Paired comparison of apheresis platelet function after storage in two containers

Abstract

Platelet quality after storage strongly depends on the pre-storage quality as well as on the storage conditions determined by the storage container. In this paired study, we evaluated two different containers . The Fresenius AS104 cell separator was used to prepare 17 platelet concentrates that were split and distributed into the containers to be compared. Cell counts, blood gas analysis, morphological scores, glucose and lactate levels, platelet activation, and platelet aggregation were measured before splitting at the day of preparation and after storage at day 3 and day 5. At day 3, there was no significant difference between the two bags apart from increased lactate and decreased pCO 2 concentrations in the CLX™ bags. At day 5 there were significantly higher lactate concentrations, pO 2 levels, and aggregation after stimulation in the CLX™ group, while the glucose and pCO 2 concentrations were significantly lower in these platelet concentrates as compared to the DPL-110 group. However, these parameters did not influence the functional parameters tested. While the platelet quality decreased during storage in all bags, the functional changes were nearly identical in both bags tested. We conclude that both bags are equivalent for 5-day storage of platelet concentrates.

A rest period before agitation may improve some in vitro apheresis platelet parameters during storage

“…The CLX and PL2410 containers had adequate oxygen permeabilities (1300 mL/m 2 × day × atm, for CLX, and 2024 mL/m 2 × day × atm, for PL2410). A ratio of PLTs to plasma volume was 1332 × 10 9 and 1300 × 10 9 /L in PL2410 and CLX containers, respectively and was comparable to previously reported values 9‐11 . One bag was placed on a reciprocal agitator (65‐70 cycles/min, Helmer platelet storage system, Helmer Labs, Inc., Noblesville, IN) immediately after transfer to the CLX container and stored with constant agitation at 20 to 24°C.…”

First comparison of productivity and citrate donor load between the Trima® version 4 (dual‐stage filler) and the Trima Accel® (single‐stage filler) in the same donors

“…Regarding the latter, one may conclude that this system has the ability to produce PLT apheresis concentrates in a safe and, especially, economic manner. From our experience, citrate reactions very rarely occur at ACDA flow rates of up to 1·1 ml ACDA/min/l of donor blood volume [9,16,20,39]. Thus, it may be possible to increase individually the citrate flow rates for donors who are not sensitive to citrate, in order to reduce, even further, the procedure time without increasing the incidence of side‐effects such as citrate reactions.…”

Short‐term liquid storage of CD14+ monocytes, CD11c+, and CD123+ precursor dendritic cells produced by leukocytapheresis

“…The COBE Spectra apheresis machine (Software Version 5.1) equipped with the WBC dual‐needle set disposes of a 500‐mL POF storage container (bag surface excluding the label, 622 cm 2 ) with the following gas transfer rates: O 2 , 2.5 L per m 2 per 24 hours per bar; and CO 2 , 16.6 L per m 2 per 24 hours per bar 14 . Five MNC procedures were performed either with the standard WBC program (separation factor, SF500) or with a reduced separation factor (SF250).…”