P024 High expression of TH2-like cytokines in dermatitis herpetiformis

Caproni, Marzia; Salvatore, E; Fabbri, Paolo

doi:10.1016/s0926-9959(97)89498-6

Cited by 2 publications

(5 citation statements)

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“…Most of these cells appeared degranulated on staining for their specific proteins (MPO and EG1/ EG2, respectively). As in other chronic bullous diseases such as DH or BP, [16][17][18] our results show the presence of a T-cell infiltrate in LAD lesions. These T cells were not as well represented as the granulocytes but they were activated, as they expressed HLA-DR class II molecules, adhesion molecules (ICAM-1, ICAM-3 and VCAM-1), IL-2R (CD25 þ) and a more considerable staining for CD30.…”

Section: Discussionsupporting

confidence: 82%

“…These data are in agreement with what has been observed in DH, in which an IgA Th2-related mechanism has also been postulated. 18 This comparison is intriguing because the pathogenesis of DH is clearly different from the pathogenesis of LAD, DH being associated with a gluten-sensitive enteropathy and the origin of the DH IgA (maybe immune complexes) not yet being well defined.…”

Section: Discussionmentioning

confidence: 99%

“…An intense staining for CD1a was detected in the epidermis and a few CD1a þ cells were also found in the dermis. MPO þ cells were demonstrated in perivascular areas of the superficial dermis (mean 17·6, range [16][17][18][19][20]. A focal staining for EG1 and EG2 (mean 2, range 0-4) was detected in the superficial dermis, while a larger number of tryptase-positive cells (mean 22·3, range [19][20][21][22][23][24][25][26] was found in the papillary and perivascular superficial dermis.…”

Section: Cells and Cytokines In Lad 1075mentioning

confidence: 95%

“…17 Such an infiltrate, through a T-cell-mediated immune reaction, has been found to participate in the recruitment of neutrophil and eosinophil granulocytes. 18 As histological studies of lesional skin of patients with LAD have shown the presence of infiltrating cells, represented by lymphocytes, eosinophils and neutrophils, it is likely that these cells play a part in the pathogenetic mechanism of the cutaneous lesions, and they may be responsible for the expression of a particular clinical or immunological subtype of LAD.…”

mentioning

confidence: 99%

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The role of lymphocytes, granulocytes, mast cells and their related cytokines in lesional skin of linear IgA bullous dermatosis

Caproni

Rolfo

Bernacchi

et al. 1999

British Journal of Dermatology

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Linear IgA bullous dermatosis (LAD) is an acquired, heterogeneous, subepidermal blistering disease characterized by linear IgA deposits at the dermoepidermal basement membrane zone (BMZ), often with circulating IgA antibodies to the BMZ. The pathogenetic mechanism, possibly related to the immunophenotype of infiltrating cells, as well as the potential role of cytokines in determining bullous lesions, have not yet been elucidated. An immunohistochemical study was performed with a large panel of monoclonal antibodies [to CD3, CD4, CD8, CD25, CD1a, CD30, CD54, CD50, endothelial leucocyte adhesion molecule-1, vascular cell adhesion molecule-1, myeloperoxidase (MPO), eosinophil cationic protein EG1 and EG2, tryptase, HLA-DR, human interleukin (IL)-3, human IL-5, human IL-8, human IL-4, tumour necrosis factor (TNF)-alpha, interferon (IFN)-gamma and granulocyte/macrophage colony-stimulating factor] using the alkaline phosphatase-antialkaline phosphatase procedure on lesional and perilesional skin of nine patients (one male, eight female; age range 8 months-80 years) with clinical, histological and immunofluorescent proven LAD. The predominant infiltrating cells, distributed mostly inside and below the bullae, were neutrophils and eosinophils which showed intense activation (MPO +, EG1 +, EG2 +). The lymphocytic infiltrate, consisting principally of CD4 +, HLA-DR + and CD30 + T cells, had a predominantly perivascular distribution. Proinflammatory cytokines, such as TNF-alpha and IFN-gamma, showed a moderate focal expression on the dermal perivascular sites; IL-8 was found to have a particularly intense staining on all the epidermal cell layers and at perivascular and vascular sites. Other cytokines, such as IL-4 and IL-5, showed a prevalent intracytoplasmic staining on some cells of the dermal infiltrate (probably mastocytes and lymphocytes), and at the dermal-epidermal separation sites there was also an intense scattered distribution of IL-5. The specific tissue lesions of LAD may be the consequence of the IgA deposits at the BMZ and also of the release of these cytokines together with tissue damage enzymes derived from neutrophils or eosinophils.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Cells and Cytokines In Lad 1075mentioning

confidence: 95%

mentioning

confidence: 99%

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The role of lymphocytes, granulocytes, mast cells and their related cytokines in lesional skin of linear IgA bullous dermatosis

Caproni

Rolfo

Bernacchi

et al. 1999

British Journal of Dermatology

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“…Recently, several studies have suggested cytokine involvement in autoimmune bullous disorders. Cytokines have been shown to play a part in bullous pemphigoid (Lee et al, 1993;Ameglio et al, 1998) and dermatitis herpetiformis (Caproni et al, 1998). Systemic treatment with neutralizing antibodies to murine IL-1 or IL-6 completely blocked the development of blisters in BALB/C mice injected with IgG directed against the 180 kDa bullous pemphigoid antigen.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Expression of Interleukin-1α and Tumor Necrosis Factor-α mRNA in Pemphigus Vulgaris: Interleukin-1α and Tumor Necrosis Factor-α are Involved in Acantholysis

Feliciani

Toto

Pour

et al. 2000

Journal of Investigative Dermatology

167

145

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Keratinocyte-derived cytokines have been implicated in the pathogenesis of a number of skin diseases. In this study we examined the possible role of keratinocyte-derived cytokines in the development of acantholysis in pemphigus vulgaris. Nineteen patients with pemphigus vulgaris, demonstrating the characteristic clinical, pathologic, and immunopathologic findings were studied. In situ immunolabeling demonstrated the presence of two cytokines interleukin-1alpha and tumor necrosis factor-alpha, in lesional and perilesional areas. Results were confirmed by reverse transcriptase-polymerase chain reaction, demonstrating overexpression of both cytokines in vivo. To study the role of these cytokines in the pathogenesis of pemphigus vulgaris both in vitro and in vivo studies were performed. The results of the in vitro study demonstrated that pemphigus vulgaris IgG induced interleukin-1alpha and tumor necrosis factor-alpha mRNA in the skin. The potential pathogenic role of these mediators was demonstrated by a blocking study using antibodies against human interleukin-1alpha and tumor necrosis factor-alpha in keratinocytes cultures. A combination of anti-interleukin-1alpha and anti-tumor necrosis factor-alpha antibodies inhibited in vitro pemphigus vulgaris IgG induced acantholysis. To confirm the role of interleukin-1 and tumor necrosis factor-alpha in pemphigus, we utilized passive transfer studies using interleukin-1 deficient mice (ICE-/-, interleukin-1beta-/-) and tumor necrosis factor-alpha receptor deficient mice (TNFR1R2-/-). Both groups demonstrated a decreased susceptibility to the passive transfer of pemphigus. Our data support the role of cytokines interleukin-1 and tumor necrosis factor-alpha in the pathogenesis of pemphigus vulgaris.

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P024 High expression of TH2-like cytokines in dermatitis herpetiformis

Cited by 2 publications

References 0 publications

The role of lymphocytes, granulocytes, mast cells and their related cytokines in lesional skin of linear IgA bullous dermatosis

The role of lymphocytes, granulocytes, mast cells and their related cytokines in lesional skin of linear IgA bullous dermatosis

In Vitro and In Vivo Expression of Interleukin-1α and Tumor Necrosis Factor-α mRNA in Pemphigus Vulgaris: Interleukin-1α and Tumor Necrosis Factor-α are Involved in Acantholysis

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