P1-antigen-containing avian egg whites as inhibitors of P adhesins among wild-type Escherichia coli strains from patients with urosepsis

Abstract

Among 58 Escherichia coli urosepsis isolates, Pl-antigen-containing dove and pigeon egg whites were significantly more effective inhibitors of P-adhesin-specific agglutination than were chicken egg whites or globoside. Globoside's inefficacy may have resulted from a proadherence effect of globoside's lipid tail. Adhesin phenotypes determined with dove and pigeon egg whites as agglutination inhibitors corresponded closely with phenotypes defined by comparative hemagglutination of human P1 and p erythrocytes. These data suggest that avian Pl-antigen-containing substances may provide a useful alternative method for P adhesin inhibition among uropathogenic E. coli strains.

Diversity of Hemagglutination Phenotypes among P-Fimbriated Wild-Type Strains of Escherichia coli in Relation to papG Allele Repertoire

“…Prior to experiment 2, to exclude strains with non-P adhesins, microscope slide HA assays (24) were used to determine each strain's mannose-resistant HA (MRHA) titer for human A 1 P 1 and p erythrocytes ( Table 2). If HA was observed with either cell type, inhibition of HA by pigeon egg white (PEW) and 3% bovine serum albumin (Sigma) in PBS was assessed at a bacterial concentration four times that required for HA (19,20). Strains that exhibited PEW-inhibitable MRHA of A 1 P 1 erythrocytes but no MRHA of p erythrocytes were considered to express P adhesins; those that exhibited PEW-inhibitable MRHA of both A 1 P 1 and p erythrocytes were considered to express NPMR (formerly X) adhesins (19,20).…”

Hemagglutination ability and adherence to the Buffalo green monkey kidney cell line of uropathogenic Escherichia coli

“…Egg whites of pigeons, doves, and members of the parrot family exhibit high levels of the digalactoside-containing PI antigen and they are potent inhibitors of P adhesins from E. coli 596 (Johnson et al 1992). Albeit P-fimbriae of strain J96 are not representative of the P adhesins of most uropathogenic strains (Striimberg et al 1990), Johnson & Ross (1993) have found that avian P1-antigen-containing glycoproteins strongly inhibit P adhesins of wild-type E. coli strains from patients with urosepsis. These data correlate with the results obtained in the present research which have shown that P,-antigen-containing pigeon egg white inhibits the adherence of wild-type P-fimbriated E. coli strains isolated from the urine of patients with uroinfection to BGM cells, as well as hemagglutination caused by these strains.…”

“…Isolates were considered to express P-fimbriae if HA was positive with human erythrocytes and inhibition of HA was positive with pigeon egg white, which was confirmed by agglutination of receptorcoated latex beads (Johnson & Ross 1993). Isolates were considered to express ONAP (0 negative, A positive) adhesin if they agglutinated sheep and human Alpl erythrocytes but did not agglutinate human OPI MM, OplNN, OP2MM, OP2NN erytrocytes and Gal-Gal-latex beads, and to express an X-adhesin if they agglutinated human erythrocytes but not Gal-Gal-latex beads (Lindstedt et al 1989).…”