Short‐term liquid storage of CD14+ monocytes, CD11c+, and CD123+ precursor dendritic cells produced by leukocytapheresis

Abstract

Storage of MNCs within 72 hours in the original harvest container assures stable WBC content and is easy to perform. POF bags should be preferred in the case of extended WBC storage. Patient studies should clarify changes in efficiency of hematopoietic reconstitution that might occur over time during MNC storage.

Peripheral blood mononuclear cells obtained from leukoreduction system chambers show better viability than those from leukapheresis

“…According to observations of Picker and coworkers, 2 oxygen consumption rate inversely correlated with cell damage analyzed by JC‐1 in a study about PLT storage. A decrease of pH is known to correlate very well with lactate production in case of PBMNC storage 3 . In our study pH inversely correlated with annexin V+/7‐AAD+ PBMNCs in leukapheresis products (lymphocytes, R 2 = 0.741, p < 0.001; monocytes, R 2 = 0.601, p < 0.001).…”

Bags versus flasks: a comparison of cell culture systems for the production of dendritic cell–based immunotherapies

“…The most commonly known examples of polyolefins include polyethylene and polypropylene. Polyolefin‐based cell containers have been tested for both in vitro culture and storage of CD34+ cells, platelets, DCs, and T cells . In the case of monocyte‐derived APCs, different monocyte isolation methods may alter the composition of “contaminating” cell populations and proteins entrained with the monocytes seeded, which may in turn impact the propensity of monocytes to adhere to surfaces and to differentiate into APCs.…”

“…Polyolefin-based cell containers have been tested for both in vitro culture and storage of CD341 cells, platelets, DCs, and T cells. 60,66,67,72,[84][85][86] In the case of monocyte-derived APCs, different monocyte isolation methods may alter the composition of "contaminating" cell populations and proteins entrained with the monocytes seeded, which may in turn impact the propensity of monocytes to adhere to surfaces and to differentiate into APCs. * The typical working volume is 0.2-0.5 mL/cm 2 for flasks or layered vessels, 4-11 mL for the membrane-bottom vessel system, or 0.5-1 mL/cm 2 for bags.…”

Low‐oxygen and high‐carbon‐dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia

“…The data on improvement of fresh blood and separated blood cell storage at 4°C are complex and controversial. They include evaluation of different temperature and storage solution composition, 3,5,6 the effect of air in storage vials, and gas permeability of collection bags 7‐9 …”

“…They include evaluation of different temperature and storage solution composition, 3,5,6 the effect of air in storage vials, and gas permeability of collection bags. [7][8][9] Storage at 4°C, for example, in hypothermia results in a specific metabolic state termed "hypometabolism. "…”