p-Coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS

Silina, Yuliya E.; Fink‐Straube, Claudia; Hanselmann, R.; Peuschel, Henrike; Volmer, Dietrich A.

doi:10.1016/j.jchromb.2016.03.005

Cited by 11 publications

(4 citation statements)

References 42 publications

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“…Specifically, CA9 and GLUT-1 often lead to false negatives, arising from fluctuation errors, in patient specimens. Therefore, from a biological plausibility standpoint, CA9 and GLUT-1 cannot be considered accurate hypoxia biomarkers [14, 15]. In contrast, pimonidazole is more specific for hypoxia [16]; however, because it needs to be pre-administered, it requires a moderate invasive procedure and cannot be used in retrospective studies.…”

Section: Introductionmentioning

confidence: 99%

ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines

et al. 2019

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Background Hypoxia is an important factor that contributes to tumour aggressiveness and correlates with poor prognosis and resistance to conventional therapy. Therefore, identifying hypoxic environments within tumours is extremely useful for understanding cancer biology and developing novel therapeutic strategies. Several studies have suggested that carbonic anhydrase 9 (CA9) is a reliable biomarker of hypoxia and a potential therapeutic target, while pimonidazole has been identified as an exogenous hypoxia marker. However, other studies have suggested that CA9 expression is not directly induced by hypoxia and it is not expressed in all types of tumours. Thus, in this study, we focused on endoplasmic reticulum disulphide oxidase 1α (ERO1α), a protein that localises in the endoplasmic reticulum and is involved in the formation of disulphide bonds in proteins, to determine whether it could serve as a potential tumour-hypoxia biomarker. Methods Using quantitative real-time polymerase chain reaction, we analysed the mRNA expression of ERO1α and CA9 in different normal and cancer cell lines. We also determined the protein expression levels of ERO1α and CA9 in these cell lines by western blotting. We then investigated the hypoxia-inducible ERO1α and CA9 expression and localisation in HCT116 and HeLa cells, which express low (CA9-low) and high (CA9-high) levels of CA9, respectively . A comparative analysis was performed using pimonidazole, an exogenous hypoxic marker, as a positive control. The expression and localisation of ERO1α and CA9 in tumour spheres during hypoxia were analysed by a tumour sphere formation assay. Finally, we used a mouse model to investigate the localisation of ERO1α and CA9 in tumour xenografts using several cell lines. Results We found that ERO1α expression increased under chronic hypoxia. Our results show that ERO1α was hypoxia-induced in all the tested cancer cell lines. Furthermore, in the comparative analysis using CA9 and pimonidazole, ERO1α had a similar localisation to pimonidazole in both CA9-low and CA9-high cell lines. Conclusion ERO1α can serve as a novel endogenous chronic hypoxia marker that is more reliable than CA9 and can be used as a diagnostic biomarker and therapeutic target for cancer. Electronic supplementary material The online version of this article (10.1186/s12885-019-5727-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines

et al. 2019

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“…The elemental compositions of the fragment ions were C 10 H 8 O 4 , C 9 H 5 O 4 , C 9 H 6 O 3 ,C 8 H 5 O 3 , and C 7 H 4 O 3 , respectively. The peak of compound 50 was identified as coumaric acid‐4‐ O ‐ β ‐ D ‐glucoside (C 15 H 18 O 8 ) 34–37 . Product ion scan of the deprotonated molecule [M–H] − at m/z 325.0923 afforded eight major fragment ions at m/z 179.05584 ([M–H–C 9 H 6 O 2 ] − , 161.04541 ([M–H–C 9 H 8 O 3 ] − ), 145.02980 ([M–H–C 6 H 12 O 6 ] − ), 143.03489 ([M–H–C 9 H 8 O 3 –H 2 O] − ), 119.03458 ([M–H–C 11 H 10 O 4 ] − ), 117.03458 ([M–H–C 7 H 12 O 7 ] − ), 113.02411 ([M–H–C 10 H 12 O 5 ] − ), and 101.02403 ([M–H–C 11 H 10 O 4 –H 2 O] − ).…”

Section: Resultsmentioning

confidence: 99%

Identification of chemical constituents in pomegranate seeds based on ultra‐high‐performance supercritical fluid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Chen

Yang

et al. 2023

Rapid Comm Mass Spectrometry

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Pomegranate seeds are a potential source of bioactive compounds. Nonetheless, most pomegranate seeds are discarded in the food processing industry, likely due to the lack of convincing data on their component analysis.MethodsTo reveal the main chemical constituents of pomegranate seeds, a reliable and sensitive method based on ultra‐high‐performance supercritical fluid chromatography coupled with electrospray ionization and quadrupole time‐of‐flight mass spectrometry (MS) was developed. A time‐dependent MSE data acquisition mode was applied to acquire the mass spectrometric data. The chemical constituents were identified by an automatic retrieval of a traditional Chinese medicine library and relevant literature.ResultsA total number of 59 compounds, including fatty acids, sterols, vitamins, cerebrosides, phospholipids, flavonoids, phenylpropanoids, and others, were tentatively identified. Their possible fragmentation pathways and characteristic ions were proposed and elucidated.ConclusionsThe findings of this study, along with the developed methodology, could provide a reference for basic research on the pharmacodynamic substances of pomegranate seeds and shed light on their potential nutritional and therapeutic applications in the future.

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“…Peak 4 had the mass spectrum at m / z 153.0186 (C 7 H 6 O 4 ) [M − H] − , the product ion was 109.0287 [M − H − CO 2 ] − , which could be identified as 2, 5-Dihydroxybenzoic acid. Peak 8 had the mass spectrum at m / z 163.0397 (C 9 H 8 O 3 ) [M − H] − , the product ions were 119.0495 [M − H − CO 2 ] − , therefore, it could be identified as p-coumaric acid [26]. Peak 9 had the parent ion at m / z 193.0507 (C 10 H 10 O 4 ) [M − H] − , the target product ions 135.0411 and 89.0324 can be obtained in the MS/MS spectra, which could be used to identify peak 9 as ferulic acid [6].…”

Section: Resultsmentioning

confidence: 99%

A Single Standard to Determine Multi-Components Method Coupled with Chemometric Methods for the Quantification, Evaluation and Classification of Notopterygii Rhizoma et Radix from Different Regions

Azietaku

et al. 2019

Molecules

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An ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry is used to identify 33 compounds in Notopterygii rhizoma and radix, after which a single standard to determine multi-components method is established for the simultaneous determination of 19 compounds in Notopterygii rhizoma and radix using chlorogenic acid and notopterol as the internal standard. To screen the potential chemical markers among Notopterygii rhizoma and radix planted in its natural germination area and in others, the quantitative data of 19 compounds are analyzed via partial least-squares discriminant analysis (PLS–DA). Depending on the variable importance parameters (VIP) value of PLS–DA, six compounds are selected to be the potential chemical markers for the discrimination of Notopterygii rhizoma and radix planted in the different regions. Furthermore, the Fisher’s discriminant analysis is used to build the models that are used to classify Notopterygii rhizoma and radix from the different regions based on the six chemical markers. Experimental results indicate that Notopterygii rhizoma and radix planted in the Sichuan province are distinguished successfully from those in other regions, reaching a 96.0% accuracy rating. Therefore, a single standard to determine multi-components method combined with a chemometrics method, which contains the advantages such as simple, rapid, economical and accurate identification, offers a new perspective for the quantification, evaluation and classification of Notopterygii rhizoma and radix from the different regions.

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p-Coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS

Cited by 11 publications

References 42 publications

ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines

ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines

Identification of chemical constituents in pomegranate seeds based on ultra‐high‐performance supercritical fluid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

A Single Standard to Determine Multi-Components Method Coupled with Chemometric Methods for the Quantification, Evaluation and Classification of Notopterygii Rhizoma et Radix from Different Regions

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