p21 participates in the regulation of anaplastic thyroid cancer cell proliferation by miR-146b

Abstract

Abstract. Anaplastic thyroid carcinoma (ATC) originates from completely undifferentiated cells, and is the most lethal type of thyroid-derived tumor. Numerous microRNAs have significant roles in tumorigenesis by targeting relevant genes. The role of microRNA 146b (miR-146b) in ATC remains to be elucidated. In order to characterize the role of miR-146b in ATC, overexpression or interference of miR-146b was induced in ATC cell lines, and cell proliferation and migration were evaluated. The potential targets of miR-146b were searched in the Gene Expression Omnibus database for ATC and matched non-tumor control samples. The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines. In the present study, cell proliferation was promoted when overexpression of miR-146b was induced in ATC, and inhibited when interference of miR-146b was induced, which indicated that miR-146b affects the proliferation of ATC cells in vitro. In addition, cell migration of ATC was also affected by miR-146b. During the search for potential targets of miR-146b in ATC, p21 (also known as p21 Waf1/Cip1 or CDKN1A) was noted for its role in cell cycle progression and tumor pathogenesis. The expression level of p21 was influenced by the level of miR-146b, and the results of the present study demonstrated that the level of p21 was increased when FRO cells were transformed with miR-146b mimic, and p21 was downregulated when FRO cells transformed with anti-miR-146b. In conclusion, p21 may participate in the regulation of ATC cell proliferation by miR-146b.

MicroRNA-146b: A Novel Biomarker and Therapeutic Target for Human Papillary Thyroid Cancer

“…Riesco-Eizaguirre et al [39] stated that miR-146b binds to the 3′-untranslated region of PAX8 and NIS , thus repressing iodide protein translation and leading to insensitivity to radioactive iodide treatment. Wang et al [40] reported that p21 is regulated by miR-146b and leads to tumor proliferation and migration in anaplastic thyroid cancer (ATC) cells. However, there are conflicting reports for miR-146b in other solid neoplasms.…”

C5aR deficiency attenuates the breast cancer development via the p38/p21 axis

“…P21 is negatively associated with the cancer occurrence [ 34 , 35 ]. Consistent with these results, we observed that the p21 expression was reduced in the tumor tissues and its low expression was closely associated with poor survival rates in BC patients.…”

Poorly Differentiated and Anaplastic Thyroid Cancer: Insights into Genomics, Microenvironment and New Drugs

“…According to literature data, we grouped them into 3 main roles: regulation of growth tumor, invasiveness and resistance to therapy (Figure 2). We found that 9 miRNA were related to tumor growth [52][53][54][55][56][57][58][59][60][61], 14 to tumor growth and invasiveness [62][63][64][65][66][67][68][69], 28 to invasiveness [67,[70][71][72][73][74][75][76], and 66 to therapies resistance (62 to anti-BRAF treatment, 3 to chemotherapy and 1 to radiotherapy) [77][78][79][80][81]. Additionally, 10 miRNAs were considered as an ancillary diagnostic tool [82,83] (Figure 2).…”