Paenibacillus ottowii sp. nov. isolated from a fermentation system processing bovine manure

Abstract

Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas. Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA–DNA relatedness through DNA–DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively. Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores. The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species. The G+C content of MS2379T is 45.9 mol%. Fermentation of glucose yields acid and gas end-products. The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine. The growth range of MS2379T was observed from 10–45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6–10 and up to 3 % NaCl. Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction. Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T. Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp. nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).

Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa

“… *Different result was reported in the original description by Montefusco et al [51]. †Data from Velazquez et al [38], Montefusco et al [51], von der Weid et al [52] and this study. ‡Calculated from genome. …”

“…Moderate amounts of one unidentified aminolipid (AL1), two unidentified glycolipids (GL1, GL2) and three lipids lacking a functional group (L1-L3) were determined. Strain UY79 T shared with its close relative P. ottowii [38] the same major polar lipids, except for GL3, AL1, GL1 and L1. On the other hand, strain UY79 T lacked phosphatidyl-monomethyl-ethanolamine and the unidentified aminoglycolipid that are present in P. ottowii.…”

“…Strain UY79 T shared with its close relative P. ottowii [38] the same major polar lipids, except for GL3, AL1, GL1 and L1. On the other hand, strain UY79 T lacked phosphatidyl-monomethyl-ethanolamine and the unidentified aminoglycolipid that are present in P.…”

Paenibacillus dendrobii sp. nov., an indole-3-acetic acid-producing endophytic bacterium isolated from Dendrobium nobile

“…To date, there are 242 validly published species of the genus Paenibacillus. They are widely distributed in distinct biotopes, such as rhizosphere [4,5], soil [6][7][8], insect gut [9,10], water springs [11,12] and fermentation systems [13,14]. Most members possess the ability to promote plant growth, produce antimicrobial compounds and yield enzymes utilized for bioremediation [15].…”