p75 neurotrophin receptor regulates NGF-induced myofibroblast differentiation and collagen synthesis through MRTF-A

Liu, Zhenxing; Cao, Yongqian; Liu, Guijun; Yin, Shuai; Ma, Jiaxu; Liu, Jian; Zhang, Min; Wang, Yibing

doi:10.1016/j.yexcr.2019.111504

Cited by 24 publications

(18 citation statements)

References 40 publications

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“…During muscle development or regeneration, myocytes transiently produce NGF, as well as its tyrosine-kinase and p75 receptors, but when myoblasts were screened for the expression of NGF receptors, only p75NTR was detected, while the highaffinity NGF receptor, TrkA, was not present [57]. Recent studies have suggested that NGF stimulates myoblast differentiation and collagen synthesis, but the regulatory mechanism remains poorly defined [58]. In addition, it has been reported that the p75NTR receptor could represent a key regulator of the NGF-mediated myoprotective effect on satellite cells, but the precise function of the NGF/p75 signaling pathway in myogenic cell proliferation, survival and differentiation remains fragmented and controversial [59].…”

Section: Ngfmentioning

confidence: 99%

Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging

Mancinelli

Checcaglini

Coscia

et al. 2021

IJMS

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In the last decade, clear evidence has emerged that the cellular components of skeletal muscle are important sites for the release of proteins and peptides called “myokines”, suggesting that skeletal muscle plays the role of a secretory organ. After their secretion by muscles, these factors serve many biological functions, including the exertion of complex autocrine, paracrine and/or endocrine effects. In sum, myokines affect complex multi-organ processes, such as skeletal muscle trophism, metabolism, angiogenesis and immunological response to different physiological (physical activity, aging, etc.) or pathological states (cachexia, dysmetabolic conditions, chronic inflammation, etc.). The aim of this review is to describe in detail a number of myokines that are, to varying degrees, involved in skeletal muscle aging processes and belong to the group of proteins present in the functional environment surrounding the muscle cell known as the “Niche”. The particular myokines described are those that, acting both from within the cell and in an autocrine manner, have a defined relationship with the modulation of oxidative stress in muscle cells (mature or stem) involved in the regulatory (metabolic or regenerative) processes of muscle aging. Myostatin, IGF-1, NGF, S100 and irisin are examples of specific myokines that have peculiar features in their mechanisms of action. In particular, the potential role of one of the most recently characterized myokines—irisin, directly linked to an active lifestyle—in reducing if not reversing senescence-induced oxidative damage is discussed in terms of its possible application as an agent able to counteract the deleterious effects of muscle aging.

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Section: Ngfmentioning

confidence: 99%

Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging

Mancinelli

Checcaglini

Coscia

et al. 2021

IJMS

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“…This occurs through the Trk-Akt-cyclin D1 cascade reaction. Liu et al (2019) studied the role of p75NTR in NGF-induced myofibroblast differentiation and collagen synthesis by lentivirus transfection. The results further suggested that p75 NTR is preferentially expressed and sufficient to induce actin cytoskeletal remodeling, which is necessary for NGF-induced α-smooth muscle actin (α-SMA) expression.…”

Section: Discussionmentioning

confidence: 99%

Simulated microgravity significantly altered metabolism in epidermal stem cells

Chen

Jiang

et al. 2020

In Vitro Cell.Dev.Biol.-Animal

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Simulated microgravity can significantly affect various cell types and multiple systems of the human body, such as cardiovascular system, skeletal muscle system, and immune system, and is known to cause anemia and loss of electrolyte and fluids. Epidermal stem cells (EpSCs) were cultured in a rotary cell culture system (RCCS) bioreactor to simulate microgravity. The metabolites of EpSCs were identified by liquid chromatography-mass spectrometry (LC-MS). Compared with normal gravity (NG) group, a total of 57 different metabolites of EpSCs were identified (P < 0.05, VIP > 1), including lipids and lipid-like molecules (51 molecules), amino acids (5 molecules), nucleosides, nucleotides, and analogues (1 molecule). According to the partial least squares discriminant analysis (PLS-DA) score plot, a VIP > 1 and P < 0.05 were obtained for the 57 different metabolites, of which 23 molecules were significantly downregulated and 34 were significantly upregulated in simulated microgravity (SMG) group. These results showed that SMG has a significant impact on different pathways, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that multiple pathways were involved, mainly the amino acid metabolism pathway, lipid metabolism pathway, membrane transport pathway, and cell growth and death pathways. Thus, the metabolic profile of EpSCs was changed under SMG. Exploring the metabolic profile of EpSCs would be helpful to further understand the growth characteristics of EpSCs under SMG, which will provide a new approach to explore the metabolomics mechanism of stress injury and repair trauma under SMG.

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“…Animal and human clinical studies have found that MDSCs only differentiate unidirectionally in normal tissues, while in the injured tissues, its multipotency is activated and it can differentiate into a variety of cell types, contributing to the synchronous reconstruction of systemic or local blood vessels, muscle bers, and peripheral nerves [26][27][28] . A large number of studies [29][30][31][32] have demonstrated the injection of stem cell has the potential to restore the function of urethral sphincter in the treatment of SUI. Meanwhile, periurethral injection of MDSCs for the treatment of SUI have also been applied in clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Muscle-Derived Stem Cells Combined With Nerve Growth Factor Transplantation in the Treatment of Stress Urinary Incontinence in Rats

Fang¹,

Liu²,

Liu³

et al. 2020

Preprint

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Objective: To investigate the efficacy of muscle-derived stem cells (MDSCs) combined with nerve growth factor (NGF) in the treatment of stress urinary incontinence (SUI) in rats. Methods: MDSCs were isolated and extracted from 90 SD rats, and the stem cell characteristics of the cells were identified using flow cytometry. NGF overexpression plasmid was coated with adenovirus and qRT-PCR was applied to verify adenovirus transfection efficiency. The rat models of SUI were constructed and randomly divided into five groups: control group, PBS group, MDSCs+oe-NGF group, MDSCs+vector group, and MDSCs group. After 8 weeks of feeding, the effect of MDSCs combined with NGF on repairing SUI in rats was detected by measuring the leakage point pressure (LPP) rats and Masson staining of rat urethral sections. The expression of NGF and VEGF was detected by western blot and IHC staining. Results: Flow cytometry results showed that the isolated MDSCs had stem cell characteristics and good purity. NGF mRNA was significantly upregulated in MDSCs after transfection with adenovirus coated with NGF plasmid. Compared with the control group, the LPP and the ratio of muscle fibers/collagen fibers were significantly increased in the MDSCs treated groups, with the highest increase in the MDSCs + oe-NGF group. Western blot and IHC results showed that compared with the control group, the expression of NGF and VEGF in the urethral tissues in the MDSCs treated groups were significantly up-regulated, with the highest increase in the MDSCs + oe-NGF group. Conclusion: MDSCs alone can improve SUI in rats, while MDSCs combined with NGF is more effective in the treatment of SUI rats by up-regulating VEGF.

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p75 neurotrophin receptor regulates NGF-induced myofibroblast differentiation and collagen synthesis through MRTF-A

Cited by 24 publications

References 40 publications

Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging

Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging

Simulated microgravity significantly altered metabolism in epidermal stem cells

Muscle-Derived Stem Cells Combined With Nerve Growth Factor Transplantation in the Treatment of Stress Urinary Incontinence in Rats

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