P2 receptors in satellite glial cells in trigeminal ganglia of mice

Weick, Michael; Cherkas, Pavel; Härtig, Wolfgang; Pannicke, Thomas; Uckermann, Ortrud; Bringmann, Andreas; Tal, Michael; Reichenbach, Andreas; Hanani, Menachem

doi:10.1016/s0306-4522(03)00388-9

Cited by 100 publications

(101 citation statements)

References 41 publications

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“…SGCs in SCG may be a target for ATP. Purinergic signaling is a major mode of neuron-glial communication [11,45,53,60]. GS expression in SCG after MI injury was increased.…”

Section: Discussionmentioning

confidence: 99%

Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor

Peng

et al. 2013

Purinergic Signalling

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P2X receptors participate in cardiovascular regulation and disease. After myocardial ischemic injury, sensorysympathetic coupling between rat cervical DRG nerves and superior cervical ganglia (SCG) facilitated sympathoexcitatory action via P2X 7 receptor. The results showed that after myocardial ischemic injury, the systolic blood pressure, heart rate, serum cardiac enzymes, IL-6, and TNF-α were increased, while the levels of P2X 7 mRNA and protein in SCG were also upregulated. However, these alterations diminished after treatment of myocardial ischemic (MI) rats with the P2X 7 antagonist oxATP. After siRNA P2X 7 in MI rats, the systolic blood pressure, heart rate, serum cardiac enzymes, the expression levels of the satellite glial cell (SGC) or P2X 7 were significantly lower than those in MI group. The phosphorylation of ERK 1/2 in SCG participated in the molecular mechanism of the sympathoexcitatory action induced by the myocardial ischemic injury. Retrograde tracing test revealed the sprouting of CGRP or SP sensory nerves (the markers of sensory afferent fibers) from DRG to SCG neurons. The upregulated P2X 7 receptor promoted the activation of SGCs in SCG, resulting in the formation of sensory-sympathetic coupling which facilitated the sympathoexcitatory action. P2X 7 antagonist oxATP could inhibit the activation of SGCs and interrupt the formation of sensory-sympathetic coupling in SCG after the myocardial ischemic injury. Our findings may benefit the treatment of coronary heart disease and other cardiovascular diseases.

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“…SGCs in SCG may be a target for ATP. Purinergic signaling is a major mode of neuron-glial communication [11,45,53,60]. GS expression in SCG after MI injury was increased.…”

Section: Discussionmentioning

confidence: 99%

Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor

Peng

et al. 2013

Purinergic Signalling

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“…Therefore acutely isolated cells are preferred, but so far very little work has been done on cultured SGCs. We used this method for patch clamp recordings from SGCs in mouse trigeminal ganglia [182]. Culturing intact ganglia (organ culture) is a useful model, as the relations among cells appears to be preserved over periods of months [22], and therefore this method might offer advantages for investigating SGCs.…”

Section: Overview Of Sensory Gangliamentioning

confidence: 99%

“…Isolated intact sensory ganglia should be the first choice for studying SGCneuron interactions as there is minimal tissue disruption. We made patch clamp recordings and measured in intact Ca 2+ levels in intact mouse trigeminal ganglia [26,182]. The thickness of intact ganglia might interfere with optical recordings, but using confocal microscopy can largely overcome this problem.…”

Section: Overview Of Sensory Gangliamentioning

confidence: 99%

Satellite glial cells in sensory ganglia: from form to function

Hanani

2005

Brain Research Reviews

648

744

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Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

“…only 0.5% in the mouse (Ruan et al, 2004), less than 1% in the rat (Bradbury et al, 1998) and 1.7% in the cat . Interactions may also occur between trigeminal ganglion neurons and satellite glial cells that involve P2 receptors including P2X and P2Y receptors (Ruan and Burnstock, 2003;Weick et al, 2003).This is the first study to quantitatively examine P2X 2 receptor expression in the rat trigeminal ganglion, and examine colocalisation with NF200 and IB4. P2X 2 receptors have previously been reported in trigeminal ganglia (Xiang et al, 1998) and specifically on trigeminal sensory neurons innervating the masseter muscle (Ambalavanar et al, 2005).…”

mentioning

confidence: 91%

Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons

et al. 2007

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Purine receptors have been implicated in central neurotransmission from nociceptive primary afferent neurons, and adenosine 5'tri-phosphate (ATP)-mediated currents in sensory neurons have been shown to be mediated by both P2X 3 and P2X 2/3 receptors. The aim of the present study was to quantitatively examine the distribution of P2X 2 and P2X 3 receptors in primary afferent cell bodies in the rat trigeminal ganglion, including those innervating the dura. In order to determine the classes of neurons that express these receptor subtypes, purine receptor immunoreactivity was examined for colocalisation with markers of myelinated (neurofilament 200; NF200) or mostly unmyelinated, nonpeptidergic fibres (Bandeiraea simplicifolia isolectin B4; IB4). 40 % of P2X 2 and 64 % of P2X 3 receptor-expressing cells were IB4 positive, and 33 % of P2X 2 and 31 % of P2X 3 receptor-expressing cells were NF200 positive. Approximately 40 % of cells expressing P2X 2 receptors also expressed P2X 3 receptors and vice versa. Trigeminal ganglion neurons innervating the dura mater were retrogradely labelled and 52 % of these neurons expressed either P2X 2 or P2X 3 or both receptors. These results are consistent with electrophysiological findings that P2X receptors exist on the central terminals of trigeminal afferent neurons, and provide evidence that afferents supplying the dura express both receptors. In addition, the data suggest specific differences exist in P2X receptor expression between the spinal and trigeminal nociceptive systems. Keywords purine receptors; trigeminal ganglia; sensory neuron; migraine; pain Purine receptors have been widely implicated in nociceptive processing (Burnstock, 2000;Khakh, 2001;Liu and Salter, 2005). The receptors are of two types: ionotropic (P2X) and metabotropic (P2Y), both of which are stimulated by adenosine 5'tri-phosphate (ATP). Seven P2X receptor subtypes have been cloned, and some occur as heteromultimers (e.g. P2X 2/3 , P2X 1/5 and P2X 4/6 receptors). In the spinal somatosensory system, all P2X receptor subtypes with the exception of P2X 7 are expressed in the spinal dorsal horn, the dorsal root ganglion (DRG), and the central terminals of primary afferent neurons (Burnstock, 2000;Khakh, 2001;North, 2002). In vivo and in vitro electrophysiological recordings from single dorsal horn neurons have implicated P2X receptors on primary afferent terminals in spinal nociceptive transmission (Nakatsuka and Gu, 2001;Nakatsuka et al., 2002;Nakatsuka et al., 2003; Correspondence details: Dr. Ernest Jennings Dept. Anatomy & Cell Biology The University of Melbourne Parkville, Victoria, 3010, Australia Tel: +61 3 8344 5802 Fax: +61 3 9347 9619 Email: e.jennings@unimelb.edu.au. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form...

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P2 receptors in satellite glial cells in trigeminal ganglia of mice

Cited by 100 publications

References 41 publications

Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor

Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor

Satellite glial cells in sensory ganglia: from form to function

Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons

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