p16 gene methylation in colorectal cancer patients with long-term follow-up

Veganzones, Silvia; Rafael-Fernandez, Sara; Vidaurreta-Lázaro, Marta; de-la-Orden,; Mediero-Valeros, B.; Fernández, Cristina; Ml, Maestro-de las Casas

doi:10.4321/s1130-01082012000300002

Cited by 25 publications

(16 citation statements)

References 37 publications

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“…In our study, TP53 mutations were not tested. Veganzones-de-Castro et al working on colorectal cancer made the same observation as we did [48]. They found that patients with poorly differentiated tumors having CDKN2A promoter methylated presented longer free survival than those without CDKN2A methylation.…”

Section: Discussionsupporting

confidence: 85%

Prognostic factors in oral and oropharyngeal cancer based on ultrastructural analysis and DNA methylation of the tumor and surgical margin

et al. 2014

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Oral and oropharyngeal cancers are characterized by relatively low 5- year survival rates due to many factors, including local recurrence. The identification of new molecular markers may serve for the estimation of prognosis and thus augment treatment decisions and affect therapy outcome. The aim of this study was to describe the morphological characteristics and the DNA methylation status of the CDKN2A,CDH1, ATM, FHIT and RAR- genes in the central and peripheral part of the tumor and the surgical margin and evaluate their prognostic significance. 53 patients with oral and oropharyngeal cancer were enrolled to the prospective study, and had been primarily treated surgically. Correlations between morphological data, hypermethylation status and clinicopathological data, as well as prognosis, were assessed. Nuclei polymorphism highly correlated with T stage (p < 0.0001), N stage (p < 0.046), and metastases to the lymph nodes pN (p < 0.004 ). Also, the number of cells in irregular mitosis correlated with T stage (p < 0.004), and highly with pN (p < 0.009). The significance of CDKN2A hypermethylation as a good prognostic factor was also established in the Kaplan-Meir test. The ultrastructural analysis showed that none of the examined tumors had homogenous texture and that resection margin specimens clean in HE stained tissue samples frequently contained single tumor cells or few cells in groups surrounded by connective tissue. This indicates the superiority of electron microscopy over standard histopathological analysis. Thus, a combination of such morphological examination with epigenetic parameters described herein could result in the discovery of promising new prognostic markers of the disease.

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Section: Discussionsupporting

confidence: 85%

Prognostic factors in oral and oropharyngeal cancer based on ultrastructural analysis and DNA methylation of the tumor and surgical margin

et al. 2014

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“…Full texts and data integrity were then reviewed and another 249 articles were excluded. Finally, 27 clinical cohort studies were included in this meta-analysis (Liang et al, 1999;Wiencke et al, 1999;Wang et al, 2001;Yi et al, 2001;Hibi et al, 2002;Nakayama et al, 2002;van Rijnsoever et al, 2002;Zou et al, 2002;Cheng et al, 2003;Norrie et al, 2003;Yang et al, 2003;Sanz-Casla et al, 2005;Iacopetta et al, 2006;Ishiguro et al, 2006;Li et al, 2006;Miranda et al, 2006;Krtolica et al, 2007;Mas et al, 2007;Nakayama et al, 2007;Kazama et al, 2008;Wettergren et al, 2008;Goto et al, 2009;Aoyagi et al, 2011;Miladi-Abdennadher et al, 2011;Iida et al, 2012;Sameer et al, 2012;Veganzones-de-Castro et al, 2012). The publication years of the eligible studies ranged from 1999 to 2012.…”

Section: Characteristics Of Included Studiesmentioning

confidence: 98%

Relationships Betweenp16Gene Promoter Methylation and Clinicopathologic Features of Colorectal Cancer: A Meta-Analysis of 27 Cohort Studies

Chen

Liú²,

Zhao³

et al. 2014

DNA and Cell Biology

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Many existing studies have demonstrated that p16 promoter methylation might be correlated with the clinicopathologic features of colorectal cancer (CRC), but individually published results are inconclusive. This meta-analysis aimed to derive a more precise estimation of the relationships between p16 promoter methylation and the clinicopathologic features of CRC. We searched the CISCOM, CINAHL, Web of Science, PubMed, Google Scholar, EBSCO, Cochrane Library, and CBM databases from inception through August 1, 2013. Meta-analysis was performed using the STATA 12.0 software. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated under fixed- or random-effects models. Twenty-seven clinical cohort studies were included with a total of 3311 CRC patients. Our meta-analysis results revealed that p16 promoter methylation was associated with pathological characteristics of CRC (tumor, nodes, metastasis stage: OR=1.55, 95% CI: 1.14-2.13, p=0.006; lymph node metastasis: OR=2.40, 95% CI: 1.37-4.19, p=0.002; histologic grade: OR=2.72, 95% CI: 1.63-4.54, p<0.001; Dukes stage: OR=2.06, 95% CI: 1.57-2.71, p=0.002; tumor size: OR=1.99, 95% CI: 1.03-3.85, p=0.041; location: OR=2.49, 95% CI: 1.95-3.18, p<0.001, respectively). Subgroup analysis by ethnicity suggested that there were also significant correlations between p16 gene promoter methylation and pathological characteristics of CRC among both Caucasian and Asian populations (all p<0.05). Our meta-analysis suggests that promoter methylation of the p16 gene may be strongly correlated with the clinicopathologic features of CRC. Thus, p16 gene promoter methylation may be a potential biomarker for CRC.

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“…Similarly to the present study, CDKN2A methylation was observed in 24.8% of CRC specimens of Spanish CRC patients. 33 Despite some reports suggesting that CDKN2A methylation might be associated with good prognosis, 33 recent largescale studies and a literature meta-analysis revealed that it is a marker of poor prognosis in CRC and other surgically treated cancers. 32,34 RASSF1A methylation levels have been also largely investigated in CRC tissues.…”

Section: Discussionmentioning

confidence: 99%

Gene promoter methylation in colorectal cancer and healthy adjacent mucosa specimens

et al. 2014

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p16 gene methylation in colorectal cancer patients with long-term follow-up

Cited by 25 publications

References 37 publications

Prognostic factors in oral and oropharyngeal cancer based on ultrastructural analysis and DNA methylation of the tumor and surgical margin

Prognostic factors in oral and oropharyngeal cancer based on ultrastructural analysis and DNA methylation of the tumor and surgical margin

Relationships Betweenp16Gene Promoter Methylation and Clinicopathologic Features of Colorectal Cancer: A Meta-Analysis of 27 Cohort Studies

Gene promoter methylation in colorectal cancer and healthy adjacent mucosa specimens

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