2013
DOI: 10.1111/jvp.12040
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P‐glycoprotein in sheep liver and small intestine: gene expression and transport efflux activity

Abstract: The role of the transporter P-glycoprotein (P-gp) in the disposition kinetics of different drugs therapeutically used in veterinary medicine has been demonstrated. Considering the anatomo-physiological features of the ruminant species, the constitutive expression of P-gp (ABCB1) along the sheep gastrointestinal tract was studied. Additionally, the effect of repeated dexamethasone (DEX) administrations on the ABCB1 gene expression in the liver and small intestine was also assessed. The ABCB1 mRNA expression was… Show more

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Cited by 15 publications
(12 citation statements)
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“…Alternatively, the transport of antibiotics into the intestine of cattle may be caused by intestinal efflux transporters. The existence of these transporters has been shown for other ruminants (Ballent et al ., ). These intestinal mucosal efflux proteins are capable of transporting antibiotics directly from the circulation to the intestinal lumen (Shugarts & Benet, ).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Alternatively, the transport of antibiotics into the intestine of cattle may be caused by intestinal efflux transporters. The existence of these transporters has been shown for other ruminants (Ballent et al ., ). These intestinal mucosal efflux proteins are capable of transporting antibiotics directly from the circulation to the intestinal lumen (Shugarts & Benet, ).…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, it is unknown, but hypothesized that different antibiotic properties including lipophilicity, protein binding, interactions with intestinal transporters, and ionization may affect their ability to penetrate the intestinal epithelium and remain active in the GIT after intramuscular or subcutaneous injection. Some antimicrobials may enter the intestine via passive diffusion, while others may be transported via mucosal efflux pumps, which are known to exist in ruminants (Ballent et al, 2013). These mechanisms have not been studied in cattle.…”
mentioning
confidence: 99%
“…In both treated groups, the inhibition seemed to last longer than in the respective control groups ( Figure 2). e use of rhodamine-123 as a P-gp-substrate and verapamil as a P-gp-inhibitor is well established [42][43][44]. However, a significant efflux of rhodamine-123 mediated by other transport proteins, such as multidrug-related-proteins (MRPs), has to be considered [45,46].…”
Section: Discussionmentioning
confidence: 99%
“…Gene expression analysis using real-time RT-PCR has been employed for studying the differences in inter-species relationships in veterinary medicine, hence the need for conducting research in specific animal species to obtain accurate information of the constitutive expression of multi-drug transporters [21]. Another example is the gene expression and transport efflux activity of P-glycoprotein in sheep liver and small intestines (Table 2) [21].…”
Section: Discussionmentioning
confidence: 99%
“…Another example is the gene expression and transport efflux activity of P-glycoprotein in sheep liver and small intestines (Table 2) [21].…”
Section: Discussionmentioning
confidence: 99%