Packing columns for capillary electrochromatography

Theory and Practice of UHPLC and UHPLC–MS

“…Usually a slurry solvent with low viscosity (i.e., acetone) is preferred for faster packing. Because of the fact that the slurry is a suspension, an ultrasonic bath or magnetic stirring can help to avoid agglomeration of particles in the reservoir, which can obstruct the packing (Colón et al, 2000).…”

“…Functional frits have to resist the high pressure applied and, at the same time, allow the crossing of mobile phase and analytes through the column (Colón et al, 2000). Consequently frit fabrication represents a big challenge for the final performance of the column.…”

“…Also the reproducibility of frits is problematic, and special attention has to be paid to the time and temperature of sintering. Furthermore, frit fabrication depends on column I.D., particle size, and type of packing material (Colón et al, 2000). Because of all these drawbacks, different solutions have been suggested to retain the SP, thereby avoiding the sintering of silica.…”

“…Entrapment of particulate material by sintering and sol-gel technology (a hybrid between packed and monolithic columns) can also be mentioned as an alternative to frit formation (Colón et al, 2000).…”

Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography

“…The advance of CEC is reviewed by several authors [9][10][11][12]. The capillary columns with inner diameter of 50-100 mm packed with 1.5-10 mm conventional HPLC packing are typically used in CEC.…”