P-Cadherin and WNT5A expression in assessment of lymph node metastasis in oral squamous cell carcinoma

Khan, Wafa; Haragannavar, Vanishri C; Rao, Roopa S; Prasad, Kavitha; Sowmya, S; Augustine, Dominic; Patil, Shankargouda

doi:10.1007/s00784-021-03996-4

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…The subsequent screening step by both title and abstract resulted in 23 studies being included. Full‐text screening of the included studies resulted in 14 being selected for review 1,4,6,17,37,44,49–58 . A flowchart that depicts the screening process is displayed in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Genes involved in metastasis in oral squamous cell carcinoma: A systematic review

Mohtasham,

Zarepoor,

Shooshtari

et al. 2024

Health Science Reports

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Background and AimsOral squamous cell carcinoma is the most prevalent malignancy in the oral cavity, with a significant mortality rate. In oral squamous cell carcinoma patients, the survival rate could decrease because of delayed diagnosis. Thus, prevention, early diagnosis, and appropriate treatment can effectively increase the survival rate in patients. In this systematic review, we discussed the role of different genes in oral squamous cell carcinoma metastasis. Herein, we aimed to summarize clinical results, regarding the potential genes that promote oral squamous cell carcinoma metastasis.MethodsThis systematic review was carried out under the Preferred Reporting Items for Systematic Reviews and Meta‐Analysis guidelines. An electronic search for all relevant articles published in English between January 2018 and April 2022 was performed using Scopus, PubMed, and Google Scholar search engines. All original studies published in English were included, and we excluded studies that were in a non‐English language.ResultsA total of 4682 articles were found, of which 14 were relevant and detected significant genes in oral squamous cell carcinoma progression. These findings investigated the overexpression of interferon‐induced proteins with tetratricopeptide repeats 1 and 3 (IFIT1, IFT3), high‐mobility group A2 (HMGA2), transformed growth factor‐beta‐induced, lectin galactoside‐binding soluble 3 binding protein (LGALS3BP), bromodomain containing 4, COP9 signaling complex 6, heterogeneous nuclear ribonucleoproteins A2B1 (HNRNPA2B1), 5′−3′ exoribonuclease 2 (XRN2), cystatin‐A (CSTA), fibroblast growth factors 8 (FGF8), forkhead box P3, cadherin‐3, also known as P‐cadherin and Wnt family member 5A, ubiquitin‐specific‐processing protease 7, and retinoic acid receptor responder protein 2 genes lead to promote metastasis in oral squamous cell carcinoma. Overexpression of some genes (IFIT1, 3, LGALS3BP, HMGA2, HNRNPA2B1, XRN2, CSTA, and FGF8) was proven to be correlated with poor survival rates in oral squamous cell carcinoma patients.ConclusionStudies suggest that metastatic genes indicate a poor prognosis for oral squamous cell carcinoma patients. Detecting these metastatic genes in oral squamous cell carcinoma patients may be of predictive value and can also facilitate assessing oral squamous cell carcinoma development and its response to treatment.

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Section: Resultsmentioning

confidence: 99%

Genes involved in metastasis in oral squamous cell carcinoma: A systematic review

Mohtasham,

Zarepoor,

Shooshtari

et al. 2024

Health Science Reports

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“…Moreover, the result of protein mass spectrometry showed that P-cadherin, a classical molecule mainly involved in cell–cell adhesion, was significantly enriched in PD-L1-positive sEVs (Figure S13). In some certain types of malignancies, such as breast cancer and oral squamous cell carcinoma (OSCC), high expression levels of P-cadherin were significantly correlated with lymph node metastasis . It may be suspected that P-cadherin was also involved in the adhesion of PD-L1-positive sEVs to lymphatic endothelial cells.…”

Section: Resultsmentioning

confidence: 99%

“…In some certain types of malignancies, such as breast cancer and oral squamous cell carcinoma (OSCC), high expression levels of Pcadherin were significantly correlated with lymph node metastasis. 36 It may be suspected that P-cadherin was also involved in the adhesion of PD-L1-positive sEVs to lymphatic endothelial cells. Recently, it has been reported that ICAM-1 and PD-L1 were co-expressed on the sEVs, and antibody blocking or RNAi knockdown of ICAM-1 reduced the interaction between sEVs and CD8 T cells.…”

Section: Different Adhesion Patterns Of Pd-l1-positive and Pd-l1-nega...mentioning

confidence: 99%

Aptamer-Assisted Traceless Isolation of PD-L1-Positive Small Extracellular Vesicles for Dissecting Their Subpopulation Signature and Function

Liu

et al. 2022

Anal. Chem.

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Small extracellular vesicles (sEVs) are heterogeneous membrane-bound vesicles that carry numerous bioactive molecules. Studies have reported that sEVs carrying PD-L1 on the surface could contribute to immunosuppression; however, the precise mechanisms are unclear. To fully dissect their mode of action, it requires qualified methods to specifically isolate natural PD-L1-positive sEVs from heterogeneous sEVs. This study reported an aptamer-assisted capture-and-release strategy for traceless isolation of PD-L1-positive sEVs. The PD-L1 aptamer-anchored magnetic microspheres enable the specific capture of PD-L1-positive sEVs. The traceless release of captured PD-L1-positive sEVs was triggered by competition of complementary oligonucleotides, endowing the obtained label-free PD-L1-positive sEVs with natural properties. Benefited from this traceless isolation strategy, the distinct molecule profiles in adhesion and immuno-regulation between PD-L1-positive and PD-L1-negative sEVs were revealed. Compared to PD-L1-negative sEVs, PD-L1-positive sEVs were much more concentrated in cadherin binding, accompanied by increased adhesion to lymphatic endothelial cells and T cells but decreased adhesion to the extracellular matrix. Moreover, PD-L1-positive sEVs could transfer their enriched immunosuppressive “synapse”-related proteins to antigen-presenting cells, thereby inducing a tolerogenic-like phenotype. In summary, the present work dissects the subpopulation signature and action mode of PD-L1-positive sEVs for the first time and provides a general approach to the traceless isolation of sEV subpopulations.

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“…HNSCC has become a global health concern due to its high invasiveness and prone to lymph node metastasis [32]. The treatment needs to be improved for patients with advanced-stage, recurrent or metastatic HNSCC.…”

Section: Discussionmentioning

confidence: 99%

Elucidating the Characteristics and Functional Significance of Disulfidptosis-related Genes in Head and Neck Squamous Cell Carcinoma

Zhang

Zhao²,

Zhu³

et al. 2023

Preprint

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Disulfidptosis is a newfound programmed cell death (PCD) mode characterized by disulfide stress. Several computer-aided bioinformatic analyses were performed to elucidate the characteristics and functional significance of disulfidptosis-related genes in head and neck squamous cell carcinoma (HNSCC). The relative compositions of cells in the tumor microenvironment (TME), mutant landscape, lasso regression analysis, and predicted clinical outcome were performed by analyzing bulk RNA-sequence data. The prognostic model was verified by qRT-PCR. Besides, single-cell sequence data (scRNA) was analyzed by Seurat, CopyKAT, and monocle2 to reveal the expression characteristics of disulfidptosis-related genes. Moreover, the spatial distribution characteristics of each cell subgroup in the section and the functional significance of cancer-associated fibroblasts (CAFs) were clarified by STUtility, SpaCET, and SPATA2. Here, two clusters with different expression characteristics of disulfidptosis-related genes were identified. Cluster 1 (C1) patients had a worse prognosis and a higher proportion of stromal cells but lower effector T cell infiltration than cluster 2 (C2). A novel prognostic model was established and verified in our patient cohort. Additionally, diploid and inflammatory CAFs showed higher disulfidptosis-related gene expression levels. Furthermore, disulfidptosis-related genes exhibited extensive and differential spatial expression on tissue sections. Collectively, our study may contribute to revealing the function of disulfidptosis, and improve the expansion of knowledge of crosstalk between cancer cells and CAFs.

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P-Cadherin and WNT5A expression in assessment of lymph node metastasis in oral squamous cell carcinoma

Cited by 10 publications

References 33 publications

Genes involved in metastasis in oral squamous cell carcinoma: A systematic review

Genes involved in metastasis in oral squamous cell carcinoma: A systematic review

Aptamer-Assisted Traceless Isolation of PD-L1-Positive Small Extracellular Vesicles for Dissecting Their Subpopulation Signature and Function

Elucidating the Characteristics and Functional Significance of Disulfidptosis-related Genes in Head and Neck Squamous Cell Carcinoma

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