Ozone Therapy Alleviates Monosodium Urate Induced Acute Gouty Arthritis in Rats Through Inhibition of NLRP3 Inflammasome

Abstract

Background: Gout is a metabolic disease strictly related to hyperuricemia. The associated intense inflammation and pain are triggered by the deposited monosodium urate crystals (MSU) in joints. The principal therapeutic strategies of gout involve the control of hyperuricemia and anti-inflammatory medications. Objectives: This study aimed to investigate the possible beneficial effects of ozone therapy, a well-known antioxidant, and an immunomodulation, on gouty arthritis and the underlying mechanisms. Methods : Acute gouty arthritis was induced in male albino rats via MSU crystals intra-articular injection in the ankle joint. The gouty arthritic rats received pre-treatment with ozone, colchicine (as a reference drug), or combination. Results : The obtained results of ozone therapy showed obvious reduction in the degree of ankle edematous swelling, pro-inflammatory cytokines, lipid peroxidation, the nucleotide binding oligomerization domain like receptor containing pyrin domain 3 (NLRP3), procaspase-1, caspase-1, interleukin-1β synovial tissue levels with enhancement of antioxidant defense system. Additionally, ozone therapy significantly attenuated the histological derangements in gouty arthritic rats. Conclusion : This study suggests that ozone is able to treat gouty arthritis and reducing synovial injury through an anti-inflammatory effect as well as antioxidant activity.

Oxygen-Ozone Therapy for Reducing Pro-Inflammatory Cytokines Serum Levels in Musculoskeletal and Temporomandibular Disorders: A Comprehensive Review

“…Acute episodes of gout disease are one of the most influential reasons for unfavourable health-related quality of life [ 139 , 140 , 141 ]. In a rat model, ozone therapy indicated a decrease in the degree of edematous ankle swelling, pro-inflammatory cytokines, lipid peroxidation, the nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3), procaspase-1, caspase-1, interleukin-1β synovial tissue levels with an enhancement of antioxidant defence system [ 142 ]. In other murine models, Bilge et al demonstrated that ozone therapy raises the levels of antioxidant enzymes, including oxidative shock proteins (hemo-oxygenase-1), Interleukin 4 and Interleukin 10, TGF-β, NO endorphin, adrenocorticotropic hormone (ACTH), and cortisol levels [ 141 ].…”

Ozone therapy for skin diseases: Cellular and molecular mechanisms

“…Ozone damages four polypeptide chains on viral capsid proteins, damages RNA, disrupts the reproductive cycle, and inhibits viral replication 76 . However, ozone has been shown to oxidise intracellular cysteine residues 77 . The NOD‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome is a cytoplasmic complex responsible for the production of IL‐1β and IL‐18 and plays a vital role in initiating inflammatory processes, including viral infections.…”

“… 76 However, ozone has been shown to oxidise intracellular cysteine residues. 77 The NOD‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome is a cytoplasmic complex responsible for the production of IL‐1β and IL‐18 and plays a vital role in initiating inflammatory processes, including viral infections. Reportedly, ozone exhibits anti‐inflammatory activity by modulating the NLRP3 inflammasome.…”