The image-processing method, based on Photoshop software, was used to analyze the fiber distribution pattern in yarn cross section for vortex-spun yarn. Fiber packing density and fiber effective packing density, as well as the fiber migration index, were investigated to provide a better understanding of the internal structures of vortex-spun yarn. The research results indicate that the vortex-spun yarn has a lower fiber packing density value than the conventional ring-spun yarn at the yarn center and surface. For the bamboo pulp fiber/white cotton blend vortex-spun yarn, the bamboo pulp fibers are more easily distributed in the yarn core. In addition, the coarser the vortex-spun yarn, the higher the migration level of the bamboo pulp fiber tending to migrate towards the inner layer of the blend yarn.