P272 Treatment of menopausal syndrome by beevenom and bee propolis (a new modality)

Farid, Asim; Bambi, M El; Dali, O El; Essia, S

doi:10.1016/s0378-5122(97)81465-x

Cited by 3 publications

(2 citation statements)

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“…Vaginal epithelial proliferation and cornification observed with EEP treatment are desired estrogenic effects, because the lactobacillus use these superficial cells to produce lactic acid, which keeps the vaginal milieu acidic and thus prevent ascending infections [ 50 ]; Moreover, vaginal secretive cells will keep the vagina wet, thereby avoiding vaginal dryness. The Combination of therapy with bee venom (0.2 mg) and bee propolis (0.5 g) was shown to alleviate hot flushes in 85% of treated menopausal women [ 51 ]. These observations concord with part of results obtained with EEP (150 mg/kg) in hot flushes induced in rats.…”

Section: Discussionmentioning

confidence: 99%

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Zingue

Nde

Michel

et al. 2017

BMC Complement Altern Med

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BackgroundSince the biological properties of propolis depend to the plants that can be found in a specific region, propolis from unexplored regions attracts the attention of scientists. Ethanolic extract of Cameroonian propolis (EEP) is used to treat various ailments including gynecological problems and amenorrhea. Since there were no scientific data to support the above claims, the present study was therefore undertaken to assess estrogenic properties of Cameroonian propolis.MethodsTo achieve our goal, the ability of EEP to induce MCF-7 cells proliferation in E-screen assay as well as to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney cells (HEK293T) transfected with ERs was tested. Further, a 3-day uterotrophic assay was performed and the ability of EEP to alleviate hot flushes in ovariectomized adult rats was evaluated.ResultsIn vitro, EEP showed an antiestrogenic activity in both HEK293T ER-α and ER-β cells. In vivo, EEP induced a significant increase in a bell shape dose response manner of the uterine wet weight, the total protein levels in the uterus, the uterine and vaginal epithelium height and acini border cells of mammary gland with the presence of abundant eosinophil secretions. Moreover, EEP induced a significant decrease in the total number, average duration as well as frequency of hot flushes after 3 days of treatment in rat (equivalent to a month in woman). The dose of 150 mg/kg exhibited the most potent estrogenic effects among all the tested doses. The UPLC-HRMS analysis showed the presence of caffeic acid derivatives and trirtepernoids in EEP, which are well known endowed with estrogenic properties.ConclusionThese results suggest that Ethanolic extract of Cameroonian propolis has estrogen-like effects in vivo and may alleviate some menopausal problems such as vaginal dryness and hot flushes.Graphical abstractEthanol-extracted Cameroobian propolis exhibited in vitro and in vivo estrogen-like effects. This extract may contain promising phytoestrogens.

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Section: Discussionmentioning

confidence: 99%

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Zingue

Nde

Michel

et al. 2017

BMC Complement Altern Med

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“…CAPE also inhibited phorbol ester-induced H 2 O 2 production and tumor promotion Frenkel et al, 1993). Propolis has been used for the treatment of breast tumors in Oriental medicine, and combination therapy with bee venom (0.2 mg) and bee propolis (0.5 g) was shown to alleviate menopausal syndrome in 85% of treated menopausal women (Farid et al, 1997). Although propolis has been used for the treatment of breast tumors and menopausal women, virtually no studies have been performed on the estrogenic effects of propolis.…”

Section: Introductionmentioning

confidence: 99%

Caffeic acid phenethyl ester, a component of beehive propolis, is a novel selective estrogen receptor modulator

Jung

Kim

et al. 2009

Phytotherapy Research

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Caffeic acid phenethyl ester (CAPE) is an active ingredient of beehive propolis with a structure similar to phenolic acid. The estrogenic effects of propolis were previously demonstrated through the activation of an estrogen receptor. To identify the estrogenic properties of propolis, CAPE was evaluated using in vitro and in vivo methods. CAPE showed selective binding affi nity to human estrogen receptor b (hERb) rather than hERa. CAPE also reduced ERa expression in MCF-7 and MDA 231 cells. In the yeast estrogen receptor transcription assay, CAPE produced the transcriptional activity of estrogen-responsive element with EC 50 values of 3.72 × 10 −6 M. CAPE did not increase the growth of MCF-7 estrogen receptor-positive breast cancer cells in doses ranging from 10 −7 to 10 −5 M. In order to understand how CAPE acts in animals, CAPE was tested by a uterotrophic bioassay. Treatment with CAPE (100, 500 mg/kg) did not increase the uterine weight relative to 3 mg/kg 17b-estradiol treatment. The results indicate that CAPE, which is a selective agonist to hERb, but does not show any estrogenic effect on estrogen receptor-positive breast cancer cells and in immature rat uterine tissue, is a potential selective estrogen receptor modulator.

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Current Status of MHT Treatment for Menopausal Syndrome

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2023

ACM

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P272 Treatment of menopausal syndrome by beevenom and bee propolis (a new modality)

Cited by 3 publications

References 0 publications

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Caffeic acid phenethyl ester, a component of beehive propolis, is a novel selective estrogen receptor modulator

Current Status of MHT Treatment for Menopausal Syndrome

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