P173 Coupled Nitroso-Sulfide Signalization Triggers Specific Vasoactive Effects in Intrarenal Arteries of Patients With Arterial Hypertension

Čačányiová, Soňa; Berenyiová, Andrea; Kristek, Frantisek; Grman, Marian; Ondriaš, Karol; Breza, J

doi:10.1016/j.artres.2017.10.121

Cited by 10 publications

(17 citation statements)

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“…According to these findings, HNO very probably does not represent the crucial vasorelaxant compound of the H 2 S/GSNO mixture. These results correlate with our previous results, when we showed that the H 2 S/GSNO products evoked similar vasoactive effect with similar mechanisms in the lobar artery isolated from hypertensive patient as well as of the renal artery isolated from spontaneously hypertensive rats [33].…”

Section: Discussionsupporting

confidence: 92%

“…It has been reported that H 2 S potentiates the release of NO from GSNO in a pH-dependent fashion; therefore, we could assume that H 2 S may be the compound responsible for the extent of GSNO relaxation after incubation with garlic juice [16]. Evidence of the H 2 S/GSNO interaction has also been demonstrated by several other studies [16,21,33]. Studies on adult normotensive rats suggested that NO released from GSNO may interact with H 2 S to form an unknown complex directly in arterial tissue because preincubation of the rat thoracic aorta with a low concentration of H 2 S subsequently led to heightened GSNO-induced relaxation even after elimination of H 2 S from the organ bath.…”

Section: Discussionsupporting

confidence: 76%

“…Several compounds have been suggested to play a role in mediating the interaction between S-nitrosothiols (RSNO) and H 2 S, altering their bioactive effects: nitroxyl (HNO), thionitrous acid (HSNO), nitrosopersulfide (SSNO -), N-nitrosohydroxylamine-N-sulfonate (SULFI/NO) and polysulfides (HSx -) [17][18][19][20][21]36]. Moreover, Cacanyiova et al [33] studied the renal arteries of spontaneously hypertensive rats and found that the production of specific reaction products was coupled with a unique interaction between GSNO and H 2 S, as using a different chemical NO donor (DEA NONOate) in the mixture with an H 2 S donor behaved differently than application of GSNO. Cortese-Krott et al [16] proposed SSNOto be a major longer-lived product of the sulfide/RSNOs reaction.…”

Section: Discussionmentioning

confidence: 99%

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The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice

et al. 2020

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The beneficial cardiovascular effects of garlic have been reported in numerous studies. The major bioactive properties of garlic are related to organic sulfides. This study aimed to investigate whether garlic juice works exclusively due to its sulfur compounds or rather via the formation of new products of the nitroso-sulfide signaling pathway. Changes in isometric tension were measured on the precontracted aortic rings of adult normotensive Wistar rats. We evaluated NO-donor (S-nitrosoglutathione, GSNO)-induced vasorelaxation and compare it with effects of hydrogen sulfide (H2S)/GSNO and garlic/GSNO. Incubation with garlic juice increased the maximal GSNO-induced relaxation and markedly changed the character of the relaxant response. Although incubation with an H2S donor enhanced the maximal vasorelaxant response of GSNO, neither the absolute nor the relative relaxation changed over time. The mixture of GSNO with an H2S donor evoked a response similar to GSNO-induced relaxation after incubation with garlic juice. This relaxation of the H2S and GSNO mixture was soluble guanylyl cyclase (sGC) dependent, partially reduced by HNO scavenger and it was adenosine triphosphate-sensitive potassium channels (KATP) independent. In this study, we demonstrate for the first time the suggestion that H2S itself is probably not the crucial bioactive compound of garlic juice but rather potentiates the production of new signaling molecules during the GSNO-H2S interaction.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice

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“…They proposed that S-nitrosothiols are transient intermediates that react with thiols to form stable persulfides [72]. The direct interaction between H 2 S and NO results in the formation of biologically active nitrosopersulfide and polysulfides [73][74][75][76]. In addition, H 2 S may interact with other reactive species, e.g., oxygen, nitrogen, sulfur and selenium species.…”

Section: H 2 S Signalingmentioning

confidence: 99%

Hydrogen Sulfide in Pharmacotherapy, Beyond the Hydrogen Sulfide-Donors

et al. 2020

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Hydrogen sulfide (H2S) is one of the important biological mediators involved in physiological and pathological processes in mammals. Recently developed H2S donors show promising effects against several pathological processes in preclinical and early clinical studies. For example, H2S donors have been found to be effective in the prevention of gastrointestinal ulcers during anti-inflammatory treatment. Notably, there are well-established medicines used for the treatment of a variety of diseases, whose chemical structure contains sulfur moieties and may release H2S. Hence, the therapeutic effect of these drugs may be partly the result of the release of H2S occurring during drug metabolism and/or the effect of these drugs on the production of endogenous hydrogen sulfide. In this work, we review data regarding sulfur drugs commonly used in clinical practice that can support the hypothesis about H2S-dependent pharmacotherapeutic effects of these drugs.

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“…Exogenously added and endogenously produced hydrogen sulfide (H 2 S) and polysulfides affect many physiological and pathologic processes [ 1 , 2 , 3 , 4 ]. They modulate oxidative stress by reacting with reactive oxygen and nitrogen species [ 1 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

•BMPO-OOH Spin-Adduct as a Model for Study of Decomposition of Organic Hydroperoxides and the Effects of Sulfide/Selenite Derivatives. An EPR Spin-Trapping Approach

et al. 2020

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Lipid hydroperoxides play an important role in various pathophysiological processes. Therefore, a simple model for organic hydroperoxides could be helpful to monitor the biologic effects of endogenous and exogenous compounds. The electron paramagnetic resonance (EPR) spin-trapping technique is a useful method to study superoxide (O2•−) and hydroxyl radicals. The aim of our work was to use EPR with the spin trap 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), which, by trapping O2•− produces relatively stable •BMPO-OOH spin-adduct, a valuable model for organic hydroperoxides. We used this experimental setup to investigate the effects of selected sulfur/selenium compounds on •BMPO-OOH and to evaluate the antioxidant potential of these compounds. Second, using the simulation of time-dependent individual BMPO adducts in the experimental EPR spectra, the ratio of •BMPO-OH/•BMPO-OOH—which is proportional to the transformation/decomposition of •BMPO-OOH—was evaluated. The order of potency of the studied compounds to alter •BMPO-OOH concentration estimated from the time-dependent •BMPO-OH/•BMPO-OOH ratio was as follows: Na2S4 > Na2S4/SeO32− > H2S/SeO32− > Na2S2 ~Na2S2/SeO32− ~H2S > SeO32− ~SeO42− ~control. In conclusion, the presented approach of the EPR measurement of the time-dependent ratio of •BMPO-OH/•BMPO-OOH could be useful to study the impact of compounds to influence the transformation of •BMPO-OOH.

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P173 Coupled Nitroso-Sulfide Signalization Triggers Specific Vasoactive Effects in Intrarenal Arteries of Patients With Arterial Hypertension

Cited by 10 publications

References 0 publications

The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice

The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice

Hydrogen Sulfide in Pharmacotherapy, Beyond the Hydrogen Sulfide-Donors

•BMPO-OOH Spin-Adduct as a Model for Study of Decomposition of Organic Hydroperoxides and the Effects of Sulfide/Selenite Derivatives. An EPR Spin-Trapping Approach

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