With the research on the issue in its initial phases, the behaviour and hunting strategies of MSA communities inhabiting the Nile Valley in the Late and Terminal Pleistocene have been fragmentarily recognised thus far. Osteological materials from the area of the Affad Basin in the Middle Nile Valley, recorded in archaeological contexts and dated to the sixteenth millennium BP using OSL methods, have significantly enhanced our knowledge in this regard. It is the first time that an opportunity has occurred to construct a reliable model of the environment exploitation and the behaviour of human groups producing lithic tools using Levallois methods in the Terminal Pleistocene. Archaeozoological analyses have allowed the identification of taxa, species and anatomical origin of remains and enabled the establishment of a database of osteometric measurements. The animals hunted in the Sudanese Nile Valley during the Terminal Pleistocene have been classified with a view to refer the data to the results of analogous studies on MSA in South Africa. The behaviour of the communities occupying the Affad Basin 15,000 years ago was connected to the environment of the tree-covered, swampy savannah and extensive backwaters. Medium-sized antelope (kobus) was hunted most often. People hunted also, albeit less frequently, for large ruminants (buffalo), guenons and large rodents. Remains of fish and mega-fauna (hippopotamus and elephant) have been found in isolated concentrations, away from the camp sites. Remains of molluscs or ostrich eggs have not been registered. The condition of the osteological materials, notably their anatomical distribution, is shown to have been largely affected by wetland environment, rich in iron and manganese.