, we have used this criteria to evaluate whether taurine was capable of protecting rat lungs from ozone exposure. Rats were treated for 10 days with 50% taurine in their drinking water, prior to exposure to 2 ppm of ozone for 3 hours. The lungs from rats pretreated with taurine and exposed to ozone were compared to untreated rats exposed to ozone and air-exposed controls. At 2, 6, 12, 24, 48, and 72 hours after exposure to air or ozone, rats were anesthetized and the lungs perfusion-fixed through the right side of the heart with a solution of glutaraldehyde and paraformaldehyde. Light microscopy revealed the typical, mild inflammatory cell infiltrate beginning at 6 hours after ozone exposure in bronchioles, alveolar ducts, and surrounding alveoli which was absent in the lungs of animals treated with taurine. Electron microscopic analysis of thin sections indicated alterations in tight junctions which was confirmed by tracer studies using ruthenium red and lanthanum. Alterations in airway epithelium tight junctions were seen 2 and 6 hours after ozone treatment and only in the 2-hour tissues from animals pretreated with taurine prior to ozone exposure. Freeze-fracture replicas from all exposure groups by electron microscopy revealed that only the 2- and 6-hour groups showed alterations in tight junctions. The alterations were characterized by decreased number of fibrils and breaks in the fibrils. Rats treated with taurine and exposed to ozone exhibited these alterations focally at 2 hours exposure and no changes were noted at 6 hours post ozone exposure. These data confirmed previous findings that injury induced by ozone is transient and that taurine protects the bronchioles from this form of oxidant injury.

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“…In this regard taurine has been used to protect animals from pulmonary fibrosis induced by the oxidant moieties, i.e., superoxide anion and free radicals, which increase in lung tissue after exposure to paraquat (Gordon et al, 1992), amiodarone (Kennedy et al, 1988), bleomycin (Wang et al, 1989), nitrogen dioxide (Gordon et al, 1986(Gordon et al, , 1992, and ozone (Banks et al, 1990(Banks et al, , 1991(Banks et al, , 1992. Gordon and colleagues (1992) reported that when Golden Syrian hamsters were injected with paraquat or given an intratracheal instillation of bleomycin it could be demonstrated by light microscopy (after glutaraldehyde-cacodylate fixation of the lungs) at one day after treatment, a localized, mild edema, and aggregation of large numbers of inflammatory cells in the lung tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Banks and colleagues (Banks et al, 1990(Banks et al, , 1991(Banks et al, , 1992 used isolated alveolar macrophages to study the protective effects of taurine against ozone exposure. In their studies isolated alveolar macrophages were preloaded with taurine (varied concentrations up to 500ttM) prior to ozone exposure.…”

Section: Discussionmentioning

confidence: 99%

“…Paraquat, a broad spectrum herbicide, bleomycin an antineoplastic agent, and amiodarone, an antiarrhythmic agent, produce pulmonary fibrosis possibly by forming superoxides and free radicals in the lung (Hussain et al 1985;Kennedy et al, 1988;Wang et al, 1989;Gordon et al, 1992). Other studies involving taurine as a protecting agent against oxidant injury induced by nitrogen dioxide and ozone in lung tissue have also been reported (Gordon et al, 1986;Banks et al, 1990Banks et al, , 1991Banks et al, , 1992.…”

Section: Introductionmentioning

confidence: 88%

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Effects ofin vivo taurine depletion on induced- chemiluminescence production in macrophages isolated from rat lungs

Zhang

Lombardini

1998

Amino Acids

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Alveolar macrophages isolated by pulmonary lavage from partially taurine-depleted rats demonstrated increased (2-2.6 fold) chemiluminescence due to the extracellular reaction between exogenous zymosan and various reactive forms of oxygen compared to macrophages isolated from control animals. Partial taurine depletion was achieved by adding 3% beta-alanine to the drinking water of the rats for 5 weeks prior to harvesting the macrophages. Superoxide dismutase activity was not increased in the lung tissue of the taurine-depleted rats. These data suggest that taurine has antioxidant properties and that taurine depletion is potentially deleterious to alveolar macrophages and pulmonary tissue.

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Ozone-induced lipid peroxidation and membrane leakage in isolated rat alveolar macrophages: protective effects of taurine

Cited by 39 publications

References 25 publications

Why Is Taurine Cytoprotective?

Why Is Taurine Cytoprotective?

Taurine Protects rat Bronchioles from Acute Ozone Exposure: A Freeze Fracture and Electron Microscopic Study

Effects ofin vivo taurine depletion on induced- chemiluminescence production in macrophages isolated from rat lungs

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