Paeonia lactiflora Enhances the Adhesion of Trophoblast to the Endometrium via Induction of Leukemia Inhibitory Factor Expression

Choi, Hong‐Seok; Chung, Tae‐Wook; Park, Mi-Ju; Lee, Kyu Sup; Yoon, Young-Jin; Kim, Hyung Sik; Lee, Jun Hee; Kwon, Sang‐Mo; Lee, Syng‐Ook; Kim, Keuk‐Jun; Baek, Jangsun; Ha, Ki‐Tae

doi:10.1371/journal.pone.0148232

Cited by 20 publications

(26 citation statements)

References 49 publications

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“…As ST6GalNAc1 is responsible for the expression of sialyl-Tn antigen and termination of O-glycan ( Fig. 2A), we confirmed the expression of the ST6GalNAc1 gene in the receptive endometrial Ishikawa and non-receptive AN3CA cells [21,27]. The results shown in Fig.…”

Section: St6galnac1 Expression Mediated Adhesion Of Trophoblast To Ensupporting

confidence: 75%

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Choi¹,

Chung²,

Kim³

et al. 2020

Preprint

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BackgroundTo characterize molecular mechanism underlying the regulation of sialylated glycan expression and its roles for endometrial receptivity and embryo implantation. Here, we characterized the role of a truncated form of sialylated O-glycan, sialyl-Tn, for endometrial receptivity. MethodsThe transcriptomes of human endometrium at mid-secretory phase were analyzed by Bioinformatics.Changes in gene expression, protein, and signal pathway were measured using RT-PCR and Western blot. The cell adhesion assay was visualized using a fluorescent microscope. In peri-implantation phase of mice, the expression of leukemia inhibitory factor (LIF) and sialyl-Tn were confirmed using immunohistochemistry and immnofluorescence analysis. The effect of sialy-Tn expression on embryo implantation was estimated by in vitro fertilization and embryo transfer using mice. ResultsIn in silico analysis, expression of O-glycosylation genes, especially ST6GalNAc1, was significantly increased in the human uterus of mid-secretory phase. Overexpression of the ST6GalNAc1 gene in non-receptive human endometrial AN3CA cells enhances the attachment of trophoblastic JAr cells. In an animal study, the results clearly indicated that sialyl-Tn was expressed on the surface of the midsecretory uterus. In addition, blockade of the receptor using free sialyl-Tn epitope diminished the implantation rates of intrauterine transferred murine embryos. ConclusionFrom these results, here we suggest that sialyl-Tn expression might be a novel factor regulating the endometrial receptivity for successful embryo implantation. BackgroundEmbryo implantation, a key process for a successful pregnancy, is one of the remaining unmet needs to improve the success rate of assisted reproduction [1]. The endometrium is receptive to embryo only during the implantation window, a short period of time about 6-10 days after ovulation [1,2]. A large number of molecular mediators including adhesive proteins, hormones, cytokines, and growth Authors' contributions HJC and TWC designed the experiments and performed the major molecular experiment. JK and JHJ performed in vitro fertilization and embryo transfer. BSK and MJP assisted the molecular experiments. KJK perfoermed the immunohistochemistry and immunoflurescent staining. JKJ and CHK analyzed the

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Section: St6galnac1 Expression Mediated Adhesion Of Trophoblast To Ensupporting

confidence: 75%

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Choi¹,

Chung²,

Kim³

et al. 2020

Preprint

Self Cite

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“…In all 11 studies, in vivo studies were performed rather than in vitro. All 11 studies included in vivo studies; Choi et al [ 9 ] conducted both in vivo and in vitro studies.…”

Section: Resultsmentioning

confidence: 99%

“…The models were either COH induction or EID induction. Embryo implantation dysfunction (EID) model was used in six studies [ 6 , 9 – 13 ] and controlled ovarian hyperstimulation (COH) model in four studies [ 14 – 17 ]. In one study, both EID model and COH model were examined [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Modernization Trends of Infertility Treatment of Traditional Korean Medicine

Park

Kim

2017

Evidence-Based Complementary and Alternative Medicine

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Despite the development of assisted reproductive technology (ART), it is difficult to increase the implantation rate. In Korea, Traditional Korean Medicine, including herbal medicine, is an important component of infertility treatment. Korean medical doctors who are treating infertility often use herbal medicine to promote implantation. In this article, as one of the research works on modernization of Traditional Korean Medicine, we investigated the experimental studies to clarify the effects of herbal medicines that are traditionally used to promote pregnancy. We searched for experimental studies over the past 10 years of improvement of endometrial receptivity in herbal medicine using six domestic and international sites. We analyzed 11 studies that meet the selection criteria. We found that herbal medicines demonstrably improved endometrial receptivity and increased pregnancy rates.

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“…To resolve this problem, assisted reproductive technologies (ART) have been steadily developed [8,9]. Many previous studies have sought substances such as calcitonin [10], low-dose aspirin [11], GnRH analogues [12] and natural substances extracted from Paeonia lactiflora [13], Perilla frutescens [14], Cyperus rotundus L. [15], Scutellaria baicalensis [16] that can help to increase endometrial receptivity. Since traditional herbal remedies have been proven to be safe when administered orally, many researchers have focused on natural substances [13][14][15][16][17]; however, failure of implantation still remains the main challenge in the success of ART [8,9].…”

Section: Introductionmentioning

confidence: 99%

Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation

Kim

Lee

Han

et al. 2020

BMC Complement Med Ther

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Background: Embryo implantation is essential for a successful pregnancy, and an elaborate synchronization between the receptive endometrium and trophoblast is required to achieve this implantation. To increase 'endometrial receptivity', the endometrium undergoes transformation processes including responses of adhesion molecules and cellular and molecular cell to cell communication. Many natural substances from traditional herbs have been studied to aid in the achievement of successful implantation. In this study, we investigated positive effects on embryonic implantation with decursinol that is a major compound extracted from Angelica gigas Nakai known to be associated with promotion of healthy pregnancy in the traditional Korean herbal medicine. Methods: Expression of cell adhesion molecules after treatment of endometrial epithelial cells by decursinol (40 or 80 μM) was determined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot analysis. The alteration of endometrial receptivity by decursinol (40 or 80 μM) was identified with the in vitro implantation model between Ishikawa cells and JAr cell spheroids (diameter, 143 ± 16 μm). Exosomes secreted from Ishikawa cells after treatment of 80 μM decursinol or dimethyl sulfoxide (DMSO) as the vehicle were investigated with invasion of JAr cells and attachment of JAr spheroids to Ishikawa cells. Results: Decursinol significantly (P < 0.05) increased the expression of important endometrial adhesion molecules such as integrin β1, β3, β5 and L-selectin mRNAs and integrin β5 and L-selectin in protein. The adhesion rate of JAr spheroids to decursinol-treated Ishikawa cells also increased significantly which was 2.4-fold higher than that of the control (P < 0.05). Furthermore, decursinol induced an increase in the release of exosomes from Ishikawa cells and decursinol-induced exosomes showed autocrine (to Ishikawa cells) and paracrine (to JAr cells) positive effects on our implantation model. Conclusion: These results propose that decursinol could serve as a new and alternative solution for patients who are infertile.

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Paeonia lactiflora Enhances the Adhesion of Trophoblast to the Endometrium via Induction of Leukemia Inhibitory Factor Expression

Cited by 20 publications

References 49 publications

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Modernization Trends of Infertility Treatment of Traditional Korean Medicine

Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation

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