P16-positive secondary tongue squamous cell carcinoma following allogeneic hematopoietic stem cell transplantation: A case report and literature review

Wu, Tiantian; Liu, Zhenmin; Yong, Xiangzhi; Mei, Guocheng; Jiang, Qiaozhi; Fang, Meifei; Li, Ping; Zhang, Zhongming; Tao, Renchuan

doi:10.1016/j.oraloncology.2021.105399

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…9 Furthermore, oral cGVHD poses a risk for the development of oral squamous cell carcinomas (OSCCs). 10,11 Given the adverse effects of oral cGVHD on both oral and systemic health, raising awareness is crucial for understanding its occurrence and progression.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

Yong,

Zhou,

et al. 2024

Adv Clin Exp Med

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Background. Oral chronic graft-versus-host disease (cGVHD) impacts quality of life of patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, its precise pathogenesis remains unknown, with potential associations with differential microRNA (miRNA) expression and the TGF-β/Smad signaling pathway.Objectives. This study aims to explore miRNA expression profiles in the peripheral blood of oral cGVHD patients, focusing on miRNA-769-5p and its relationship with Smad2. Materials and methods.Peripheral venous blood samples were collected for RNA extraction from 8 patients with oral cGVHD, 8 patients without cGVHD and 8 participants from the healthy control group. The miRNA library was constructed using the Illumina Hiseq 2500 platform. We focused on identifying miRNAs associated with the TGF-β/Smad signaling pathway and subsequently conducted validation experiments. The oral cGVHD and without cGVHD groups were each expanded to include 15 individuals. Peripheral blood samples were subjected to polymerase chain reaction (PCR) analysis to assess miRNA levels and to evaluate Smad2 mRNA levels in peripheral blood mononuclear cells (PBMC). Additionally, enzyme-linked immunosorbent assay (ELISA) was conducted to determine the Smad2 protein levels in peripheral blood.Results. The most significantly differentially expressed miRNAs among the 3 groups were miRNA-505-5p and miRNA-769-5p. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated an enrichment of the target genes of miRNA-769-5p in the TGF-β signaling pathway. It was observed that miRNA-769-5p expression was higher in patients without oral cGVHD in comparison to those with oral cGVHD. Receiver operating characteristic (ROC) analysis demonstrated that miRNA-769-5p holds diagnostic value for oral cGVHD. As a target of miRNA-769-5p, Smad2 mRNA exhibited a negative correlation with it. Moreover, both Smad2 mRNA and protein levels were higher in patients with oral cGVHD as opposed to those without cGVHD.Conclusions. Differential expression of miRNAs, particularly the downregulation of miRNA-769-5p, may influence the development of oral cGVHD by diminishing its inhibitory effect on the TGF-β/Smad signaling pathway through its interaction with Smad2.

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Section: Introductionmentioning

confidence: 99%

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

Yong,

Zhou,

et al. 2024

Adv Clin Exp Med

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“…Besides, oral cGVHD is a risk for the development of oral squamous cell carcinomas [9,10]. Given the deleterious impact on oral even system health caused by oral cGVHD, consciousness is needed in the occurrence and progress of oral cGVHD.…”

Section: Introductionmentioning

confidence: 99%

miRNA-769-5p and Smad2 Differential Expression in Patients with or without Oral cGVHD

Yong

Zhou

Jiang

et al. 2023

Preprint

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Background: Oral chronic graft versus host disease (Oral cGVHD) affects the life quality of patient. But its pathogenesis is still unclear. MicroRNA (miRNA) involves in numerous pathologic and physiologic processes. Objective: To preliminarily observe the change of miRNAs in oral cGVHD. Methods: Peripheral venous blood was taken from 8 cases of oral cGVHD, 8 cases without oral cGVHD, and 8 cases of healthy control group to construct the miRNAs library by Illumina Hiseq 2500 platform. The expression of miRNA-769-5p was detected by qRT-PCR in each group with subjects adding to 15. The interactions between miRNA-769-5p and Smad2 mRNA was verified by dual-luciferase assay. qRT-PCR and ELISA was used to detect the mRNA and protein level of Smad2 in peripheral blood respectively. Results: All three groups expressed 518 miRNAs and 191 miRNAs showed significantly different expression. The expression of miRNA-769-5p was the higher in non-oral cGVHD patients than that in oral cGVHD patients, and were of certain value in the diagnosis of oral cGVHD. The levels of Smad2 mRNA and protein were higher in the oral cGVHD group than those without oral cGVHD. As a target of miRNA-769-5p, Smad2 mRNA was negatively related to it. Conclusion: Differential expression of miRNAs, especially miRNA-769-5p may be involved in oral cGVHD. High expression of circulating Smad2 may be associated with the oral cGVHD, which might be regulated by miRNA-769-5p.

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Oral microbial changes and oral disease management before and after the treatment of hematological malignancies: a narrative review

Lin

et al. 2023

Clin Oral Invest

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P16-positive secondary tongue squamous cell carcinoma following allogeneic hematopoietic stem cell transplantation: A case report and literature review

Cited by 3 publications

References 16 publications

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD

miRNA-769-5p and Smad2 Differential Expression in Patients with or without Oral cGVHD

Oral microbial changes and oral disease management before and after the treatment of hematological malignancies: a narrative review

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