P-gp expression levels in the erythrocytes of brown trout: a new tool for aquatic sentinel biomarker development

Abstract

This biological tool may offer considerable advantages since it provides an effective response to the increasing need for an early biomarker.

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

“…The meaning of such an activity is poorly documented and still not understood. The interest in the metabolic activity of red blood cells has recently increased, aimed at use blood cells as new tool for aquatic sentinel biomarker development (Maceda-Veiga et al 2015 ;Valton et al 2017).…”

“…Gundersen et al 2008;Jacobs et al 2014;Farkas et al 2017). The analysis of target gene transcription can be performed on red blood cells, since they are nucleated at fish (Valton 2017).…”

Chemical biology fluorescent tools for in vitro investigation of the multidrug resistant P-glycoprotein (P-gp) expression in tumor cells

“…Although the design of such constructs can be quite challengingthe ligand affinity for the target must be minimally altered aer conjugation to the uorophore -specically designing P-gp targeted uorescent conjugates would be highly benecial to the eld and complement the various uorescent substrates reported to date for studying P-gp in in vitro functional assays. [15][16][17][18] In this report, building on our expertise in studying P-gp in preclinical in vitro conditions, especially on cancer models, [19][20][21][22] we describe the design and detail the synthesis of a series of Pgp targeted conjugates, for which a small peptidic core displaying uorophores with varying optical properties was derivatized with aliphatic and aromatic groups. Based on ow cytometry and imaging-based experiments, we also contrast their in vitro performance and demonstrate their potential for P-gp detection and quantication in a cell model.…”

“…In this report, building on our expertise in studying P-gp in preclinical in vitro conditions, especially on cancer models, 19–22 we describe the design and detail the synthesis of a series of P-gp targeted conjugates, for which a small peptidic core displaying fluorophores with varying optical properties was derivatized with aliphatic and aromatic groups. Based on flow cytometry and imaging-based experiments, we also contrast their in vitro performance and demonstrate their potential for P-gp detection and quantification in a cell model.…”

LightSpot®-FL-1 Fluorescent Probe: An Innovative Tool for Cancer Drug Resistance Analysis by Direct Detection and Quantification of the P-glycoprotein (P-gp) on Monolayer Culture and Spheroid Triple Negative Breast Cancer Models

“…MDR proteins, and the P-gp in particular, were highly conserved during the evolutionary process and are ubiquitous. In fact, they are naturally expressed in a wide variety of living organisms from prokaryotes to eukaryotes, such as bacteria, protozoa, fish, Drosophila and mammals [2][3][4][5][6]. In humans, MDR proteins are commonly studied because of their involvement in tumoral multidrug resistance mechanisms [7,8].…”