Paenibacillus pini sp. nov., a cellulolytic bacterium isolated from the rhizosphere of pine tree

Abstract

Strain S22(T), a novel cellulolytic bacterium was isolated from the rhizosphere of pine trees. This isolate was Gram-reaction positive, motile and rods, and formed terminal or subterminal ellipsoidal spores. S22(T) represented positive activity for catalase, oxidase, esterase (C4), esterase lipase (C8), beta-galactosidase, leucine arylamidase, and hydrolysis of esculin. It contained meso-diaminopimelic acid as the diagnostic dia-mino acid in the cell-wall. The predominant isoprenoid quinone was menaquinone 7 (MK-7), and the major cellular fatty acids were anteiso-C(15:0) (52.9%), iso-Ci(16:0) (11.3%), and iso-C(15:0) (10.0%). The DNA G+C content was 43.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this isolate belonged to the family Paenibacillaceae. S22(T) exhibited less than 97.0% 16S rRNA gene similarity with all relative type strains in the genus Paenibacillus, and the most closely related strains were Paenibacillus anaericanus MH21(T) and Paenibacillus ginsengisoli Gsoil 1638(T), with equal similarities of 95.8%. This polyphasic evidence suggested that strain S22(T) should be considered a novel species in the genus Paenibacillus, for which the name, Paenibacillus pini sp. nov., is proposed. The type strain is S22(T) (=KCTC 13694(T) =KACC 14198(T) =JCM 16418(T)).

A novel type bacterial flagellar motor that can use divalent cations as a coupling ion

“…Functional analysis of the cluster motility containing TCA-MotB1 has not yet been experimentally characterized, although each protein was automatically annotated as MotB in the database. Strains belonging to this cluster were isolated from various environments (e.g., soil, oral swab from patient, rhizosphere of plant, and assembly facility), and had no significant commonality 15 16 17 18 Paenibacillus lactis 154 belonging to this cluster was isolated from milk, which is rich in Ca 2+ 19 . Although P. lactis 154 was isolated from a Ca 2+ -abundant environment, the identity and similarity between TCA-MotB1 and MotB from P. lactis 154 were only 61% and 76%, respectively.…”

Paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (Lemna aequinoctialis)

“…The DNA G+C content is in the range of 39 to 54 mol% (Wang et al , 2012). Numerous species of the genus Paenibacillus have been isolated from diverse sources, including cold spring, soil, urban waste compost, clinical samples, the phyllosphere and the rhizosphere of various plant species (Tang et al , 2011; Wu et al , 2011; Vaz-Moreira et al , 2010; Glaeser et al , 2013; Valverde et al , 2008; Kim et al , 2009; Liu et al , 2010; Jin et al , 2011). In the present study, we carried out a pholyphasic investigation to characterize an endophytic bacterium, designated strain L7-75 T , which was isolated from duckweed ( Lemna aequinoctialis ).…”

Sphingomonas humi sp. nov., isolated from soil

“…The genus Sphingomonas belongs to the class Alphaproteobacteria (Takeuchi et al, 2001;Busse et al, 2003;Li et al, 2004;Rivas et al, 2004;Kim et al, 2007).In a series of studies, we attempted to isolate microorganisms from soil to investigate community structure based on a culture-dependent method (Duan et al, 2009;Kim et al, 2009) as well as the antimicrobial activity of plants against microorganisms growing in soil and food (Moon et al, 2009). In this study, an orange-colored Sphingomonas-like strain was isolated from soil near a pond located on the campus of the Korea Advanced Institute of Science and Technology (KAIST) in Daejeon city, South Korea.…”