PaCDPK1, a gene encoding calcium-dependent protein kinase from orchid, Phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge

Abstract

Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and characterization of an orchid, Phalaenopsis amabilis, CDPK gene, PaCDPK1, by using the rapid amplification of cDNA ends (RACE)-PCR technique. The full length cDNA of 2,310 bp contained an open reading frame for PaCDPK1 consisting of 593 amino acid residues. Sequence alignment indicated that PaCDPK1 shared similarities with other plant CDPKs. PaCDPK1 transcripts were expressed strongly in labellum but not in leaves and roots. In addition, the PaCDPK1 gene was transcriptionally activated in response to low temperature, wounding, and pathogen infection. To identify the regulatory role of the PaCDPK1 promoter, a construct containing the PaCDPK1 promoter fused to a beta-glucuronidase (GUS) gene was transferred into Arabidopsis by Agrobacterium-mediated transformation. GUS staining revealed that PaCDPK1/GUS expression was induced by cold, wounding, and pathogen challenge in leaves and stems of transgenic Arabidopsis. These results suggested that this PaCDPK1 gene promoter could be used as an endogenous promoter for biotechnological purposes in orchids.

PaPTP1, a Gene Encoding Protein Tyrosine Phosphatase from Orchid, Phalaenopsis amabilis, is Regulated During Floral Development and Induced by Wounding

“…The spatial expression of PaPTP1 in response to wounding was similar to that of PaCDPK1 in orchid plants . Previous studies have also demonstrated the differential spatial expression patterns following wound-induced transcriptional activation (Saravitz and Siedow 1996;Tsai et al 2007). It was found in soybean that by 48 h after wounding the transcript levels of lipoxygenase 8 decreased within the wounded section but increased markedly elsewhere within the wounded leaflet (Saravitz and Siedow 1996).…”

Expression Profiling of a Novel Calcium-Dependent Protein Kinase Gene,LeCPK2, from Tomato (Solanum lycopersicum) under Heat and Pathogen-Related Hormones

“…8,27) ET, JA, and SA are well documented as signal molecules playing important roles in the defense of plants against multiple pathogenic and insect attacks, [28][29][30][31][32][33] and also, CDPKs were reported to be involved in defense responses to various biotic stimuli. [34][35][36][37] Consequently, the question arises whether the responses of CDPK genes to pathogens and insect stimuli are induced by these pathogen-related phytohormones. Studies of CDPK genes in chili pepper (Capsicum annuum L.) provided clues to this question, CaCDPK3 and CaCDPK4 were found to be induced during pathogen infection in parallel with responses to ET, JA, and SA.…”

Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)

“…CDPKs have been demonstrated to be involved in regulating plant responses to a variety of stimuli, including hormones, cold/drought/salt stress, elicitor, light and sucrose (Urao et al 1994;Botella et al 1996;Sheen 1996;Frattini et al 1999;Patharkar and Cushman 2000;Saijo et al 2000;Romeis et al 2001;Murillo et al 2001;Yang and Komatsu 2001;Chico et al 2002;Martínez-Noël et al 2007;Tsai et al 2007;Wan et al 2007). In rice, transgenic plants with a disrupted SPK (a seed-specific CDPK) exhibited reduced accumulation of storage starch and proteins in immature seeds (Asano et al 2002).…”