PAFAH1B1 and the lncRNA <i>NONHSAT073641</i> maintain an angiogenic phenotype in human endothelial cells

Josipovic, Ivana; Fork, Christian; Preussner, Jens; Prior, Kim-Kristin; Iloska, Dijana; Vasconez, Andrea E.; Labocha, Sandra; Angioni, Carlo; Thomas, Dominique; Ferreiròs, Nerea; Looso, Mario; Pullamsetti, Soni Savai; Geißlinger, Gerd; Steinhilber, Dieter; Brandes, Ralf P.; Leisegang, Matthias

doi:10.1111/apha.12700

Cited by 27 publications

(12 citation statements)

References 51 publications

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“…Quantification of 20-HETE was done in principle as described previously 66 . In brief, 200 μl supernatant were spiked with the corresponding deuterated internal standards and extracted by liquid–liquid extraction using ethyl acetate.…”

Section: Methodsmentioning

confidence: 99%

20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1

et al. 2018

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The long-chain fatty acid receptor FFAR1 is highly expressed in pancreatic β-cells. Synthetic FFAR1 agonists can be used as antidiabetic drugs to promote glucose-stimulated insulin secretion (GSIS). However, the physiological role of FFAR1 in β-cells remains poorly understood. Here we show that 20-HETE activates FFAR1 and promotes GSIS via FFAR1 with higher potency and efficacy than dietary fatty acids such as palmitic, linoleic, and α-linolenic acid. Murine and human β-cells produce 20-HETE, and the ω-hydroxylase-mediated formation and release of 20-HETE is strongly stimulated by glucose. Pharmacological inhibition of 20-HETE formation and blockade of FFAR1 in islets inhibits GSIS. In islets from type-2 diabetic humans and mice, glucose-stimulated 20-HETE formation and 20-HETE-dependent stimulation of GSIS are strongly reduced. We show that 20-HETE is an FFAR1 agonist, which functions as an autocrine positive feed-forward regulator of GSIS, and that a reduced glucose-induced 20-HETE formation contributes to inefficient GSIS in type-2 diabetes.

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Section: Methodsmentioning

confidence: 99%

20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1

et al. 2018

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“…LncRNA AK094457 was shown to enhance iNOS and nitric oxide (NO) expression in vascular ECs, suggesting a potential role in regulating blood pressure 132 . In an independent study, LncRNA NONHSAT073641 is upregulated in lung tissues from subjects with chronic thromboembolic pulmonary hypertension (CTEPH) and pulmonary veno-occlusive disease (PVOD) 133 . This lncRNA is highly homologous to the protein-coding gene, platelet-activating factor acetyl hybrdolase (PAFAH1B1).…”

Section: Lncrnas In Vascular Diseasementioning

confidence: 99%

Angio-LncRs: LncRNAs that regulate angiogenesis and vascular disease

Yu¹,

Wang²

2018

Theranostics

167

130

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Long noncoding RNAs (lncRNAs) represent a large subgroup of RNAs that are longer than 200 nucleotides and have no apparent protein coding potential. They have diverse functions in different biological processes by regulating chromatin remodeling or protein translation. This review summarizes the recent progress of lncRNAs in angiogenesis and vascular diseases. A general overview of lncRNA functional mechanisms will be introduced. A list of lncRNAs, which are termed “Angio-LncRs”, including MALAT1, MANTIS, PUNISHER, MEG3, MIAT, SENCR and GATA6-AS, will be discussed regarding their expression, regulation, function and mechanism of action in angiogenesis. Implications of lncRNAs in vascular diseases, such as atherosclerosis, hypertension, vascular retinopathies and tumor angiogenesis will also be discussed.

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“…Our previous studies have found there are 1614 differential mRNA genes (Fold change > 1.2 and P-value < 0.05), 185 lncRNAs (Fold change > 1.2 and P-value < 0.05) and 25 miRNAs (Fold change > 2 and P-value < 0.05) between the experimental and the control group. [11][12][13] Target genes predicted by miRNA and intersective genes 1: The number of target genes of 25 miRNAs predicted by miRanda is 10301. We intersected these genes with the 1614 differential mRNA genes from the microar-WANG, ET AL Figure 1.…”

Section: Microarray Analysis Outcomesmentioning

confidence: 99%

“…Another study demonstrated that lncRNA ENSG 00000262454 correlated with pericyte markers in idiopathic pulmonary arterial hypertension. 10) In the case of CTEPH, Josipovic, et al 11) found that lncRNA NONHSAT 073641 was upregulated in the vascular samples of pa-miRNA-genes-lncRNA NETWORK IN CTEPH tients with CTEPH and were important for endothelial angiogenic function. Competing endogenous RNAs (ceRNA) was found to lead to the hypothesis that lncRNA could interact with the gene through microRNA (miRNA) by competing for endogenous inhibition.…”

mentioning

confidence: 99%

miRNA-PDGFRB/HIF1A-lncRNA CTEPHA1 Network Plays Important Roles in the Mechanism of Chronic Thromboembolic Pulmonary Hypertension

Wang

Liu

et al. 2019

Int. Heart J.

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Our previous studies have revealed that long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and genes were abnormally expressed in the pulmonary artery tissues of the chronic thromboembolic pulmonary hypertension (CTEPH) patients. We aim to establish the CTEPH-related miRNA-gene-lncRNA network for finding the core genes and associated miRNA and lncRNA in CTEPH patients.Firstly, the target genes of differential miRNAs were predicted by searching TargetScan databases, and the predicted target genes were intersected with the mRNAs from the gene chip. Secondly, the intersective genes were analyzed by the Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway software for obtaining differential intersective genes and then establish the miRNA-gene networks. Thirdly, the possible genes regulated by the differential lncRNAs from the gene chip were intersected with the above-screened mRNA to build the lncRNA-mRNA networks. Subsequently, the miRNA-gene-lncRNA networks were constructed according to the two networks above(miRNA-gene networks and lncRNA-mRNA networks). Finally, the core genes of the networks in the experimental group were screened according to Diffk > 0.6 and used to construct the miRNA-core gene-lncRNA networks of CTEPH.The pathway network, miRNA-mRNA network, lncRNA-mRNA networks, and miRNA-gene-lncRNA networks were successfully constructed. The core genes of the miRNA-gene-lncRNA networks (Diffk > 0.6) were the human Beta-type platelet-derived growth factor receptor (PDGFRB) and hypoxia-inducible factor-1a (HIF-1 A), the miRNAs-PDGFRB-lncRNAs and miRNAs-HIF1A-lncRNAs networks were constructed. Finally, miRNA-149-5p-PDGFRB-TCONS_l2_00020587-XLOC_l2_010723 and miRNA-338-5p/miRNA-199b-5p-HIF1 A-TCONS_l2_00020587-XLOC_l2_010723 were found in the analysis of the network.miRNA-149-5p-PDGFRB-lncRNA CTEPH-associated 1 (CTEPHA1) (TCONS_l2_00020587-XLOC_l2_ 010723) and miRNA-338-5p/miRNA-199b-5p-HIF1A-lncRNA CTEPHA1 are related to the development of CTEPH.(Int Heart J 2019; 60: 924-937)

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PAFAH1B1 and the lncRNA NONHSAT073641 maintain an angiogenic phenotype in human endothelial cells

Abstract: PAFAH1B1 and NONHSAT073641 are important for endothelial angiogenic function.

Cited by 27 publications

References 51 publications

20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1

20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1

Angio-LncRs: LncRNAs that regulate angiogenesis and vascular disease

miRNA-PDGFRB/HIF1A-lncRNA CTEPHA1 Network Plays Important Roles in the Mechanism of Chronic Thromboembolic Pulmonary Hypertension

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