2002
DOI: 10.1590/s1516-93322002000100012
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Padronização de método analítico para avaliação do grau de exposição da fenilalanina em hidrolisados de caseína, por espectrofotometria derivada segunda

Abstract: Hidrolisados protéicos com baixo teor ou isentos de fenilalanina (Phe) têm sido empregados em formulações para fenilcetonúricos. Várias condições de hidrólise enzimática têm sido propostas na literatura para promover a liberação de Phe e, assim, facilitar sua remoção posterior. Neste trabalho, a espectrofotometria derivada segunda (EDS), um método analítico rápido, simples e de custo relativamente baixo, foi utilizada, inicialmente, para a caracterização dos aminoácidos aromáticos e, em seguida, para a determi… Show more

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Cited by 7 publications
(12 citation statements)
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“…2). The correlation coefficient and the curve equation were y ¼ 2:1748x þ 0:3913 and R 2 ¼ 0:9921: This result is in agreement with others in the literature (Ichikawa & Terada, 1977;Zhao, Sannier, Garreau, Lecoeur, & Piot, 1996) and also with a previous study carried out in our laboratory (Barbosa et al, 2002), since in all these works a linearity for the standard curve of Phe, in presence of Tyr and Trp in several concentrations, was shown.…”
Section: Linearity Of Standard Curve Of Phesupporting
confidence: 92%
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“…2). The correlation coefficient and the curve equation were y ¼ 2:1748x þ 0:3913 and R 2 ¼ 0:9921: This result is in agreement with others in the literature (Ichikawa & Terada, 1977;Zhao, Sannier, Garreau, Lecoeur, & Piot, 1996) and also with a previous study carried out in our laboratory (Barbosa et al, 2002), since in all these works a linearity for the standard curve of Phe, in presence of Tyr and Trp in several concentrations, was shown.…”
Section: Linearity Of Standard Curve Of Phesupporting
confidence: 92%
“…The similarity among the spectra of standard amino acids and proteins had previously been described by Ichikawa and Terada (1977), working with several native and denaturated proteins. The same result was previously achieved in our laboratory using papain for hydrolysing casein (Barbosa et al, 2002).…”
Section: Sds Spectrum Of Phesupporting
confidence: 85%
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“…A técnica de SE-HPLC, utilizada no presente trabalho, mostrou ser eficiente na caracterização de hidrolisados protéicos, especialmente quando o interesse está voltado para o fracionamento de peptídeos de baixas massas moleculares, ou seja, inferiores a 1000 Da (Silvestre et al, 1994a;Léonil et al, 2000;Morato et al 2000, Carreira et al, 2001Barbosa et al, 2002).…”
Section: Fracionamento Por Se-hplcunclassified