p(HEMA)-RR241 hydrogel membranes with micron network for IgG depletion in proteomic studies

Kuşat, Kevser; Bağlamış, Selami; Kuru, Cansu İlke; Ulucan, Fulden; Uygun, Murat; Akgöl, Sinan

doi:10.1080/09205063.2022.2045666

Cited by 2 publications

(1 citation statement)

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“…Nanoparticles with unique physical, chemical, optical and electrical properties are frequently used in biotechnological applications. In addition, their ability to recognize biomolecules specifically has been the reason for their preference in biotechnological [ 6 – 9 ]. In addition, nanoparticles provide advantages such as minimum diffusion limitation, maximum surface area per unit mass, and high enzyme loading capacity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Study of silanized nanostructures with immobilized fumarase for production of L-malate

ATAY¹,

KUŞAT²,

AKGÖL³

2022

Turkish Journal of Chemistry

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The conversion of fumaric acid into L-malate by fumarase immobilized on silanized nanostructures was analyzed experimentally. The enzyme was bound to the silanized nanostructures. We carried out scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) analysis, zeta size analysis and surface area calculation for the characterization of the nanostructures. The effect of initial enzyme concentration and pH on immobilization procedure were investigated and the change of Michaelis-Menten constants (K m and V max ) with immobilization was examined. The change in the storage stability of the enzyme by immobilization was also investigated. The stability of the immobilized enzyme was very good. We observed that the fumarase was bound to silanized nanostructures [p(HEMA)-3-MTES] in much greater amounts. We have compared the activities of free fumarase and immobilized fumarase and we have observed a significant increase in the activity of the fumarase after immobilization for L-malate production. Moreover, we came to the conclusion that this activity can be better preserved for 30 days compared to free fumarase.

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Section: Introductionmentioning

confidence: 99%