p53 polymorphism in codon 72 and risk of human papillomavirus-induced cervical cancer: effect of inter-laboratory variation

Makni, Héla; Franco, Eduardo L.; Kaiano, Jane Haruko Lima; Villa, Luisa L.; Labrecque, Sylvie; Dudley, Roy; Storey, Alan; Matlashewski, Greg

doi:10.1002/1097-0215(20000815)87:4<528::aid-ijc11>3.0.co;2-o

Cited by 86 publications

(30 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are, in addition, several other factors which may have contributed to a lack of conformity in results obtained in different studies. For example, interlaboratory variation in protocols for the analysis of codon 72 status could result in misclassification (25) while methodology for the detection of HPV DNA also varies widely. In our study the 98% incidence of HPV‐positive tumors accords with recent suggestions that HPV is universally present in cervical tumors (26) .…”

Section: Discussionmentioning

confidence: 99%

P53 codon 72 polymorphism and human papillomavirus type in relation to cervical cancer in South African women

Pegoraro

Rom²,

Lanning³

et al. 2002

Int J Gynecol Cancer

View full text Add to dashboard Cite

The usefulness of the arginine (Arg) residue at codon 72 of the p53 tumor suppressor gene as a marker for the risk of cervical cancer remains unclear. Studies to date have focused mainly on Caucasian subjects despite marked ethnic variations in both the p53 polymorphism and the frequency of cervical carcinoma. Furthermore, not all studies have taken into account the type of human papillomavirus (HPV) infection present. In this study, undertaken at King Edward VIII Hospital, Durban, South Africa, we determined the p53 codon 72 status in 281 black South African women with cervical cancer and 340 ethnically matched healthy control subjects. In addition, HPV DNA was confirmed in 190 cervical tumors and the viral type determined. Results showed that overall more cancer patients than control subjects had an Arg allele at codon 72 with respect to both genotype and allelotype (P < 0.05). A significantly higher (P < 0.001) Arg allele frequency (55%) was also observed in patients whose tumors contained low or intermediate risk HPV DNA compared with control subjects (31%); the Arg homozygosity rate was 34% and 9% in patients and controls, respectively (P < 0.001). In contrast, patients harboring HPV 16/18 infections showed no differences in p53 status compared with controls. It would appear that, in the absence of HPV 16/18 infections, the Arg allele at codon 72 of the p53 tumor suppressor gene may constitute a risk factor for carcinogenesis of the cervix.

show abstract

Section: Discussionmentioning

confidence: 99%

P53 codon 72 polymorphism and human papillomavirus type in relation to cervical cancer in South African women

Pegoraro

Rom²,

Lanning³

et al. 2002

Int J Gynecol Cancer

View full text Add to dashboard Cite

show abstract

“…Storey et al found an association between the majority allele, arginine (G) form of p53, and cervical cancer development and proposed that this genotype is more susceptible to HPV E6-mediated degradation [24]. Since then, there have been many reports on this TP53 polymorphism and risk for cervical cancer and the results are largely contradictory [25,26]. The frequency of TP53 codon 72 polymorphism and its relationship with HPV infection and cervical cancer is still unknown among Saudi women.…”

Section: Introductionmentioning

confidence: 99%

HPV prevalence and genetic predisposition to cervical cancer in Saudi Arabia

Alsbeih

Al-Harbi

El-Sebaie

et al. 2013

Infect Agents Cancer

View full text Add to dashboard Cite

BackgroundCervical cancer incidence is low in Saudi Arabian women, suggesting low prevalence to HPV infection due to environmental, cultural and genetic differences. Therefore, we investigated HPV prevalence and genotype distribution in cervical cancer as well as the association with 9 genetic single nucleotide polymorphisms (SNPs): CDKN1A (p21) C31A, TP53 C72G, ATM G1853A, HDM2 promoter T309G, HDM2 A110G, LIG4 A591G, XRCC1 G399A, XRCC3 C241T and TGFβ1 T10C, presumed to predispose to cancer.MethodsOne hundred cervical cancer patients (90 squamous cell carcinoma and 10 adenocarcinoma) and 100 age/sex-matched controls were enrolled. SNPs were genotyped by direct sequencing and HPV was detected and typed in tumors using the HPV Linear Array Test.ResultsEighty-two cases (82%) were positive for HPV sequences. Seven HPV genotypes were present as single infections (16, 18, 31, 45, 56, 59, 73) and five double infections (16/18, 16/39, 16/70, 35/52, 45/59) were detected. Most common genotypes were HPV-16 (71%), 31 (7%), and 18, 45, 73 (4% each). Only XRCC1 SNP was significantly associated with cervical cancer (P=0.02, OD=1.69; 95% CI= 1.06–2.66). However, nested analysis revealed a preponderance of HPV-positivity in patients harboring the presumed risk allele TP53 G (P=0.06). Both XRCC1 and TP53 SNPs tended to deviate from Hardy-Weinberg equilibrium (HWE; P=0.03-0.07).ConclusionsHPV prevalence (82%) in cervical cancer is at the lower range of the worldwide estimation (85 - 99%). While XRCC1 G399A was significantly associated with cervical cancer, TP53 G72C showed borderline association only in HPV-positive patients. Deviation from HWE in HPV-positive patients indicates co-selection, hence implicating the combination of HPV and SNPs in cancer predisposition. Thus, SNPs could be more relevant biomarkers of susceptibility to cervical cancer when associated with HPV infection.

show abstract

“…These characteristics could be responsible for the great variability of the frequencies reported, even within the same population [16]. In this sense, Makni [8] demonstrated a great inter-laboratory variation, however, after the exclusion of discordant genotypes an increasing association was found. DHPLC is a highly sensitive technique that detects sequence variations.…”

Section: Discussionmentioning

confidence: 96%

“…At present, several epidemiological studies on polymorphisms have shown controversial results, probably due to ethnical differences, inadequate selection of cases and controls, technical sensitivity and inter-laboratory variations [7,8]. Makni et al [8] reported that after the exclusion of discordant TP53 genotypes between laboratories, there was an increased association of the 72Arg/Arg genotype with CC and suggested that the misclassification consequent to inter-laboratory variation may affect the ability to detect the association.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphism in exon 4 of TP53 gene associated to HPV 16 and 18 in Mexican women with cervical cancer

Piña‐Sánchez¹,

Hernández-Hernández²,

Taja‐Chayeb³

et al. 2010

Med Oncol

View full text Add to dashboard Cite

Cervical cancer (CC) is the second most common cancer in Mexican women. Human papillomavirus (HPV) infection is necessary but not sufficient for CC development. Furthermore, genetic factors as polymorphisms could be important susceptibility factors. Controversial results regarding TP53 polymorphisms specifically in codon 72 of CC have been reported. In the present work, the exon 4 sequence of TP53 gene in CC and healthy Mexican-mestizo women were analyzed. A group of 111 women with CC and 126 healthy women (control) were included. Peripheral blood cells for polymorphism analysis and cervical scrape for HPV detection were used. PCR of exon 4 of TP53 were subjected to denaturing high-performance liquid chromatography (DHPLC) analysis and sequencing. HPV detection was subjected to PCR and sequencing. The statistical analysis was carried out using the Arlequin software. Codon 72 Arg/Arg was the most common SNP detected, and Hardy-Weinberg analysis showed equilibrium in control and CC samples (P>0.05). Wild type sequence of TP53 exon 4 was detected in 66 and 57% in control and CC samples, respectively. For codon 72 Arg/Arg, differences between control and CC women were found (P=0.043). An association between HPV 16/18 infection and 72 Arg/Arg in woman with CC was found (P=0.026). Haplotype GC (codon 36 and 72) was statistically significantly associated with CC (P=0.011). HPV 16 was the most common viral type. Codon 72 Arg/Arg is the most common polymorphism in the Mexican population and could be associated to HPV 16 and/or HPV 18 infection in CC.

show abstract

p53 polymorphism in codon 72 and risk of human papillomavirus-induced cervical cancer: effect of inter-laboratory variation

Cited by 86 publications

References 29 publications

P53 codon 72 polymorphism and human papillomavirus type in relation to cervical cancer in South African women

P53 codon 72 polymorphism and human papillomavirus type in relation to cervical cancer in South African women

HPV prevalence and genetic predisposition to cervical cancer in Saudi Arabia

Polymorphism in exon 4 of TP53 gene associated to HPV 16 and 18 in Mexican women with cervical cancer

Contact Info

Product

Resources

About