P1013 Detection and characterisation of ESBLs in German Escherichia coli, isolated from animal, foods, and human origin between 2001–2006

Guerra, Beatriz; Avsaroglu, M. Dilek; Junker, Ernst; Schroeter, A.; Beutin, Lothar; Helmuth, Reiner

doi:10.1016/s0924-8579(07)70854-3

Cited by 9 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalences of ESBL-producing E. coli-positive fecal samples on mixed farms were 39.6% and 18.9% on beef cattle farms. This seems to be an increase, as such strains have only sporadically been described in Germany before (16). However, this could also be due to differences in the detection methods as in this study ESBLproducing E. coli strains were selected using enrichment and selective media (MCA containing cefotaxime).…”

Section: Discussionmentioning

confidence: 92%

“…However, this could also be due to differences in the detection methods as in this study ESBLproducing E. coli strains were selected using enrichment and selective media (MCA containing cefotaxime). Guerra et al (16), however, did not test samples from field trials, but received E. coli isolates from different labs wanting them to be typed. These isolates have not been selected with special media, which has an important effect on the results.…”

Section: Discussionmentioning

confidence: 99%

“…The data about ESBL-producing bacteria in food animals in Germany are very limited. To date, only one publication on the detection of ESBL-producing E. coli in food animals in Germany has been available (16). Therefore, this study was conducted to estimate the prevalence of ESBL-producing E. coli in healthy cattle, evaluating different ages and farm types.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Schmid¹,

Hörmansdorfer²,

Messelhäußer³

et al. 2013

Appl Environ Microbiol

137

135

View full text Add to dashboard Cite

c Extended-spectrum ␤-lactamase (ESBL)-producing Escherichia coli strains are believed to be widely distributed among humans and animals; however, to date, there are only few studies that support this assumption on a regional or countrywide scale. Therefore, a study was designed to assess the prevalence of ESBL-producing E. coli in dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs, and dust samples were analyzed for ESBL-producing E. coli using selective media. PCR was performed to screen for CTX-M and ampC resistance genes. A total of 598 samples yielded 196 (32.8%) that contained ESBL-producing E. coli, originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL-producing E. coli positive than samples from beef cattle farms (fecal samples, P < 0.001; boot swabs, P ‫؍‬ 0.014; and dust samples, P ‫؍‬ 0.041). A total of 183 isolates (93.4%) of 196 ESBL-producing E. coli-positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty-six additional isolates contained ampC genes, and 5 of the 46 isolates expressed a bla CMY-2 gene. The study shows that ESBL-producing E. coli strains are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence of bla CMY-2 in cattle in Germany.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Schmid¹,

Hörmansdorfer²,

Messelhäußer³

et al. 2013

Appl Environ Microbiol

137

135

View full text Add to dashboard Cite

show abstract

“…➀ Because antibiotic-resistant bacteria, including opportunistic pathogens mainly cause harmful effects by unexpectedly rapid proliferation under antibiotic treatment 5) rather than through the production of a specific toxin, a primer for producing gene of a specific toxin was not suitable to evaluate their risk; ➁ Because resistance to antibiotics can arise from mutations in the bacterial genome or through the acquisition of genes coding for resistance, 5) which only induce negligible variation in whole bacterial DNA, 1,29) taxonomical difference was not suitable to discriminate resistant bacteria from susceptible bacteria; ➂ Because there exist various mechanisms for acquired resistance, 30) a primer for a specific resistant gene could not cover whole resistant bacteria but could be used for certain resistant bacteria. 31) The method presented here was found to be effective and useful for the purpose of monitoring whole antibiotic-resistant bacteria in the environment and to evaluate their risk. A more convenient and secure method by which types of bacteria and their numbers can be identified and enumerated by the combined use of a most provable number method (MPN) and a MERFLP method without isolating bacteria have already been developed.…”

Section: Discussionmentioning

confidence: 93%

Application of multiple enzyme restriction fragment length polymorphism analysis and microchip electrophoresis for estimation of antibiotic-tolerant bacterial group

Watanabe

2008

J. Pestic. Sci.

View full text Add to dashboard Cite

A combined method of microchip electrophoresis and multiple enzyme restriction fragment length polymorphism analysis, by which bacterial phylogeny was estimated by comparing the measured multiple enzyme restriction fragment (MERF) to that of the theoretical MERF of various kinds of bacteria, was adapted to monitor an antibiotic-resistant bacterial group in field soils. One hundred and ninety bacteria showing polymyxin B tolerance were newly isolated from experimental field soils where a large amount of liquid livestock feces (600 t/ha/year, 120 t/haրyear) has been applied annually, and neighboring area where feces were not applied (0 t area). In the 0 t area, most bacteria grown on PP agar was Bacillus spp. (80%), which decreased to 21.7% in the 120 t field, and 15.6% in the 600 t field. As the percentages gram negative bacteria, Cytophagales, and Spirochaetales increased to 10.0% in the 120 t field, and 33.3% in the 600 t field, and 7 isolates from the 120 t field and 18 isolates from the 600 t field were ordinary bacteria inhabiting in animals and feces, they originated from livestock. As polymyxin B is bactericidal for gram-negative bacteria, some of the gram-negative bacteria isolated from both the feces-applied fields were supposed to be polymyxin B-resistant bacteria. © Pesticide Science Society of Japan Keywords: polymyxin B resistant bacteria, field soil, livestock feces, microchip electrophoresis, multiple enzyme restriction fragment length polymorphism analysis.

show abstract

“…We have detected ESBL-producing E. coli in livestock (5% of the animals tested). In Europe such strains have been only sporadically described in cattle: in Germany (Guerra et al, 2007 ), England (Horton et al, 2011 ), and Spain (Brinas et al, 2005 ). To our best knowledge this is the second report of ESBL-producing E. coli in cattle in France where Meunier et al ( 2006 ) reported three isolates (carrying either CTX-M-1 or CTX-M-15 ESBL) responsible for infections in cow.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of CTX-M Producing Escherichia coli in Soils, Cattle, and Farm Environment in France (Burgundy Region)

Hartmann¹,

Locatelli²,

Amoureux³

et al. 2012

Front. Microbio.

118

View full text Add to dashboard Cite

CTX-M [a major type of extended-spectrum beta-lactamase (ESBL)] producing Escherichia coli are increasingly involved in human infections worldwide. The aim of this study was to investigate potential reservoirs for such strains: soils, cattle, and farm environment. The prevalence of blaCTX-M genes was determined directly from soil DNA extracts obtained from 120 sites in Burgundy (France) using real-time PCR. blaCTX-M targets were found in 20% of the DNA extracts tested. Samples of cattle feces (n = 271) were collected from 182 farms in Burgundy. Thirteen ESBL-producing isolates were obtained from 12 farms and further characterized for the presence of bla genes. Of the 13 strains, five and eight strains carried blaTEM-71 genes and blaCTX-M-1 genes respectively. Ten strains of CTX-M-1 producing E. coli were isolated from cultivated and pasture soils as well as from composted manure within two of these farms. The genotypic analysis revealed that environmental and animal strains were clonally related. Our study confirms the occurrence of CTX-M producing E. coli in cattle and reports for the first time the occurrence of such strains in cultivated soils. The environmental competence of such strains has to be determined and might explain their long term survival since CTX-M isolates were recovered from a soil that was last amended with manure 1 year before sampling.

show abstract

P1013 Detection and characterisation of ESBLs in German Escherichia coli, isolated from animal, foods, and human origin between 2001–2006

Cited by 9 publications

References 0 publications

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Application of multiple enzyme restriction fragment length polymorphism analysis and microchip electrophoresis for estimation of antibiotic-tolerant bacterial group

Occurrence of CTX-M Producing Escherichia coli in Soils, Cattle, and Farm Environment in France (Burgundy Region)

Contact Info

Product

Resources

About