P35 and P22 Toxoplasma gondii antigens abbreviate regions to diagnose acquired toxoplasmosis during pregnancy: toward single-sample assays

Abstract

rP35a and rP22a features suggest that, with complementary use, they could replace parasite lysate for toxoplasmosis infection screening and for acute toxoplasmosis diagnosis. Our proposal should be validated by a longitudinal study and may lead to a reliable toxoplasmosis pregnancy control, performing tests in only one serum sample.

The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera

“…gondii infection has not been found so far, it is necessary to determine the dynamic of maturation of IgG antibodies. To date, only a few papers can be found in the literature regarding the use of recombinant proteins to determine the avidity of IgG antibodies [33,43,44,53]. We believe this is mainly due to difficult interpretation of results from such experiments, based on a number of assumptions that must be made at the beginning of the assay.…”

“…Although every commercially available test contains a description of how the results should be interpreted, some works presuppose that borderline avidity for recombinant proteins should be in between 0.3–0.4 [33,42,43]. However, in view of the results presented by Costa et al, [44], it seems that determination of borderline avidity is dependent on the specificity of antigen used in the test. In our work, we described both ways of interpreting results from avidity assessment.…”

“…Regardless of the interpretation method, the results allowed us to draw similar conclusions, though determination of the IgG avidity index based on ROC analysis was more reliable. The model proposed by Costa et al, [44], includes the possibility of a diversified response to specific antigens against which anti- T . gondii antibodies are directed.…”

“…This classification was established on the basis of the results obtained and applied previously [33,42,43]. In addition, ROC analysis was performed, on the basis of which a low, borderline, and high avidity indexes were determined using the method described previously [44].…”

Toxoplasma gondii Recombinant antigen AMA1: Diagnostic Utility of Protein Fragments for the Detection of IgG and IgM Antibodies

“…These serological tests are based on single recombinant proteins, protein mixtures and, more recently, newer tools, such as chimeric antigens. Furthermore, the diagnostic usefulness of some preparations of recombinant antigens in determining IgG avidity assays has been estimated [6,[11][12][13][14][15][16][17]. Despite the very promising results described in numerous studies, new antigenic proteins of T. gondii with potential diagnostic utility are still being researched and tested.…”