Paenalcaligenes niemegkensis sp. nov., a novel species of the family Alcaligenaceae isolated from plastic waste

Abstract

Strain NGK35T is a motile, Gram-stain-negative, rod-shaped (1.0–2.1 µm long and 0.6–0.8 µm wide), aerobic bacterium that was isolated from plastic-polluted landfill soil. The strain grew at temperatures between 6 and 37 °C (optimum, 28 °C), in 0–10 % NaCl (optimum, 1 %) and at pH 6.0–9.5 (optimum, pH 7.5–8.5). It was positive for cytochrome c oxidase, catalase as well as H2S production, and hydrolysed casein and urea. It used a variety of different carbon sources including citrate, lactate and pyruvate. The predominant membrane fatty acids were C16 : 1 cis9 and C16 : 0, followed by C17 : 0 cyclo and C18 : 1 cis11. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, followed by diphosphatidyglycerol. The only quinone was ubiquinone Q-8. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK35T belongs to the genus Paenalcaligenes (family Alcaligenaceae ), appearing most closely related to Paenalcaligenes hominis CCUG 53761AT (96.90 %) and Paenalcaligenes suwonensis ABC02-12T (96.94 %). The genomic DNA G+C content of strain NGK35T was 52.1 mol %. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus Paenalcaligenes . Based on phenotypic and molecular characterization, strain NGK35T can clearly be differentiated from its phylogenetic neighbours establishing a novel species, for which the name Paenalcaligenes niemegkensis sp. nov. is proposed. The type strain is NGK35T (=DSM 113270T=NCCB 100854T).

Draft Genome Sequence of Paenalcaligenes niemegkensis NGK35 T , Isolated from Plastic-Polluted Soil of an Abandoned Landfill

“… Paenalcaligenes niemegkensis NGK35 T was recently isolated in the frame of a study targeting potential polyethylene-degrading microorganisms and was described as a potential new member of the genus Paenalcaligenes , which includes three established species to date. The recently described Gram-negative bacterial strain ( 1 ) was isolated from plastic-polluted soil of an abandoned landfill in the eastern part of Germany (52°02′58.8″N, 12°39′34.8″E), which thus adds to the variety of environments in which this genus can be found. Other Paenalcaligenes members were isolated from human blood ( 2 ), from spent mushroom compost ( 3 ), or from the larval gut of the black soldier fly, Hermetia illucens ( 4 ).…”

Paenalcaligenes faecalis sp. nov., a novel species of the family Alcaligenaceae isolated from chicken faeces

“…The genus Paenalcaligenes belongs to family Alcaligenaceae and order Burkholderiales, which are found in multiple environments such as human blood, the larval intestine of Hermetia illucens, spent mushroom compost and plastic-polluted landfill soil [1][2][3][4]. Currently, there are four validly published species within the genus Paenalcaligenes, i.e.…”

Metatranscriptomics of microbial biofilm succession on HDPE foil: uncovering plastic-degrading potential in soil communities

“…Due to its high molecular weight and hydrophobicity, a successful microbial degradation requires the organisms to possess cellular properties which allow an interaction with the recalcitrant PE surface [19]. Even though a surprisingly large number of bacterial and fungal genera were found associated with PE degradation (Acinetobacter, Arthrobacter, Aspergillus, Bacillus, Brevibacillus, Klebsiella, Nocardia, Pseudomonas, Rhodococcus, Staphylococcus, Streptomyces) [19][20][21][22][23][24][25], so far only a few studies proposed biochemical mechanisms or enzymes expressed within PE-associated microorganisms [26][27][28]. Studies that screened microbial metagenomes for potential plastic-degrading enzymes from uncultivated microorganisms, have demonstrated the great potential of such Meta'omics techniques to unravel underlying mechanisms of PE degradation [29,30].…”