Paenibacillus puldeungensis sp. nov., isolated from a grassy sandbank

Traiwan, Jitsopin; Park, Mi-Hak; Kim, Wonyong

doi:10.1099/ijs.0.022202-0

Cited by 22 publications

(6 citation statements)

References 22 publications

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“…bacterio.net/p/paenibacillus.html) and the type species is Paenibacillus polymyxa. Paenibacillus species are widely distributed throughout the biosphere such as blood (Ko et al 2008), garbage (Iida et al 2005), grassy sandbank (Traiwan et al 2011) and hot spring (Zhou et al 2012). Members of this genus have diverse physiological characteristics.…”

Section: Introductionmentioning

confidence: 99%

Paenibacillus vini sp. nov., isolated from alcohol fermentation pit mud in Sichuan Province, China

Chen

Shao

Wang

et al. 2015

Antonie van Leeuwenhoek

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A novel facultatively anaerobic bacterial strain, designated LAM0504(T), was isolated from a pit mud of Luzhou flavour liquor alcohol fermentation in Sichuan Province, China. Cells of strain LAM0504(T) were observed to be Gram-stain negative, spore-forming, rod shaped and motile by means of peritrichous flagella. Strain LAM0504(T) was found to be able to grow at 20-48 °C (optimum: 30 °C), pH 5.0-9.0 (optimum: 7.0) and 0-3 % NaCl (w/v) (optimum: 1.0 %). The 16S rRNA gene sequence similarity analysis showed that strain LAM0504(T) was most closely related to Paenibacillus konsisdensis JCM 14798(T), Fontibacillus phaseoli LMG 27589(T) and Paenibacillus motobuensis JCM 12774(T), with 97.0, 96.8 and 96.7 % sequence similarity, respectively. The DNA-DNA hybridization value between strain LAM0504(T) and P. konsisdensis JCM 14798(T) was 53.3 ± 1.2 %. The genomic DNA G+C content of strain LAM0504(T) was 43.0 mol% as determined by the Tm method. The major fatty acids of strain LAM0504(T) were identified as anteiso-C15:0, C16:0 and iso-C15:0. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified glycolipids and three unidentified lipids. On the basis of its physiological and phylogenetic characteristics, strain LAM0504(T) is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus vini sp. nov. is proposed. The type strain is LAM0504(T) (=ACCC 06420(T) = JCM 19842(T)).

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Section: Introductionmentioning

confidence: 99%

Paenibacillus vini sp. nov., isolated from alcohol fermentation pit mud in Sichuan Province, China

Chen

Shao

Wang

et al. 2015

Antonie van Leeuwenhoek

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“…The DNA G+C content is in the range of 39 to 54 mol% (Wang et al, 2012). Species of the genus Paenibacillus have been isolated from various environmental sources, such as soil, air, a sandbank, blood cultures, rhizosphere and plants (Ten et al, 2006;Rivas et al, 2005;Traiwan et al, 2011;Roux & Raoult, 2004;Kittiwongwattana & Thawai, 2015;Cao et al, 2015;Jiang et al, 2015;Xiang et al, 2014).…”

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confidence: 99%

Paenibacillus terreus sp. nov., isolated from forest soil

Huang

Dai

Zhou

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, rod-shaped, endospore-forming, motile bacterium, designated D33T , was isolated from a forest soil sample. The strain grew optimally at 30-37 8C, pH 8.0 and with 1 % (w/v) NaCl. The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus Paenibacillus. Strain D33 T contained meso-diaminopimelic acid in the cell-wall peptidoglycan, and ribose and lower amounts of glucose and galactose as the whole-cell sugars. The major cellular fatty acid was anteiso-C 15 : 0 , and menaquinone-7 (MK-7) was the only respiratory quinone. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, two glycolipids and an unknown lipid. The DNA G+C content was 51.1 mol%. The genus Paenibacillus, a member of the family Paenibacillaceae, was proposed by Ash et al. (1993Ash et al. ( , 1994. At the time of writing, there are 168 species with validly published names (http://www.bacterio.net/paenibacillus.html). Most members of the genus Paenibacillus are aerobic or facultatively anaerobic, rod-shaped, endospore-forming bacteria. The major cellular fatty acid is anteiso-C 15 : 0 and menaquinone-7 (MK-7) is the major menaquinone. The DNA G+C content is in the range of 39 to 54 mol% (Wang et al., 2012). Species of the genus Paenibacillus have been isolated from various environmental sources, such as soil, air, a sandbank, blood cultures, rhizosphere and plants (Ten et al., 2006;Rivas et al., 2005;Traiwan et al., 2011;Roux & Raoult, 2004;Kittiwongwattana & Thawai, 2015;Cao et al., 2015;Jiang et al., 2015;Xiang et al., 2014).In this study, the taxonomic characterization of strain D33 T using a polyphasic approach is reported and in conclusion, a novel species of genus Paenibacillus is proposed. Strain D33T was isolated from a forest soil sample and purified on a sucrose-mineral salts medium as described previously (Huang et al., 2014(Huang et al., , 2015. The isolate was maintained on trypticase soy agar [TSA, comprising trypticase soy broth (TSB) supplemented with 15 g Bacto Agar (Difco)]. The morphology of cells, spores and motility was examined by light microscopy (CX21; Olympus). The presence of flagellum was examined by transmission electron microscopy (TEM, H-7650; Hitachi) using cells from the exponential phase. The morphology and size of colonies were observed on sucrose-mineral salts medium and TSA plates after 2 days at 30 8C. Growth at different temperatures (4, 10, 20, 30, 37, 42, 45, 50 and 55 8C) was determined on TSA in an aerobic incubator. TSB was used in experiments to determine growth at pH 4-10 (at intervals of 1 pH unit; the pH was adjusted with HCl or Na 2 CO 3 ) and the requirement for NaCl (0-5 %, w/v, at intervals of 1.0 %). Catalase activity was determined according to bubble production in a 3 % (v/v) hydrogen peroxide solution. Oxidase activity was determined based Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PME, phosph...

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“…The genus Paenibacillus comprises aerobic, Gram-staining-positive, motile, rod-shaped bacteria that are characterized chemotaxonomically by the presence of MK-7 as the major isoprenoid quinone, meso -diaminopimelic acid as the cell-wall peptidoglycan type, and anteiso-C 15 : 0 as the predominant cellular fatty acid (Shida et al 1997a, b). Recently several species of the genus Paenibacillus have been isolated from various habitats including glacier ( Paenibacillus glacialis ; Kishore et al , 2010), wetland ( Paenibacillus rigui ; Baik et al , 2011), sandbank ( Paenibacillus puldeungensis ; Traiwan et al , 2011), a molybdenum mine ( Paenibacillus phoenicis ; Benardini et al , 2011), a cold spring ( Paenibacillus algorifonticola ; Tang et al , 2011), tidal flat ( Paenibacillus sediminis ; Wang et al , 2012), sediment of a hot spring ( Paenibacillus thermophilus ; Zhou et al , 2012), naked barley ( Paenibacillus hordei ; Kim et al , 2013), marine sediment ( Paenibacillus oceanisediminis ; Lee et al , 2013), a necrotic wound ( Paenibacillus vulneris ; Glaeser et al , 2013), roots ( Paenibacillus typhae ; Kong et al , 2013) and rhizosphere soil ( Paenibacillus catalpae ; Zhang et al , 2013).…”

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confidence: 99%