Paenibacillus tibetensis sp. nov., a psychrophilic bacterium isolated from alpine swamp meadow soil

Abstract

A novel psychrophilic strain, SSB001 T , was isolated from an alpine swamp meadow soil in Tibet, China, and identified as a representative of a novel phylogenetic subclade in the genus Paenibacillus, with Paenibacillus antarcticus (96.2 %), Paenibacillus macquariensis (96.53 %) and Paenibacillus glacialis (96.2 %) as the most closely related species on the basis of 16S rRNA gene sequence analyses. The strain was distinguished from defined species of the genus Paenibacillus by further study of rpoB gene sequences, phenotypic characterization, cellular fatty acid composition, quinones, polar lipids and meso-diaminopimelic acid in the peptidoglycan. Based upon these results, we propose the strain as a representative of a novel species named Paenibacillus tibetensis sp. nov., with SSB001 T (5ACCC 19728 T 5DSM 29321 T) as the type strain. The DNA G+C content (mol%) of strain SSB001 T was 40.18 mol% (HPLC). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and rpoB gene sequences of strain SSB001 T are KM522843 and KM522842, respectively. Four supplementary figures are available with the online Supplementary Material.