Peripheral blood mononuclear cells obtained from leukoreduction system chambers show better viability than those from leukapheresis

Quantity, quality, and functionality of peripheral blood cells derived from residual blood of different apheresis kits

“…In conclusion, we confirmed the applicability of LRSC as an economic and valid source for PBMC isolation and further subpopulation analysis . Previously published evaluations on cell yield and cellular subpopulations were now extended to provide important information on functionality in terms of cytokine production capability and killing activity.…”

“…Research with human material such as PBMCs and immune cells strongly depends on the availability of PBMC sources, which can be a limiting factor, especially if large quantities are required. Whole blood, WBC depletion filters, buffy coats, and LRSCs from the Trima Accel plateletpheresis device are commonly used starting material for PBMC isolation and further usage of different subpopulations . The PBMC source for research purposes is mostly determined by the local blood donation service based on the blood collection systems established in their daily routine.…”

“…Whole blood, WBC depletion filters, buffy coats, and LRSCs from the Trima Accel plateletpheresis device are commonly used starting material for PBMC isolation and further usage of different subpopulations. 2,[8][9][10][12][13][14][15]24,25 The PBMC source for research purposes is mostly determined by the local blood donation service based on the blood collection systems established in their daily routine. In this study, we evaluated quantity and quality of PBMCs isolated from apheresis kits of the AMICUS and Trima Accel preparative hemapheresis devices, the latter having been our standard source for many years.…”

Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T‐cell subpopulations

“…Unfortunately, compared with PBSCs, there is a lack of understanding about how T‐cell populations tolerate the freeze and thaw process. Several studies performed on a variety of cryopreserved related donor leukocyte products found that CD3+ postthaw cell recoveries ranged from 50% to 90% . However, the heterogeneous nature and/or processing of the clinical products did not allow a fair comparison between different methods of cryopreservation.…”

Leukocyte reduction filters as an alternative source of peripheral blood leukocytes for research

“…The gold standard to obtain these cells is leukapheresis. 18 , 19 However, the use of buffy coats or leukapheresis products for research purposes is highly expensive and is not available at every blood bank. Several groups have examined the phenotype and function of immune cells isolated from LRFs, compared to standard buffy coats.…”