P-gp expression in brown trout erythrocytes: evidence of a detoxification mechanism in fish erythrocytes

Abstract

Blood is a site of physiological transport for a great variety of molecules, including xenobiotics. Blood cells in aquatic vertebrates, such as fish, are directly exposed to aquatic pollution. P-gp are ubiquitous “membrane detoxification proteins” implicated in the cellular efflux of various xenobiotics, such as polycyclic aromatic hydrocarbons (PAHs), which may be pollutants. The existence of this P-gp detoxification system inducible by benzo [a] pyrene (BaP), a highly cytotoxic PAH, was investigated in the nucleated erythrocytes of brown trout. Western blot analysis showed the expression of a 140-kDa P-gp in trout erythrocytes. Primary cultures of erythrocytes exposed to increasing concentrations of BaP showed no evidence of cell toxicity. Yet, in the same BaP-treated erythrocytes, P-gp expression increased significantly in a dose-dependent manner. Brown trout P-gp erythrocytes act as membrane defence mechanism against the pollutant, a property that can be exploited for future biomarker development to monitor water quality.

BCRP and P-gp relay overexpression in triple negative basal-like breast cancer cell line: a prospective role in resistance to Olaparib

“…Protein concentration was determined using a protein assay kit (Coomassie Plus – The Better Bradford Assay TM Kit, Thermo Scientific, France) according to the manufacturer’s protocol. Western blotting was then carried out according to Valton et al , 2013 18 . Briefly, each lysate was diluted in a sample buffer (1.25 M tris-HCl, 20% sodium dodecyl sulfate, 20% glycerol, 2 M dithiothreitol, 0.5% bromophenol blue, pH 6.8) to a protein concentration of 3 μg/μL, and 20 μL of each diluted sample were electrophoresed.…”

“…MDR transporters belong to the “ATP-binding cassette” (ABC) superfamily of proteins, which were highly conserved during evolution. Indeed, these proteins are naturally expressed in all living organisms from prokaryotes to eukaryotes 18 . The two major MDR proteins, Permeability-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), which belong to the ABCB and ABCG subfamily, respectively, have been showed to be frequently expressed in human cancer.…”

Application of Salmonids (Salmonidae) in the Biomonitoring of Aquatic Environment

“…Зростання їхнього рівня спостерігається за впливу багатьох як органічних полютантів, зокрема поліциклічних ароматичних вуглеводнів -креозоту, бенз(а)пірену тощо, пестицидів, так і неорганічних сполук. Згідно з дослідженнями, значна їх експресія відмічається у тканинах з індукованою неоплазією, що вказує на перспективність Р-глікопротеїнів як біохімічних маркерів онкогенних процесів або потенційної сприйнятливості до них організму риб [12,[50][51][52][53][54][55].…”

Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution

“…Indeed, pollutants may be found directly in the blood or stored in organs, tissues and cells from which they may be gradually released into the circulation. Changes in the intrinsic parameters of blood cells [ 5 ] or in blood biochemistry [ 6 ] can be exploited as biomarkers of environmental pollution.…”

“…Therefore, MDR transporters are currently considered as a major element in the mechanism of chemoimmunity [ 7 ]. Moreover, MDR proteins are ubiquitous, belonging to the evolutionarily conserved family of the ATP-binding cassette (ABC) proteins found in most living organisms, from bacteria [ 8 ], parasites [ 9 , 10 ], free-living protozoa [ 11 , 12 ], aquatic invertebrates and fish [ 5 , 13 , 14 , 15 ], Drosophila [ 16 , 17 ], plants [ 18 , 19 ] to mammals [ 7 ]. In man, the three principal types of MDR proteins identified are: (i) Permeability glycoprotein (P-gp) encoded by ABCB1 or MDR1 gene; (ii) MDR-associated protein (MRP) encoded by ABCC genes ( ABCC1 , ABCC2 , plus probably ABCC3-6 and ABCC10-11 ); (iii) Breast Cancer Resistance Protein (BCRP) encoded by ABCG2 gene.…”