Palatal expansion and relapse in rats: A histologic and immunohistochemical study

Yang, Cheng; Lv, Chunxiao; Li, Tiancheng; Zhang, Cheng; Li, Rui; Tao, Guiyu; Su, Chenxi; Huang, Li; Zou, Shujuan; Chen, Jianwei

doi:10.1016/j.ajodo.2019.06.017

Cited by 10 publications

(9 citation statements)

References 24 publications

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Section: Discussionmentioning

confidence: 87%

“…To elucidate these questions, we established a murine model in which each rat underwent 7 days of expansion and 7 days of relapse. Reliability and reproducibility of this rat model were corroborated using Micro-CT and histological and immunohistochemical analyses (Cheng et al, 2020). In this study, the rat (2) a 7-day expansion is more time-effective compared with a 14-day expansion, with bone formation reaching its highest level on D7 (Wu et al, 2017); (3) the force magnitude was altered from 50 g to 100 g, because in a study (Zahrowski & Turley, 1992) investigating the effect of force magnitude on maxillary sutures, the number of active mitotic cells peaked at 100 g force, and the reliability of 100 g force magnitude on palate expansion was also substantiated in a previous study (Cheng et al, 2018).…”

Section: Discussionmentioning

confidence: 92%

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Gavage‐administered lactoferrin promotes palatal expansion stability in a dose‐dependent manner

et al. 2021

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Objective To investigate the effects of different lactoferrin concentrations on mid‐palatal suture bone remodeling during palatal expansion and relapse in rats. Materials and Methods Thirty‐two 5‐week‐old male Wistar rats were randomly divided into four groups: EO (expansion only), E+LF1 (expansion plus 10 mg/kg/day daily LF), E+LF2 (expansion plus 100 mg/kg/day daily LF), and E+LF3 (expansion plus 1 g/kg/day daily LF). Thereafter, micro‐computed tomography and micro‐morphology of the mid‐palatal suture were analyzed on day 7 and day 14, respectively. Results The arch widths were increased in all the four groups after expansion, and there was no significant difference among them on day 7. After relapse, however, the arch width in the E+LF3 group was significantly larger compared with EO group. In E+LF3 group and E+LF2 group, new bone formation and osteoblast number were enhanced with up‐regulated expression of osteocalcin and collagen type I, while the expression of cathepsin K‐positive cells was downregulated in E+LF3 group. Conclusion Lactoferrin gavage administration might increase the stability of palatal expansion and reduce relapse in a concentration‐dependent manner by enhancing bone formation and inhibiting resorption. LF administration may be promising for optimizing the maxillary expansion outcome.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 92%

Gavage‐administered lactoferrin promotes palatal expansion stability in a dose‐dependent manner

et al. 2021

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“…These observations correspond with the histological changes reported in other studies. 22 However, STAT3 inhibition disrupted these normal histological changes. Periosteal progenitors are committed to the osteogenic lineage with upregulated STAT3 phosphorylation.…”

Section: Discussionmentioning

confidence: 98%

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

et al. 2023

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Objectives The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone formation during palatal expansion and relapse. Methodology In total, 30 male Wistar rats were randomly allocated into Ctrl (control), E (expansion only), and E+Stattic (expansion plus STAT3-inhibitor, Stattic) groups. Micro-computed tomography, micromorphology staining, and immunohistochemistry of the mid-palatal suture were performed on days 7 and 14. In vitro cyclic tensile stress (10% magnitude, 0.5 Hz frequency, and 24 h duration) was applied to rat primary osteoblasts and Stattic was administered for STAT3 inhibition. The role of STAT3 in mechanical loading-induced osteoblasts was confirmed by alkaline phosphatase (ALP), alizarin red staining, and western blots. Results The E group showed greater arch width than the E+Stattic group after expansion. The differences between the two groups remained significant after relapse. We found active bone formation in the E group with increased expression of ALP, COL-I, and Runx2, although the expression of osteogenesis-related factors was downregulated in the E+stattic group. After STAT3 inhibition, expansive force-induced bone resorption was attenuated, as TRAP staining demonstrated. Furthermore, the administration of Stattic in vitro partially suppressed tensile stress-enhanced osteogenic markers in osteoblasts. Conclusions STAT3 inactivation reduced osteoblast-mediated bone formation during palatal expansion and post-expansion relapse, thus it may be a potential therapeutic target to treat force-induced bone formation.

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“…Maxillary discrepancy is one of the most common skeletal problems in orthopedic practice, which can mainly cause crossbite and dental crowding [13]. An adequate procedure for correction of skeletal imbalances is to manipulate and control growth of the maxilla.…”

Section: ⅳ Discussionmentioning

confidence: 99%

Assessment of Midpalatal Suture Maturation by Skeletal Maturity on Hand Wrist Radiographs

Yu¹,

Kim²

2021

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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The aim of this retrospective study was to evaluate the correlation between the midpalatal suture maturation and skeletal maturation in growing children aged 7 - 15 years and predict the maturational stages of the midpalatal suture corresponding to skeletal maturity assessed by the skeletal maturation indicators (SMI) and middle phalanx of the third finger (MP3) stages.The group of this retrospective study was consisted of randomly selected 132 male and 135 female in age from 7 - 15 years. The maturation of the midpalatal suture was evaluated by using images from cone-beam computed tomography (CBCT) while the skeletal age was assessed by hand-wrist radiography. CBCT images and hand-wrist radiographs used in this study were obtained from all subjects for orthodontic diagnosis before orthodontic treatment.The maturational stages of the midpalatal suture showed strong correlations with both SMI and MP3 stages. The correlation between the midpalatal suture maturation and SMI (Spearman’s correlation coefficient, ϒS = 0.905, p < 0.05) was slightly greater than that of MP3 stages (ϒS = 0.830, p < 0.05). There was a positive significant correlation between the midpalatal suture maturation and chronological age (ϒS = 0.868,p < 0.05).CBCT for evaluation of the midpalatal suture maturational stages may be unnecessary in every pediatric patients because SMI and MP3 stages were both replaceable useful methods for assessing maturation of the midpalatal suture before orthopedic treatment. In this retrospective study, the diagnostic reliability of the SMI method for estimating midpalatal suture maturation showed better reliability than the MP3 method.

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Palatal expansion and relapse in rats: A histologic and immunohistochemical study

Cited by 10 publications

References 24 publications

Gavage‐administered lactoferrin promotes palatal expansion stability in a dose‐dependent manner

Gavage‐administered lactoferrin promotes palatal expansion stability in a dose‐dependent manner

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

Assessment of Midpalatal Suture Maturation by Skeletal Maturity on Hand Wrist Radiographs

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