PAMAM G4 dendrimers lower high glucose but do not improve reduced survival in diabetic rats

Łabieniec, Magdalena; Uličná, O; Vančová, Oľga; Głowacki, Rafał; Šebeková, Katarína; Bald, Edward; Gabryelak, Teresa; Watała, Cezary

doi:10.1016/j.ijpharm.2008.08.001

Cited by 33 publications

(37 citation statements)

References 39 publications

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“…9) (Labieniec & Watala, 2010). It was also evidenced that PAMAM dendrimer G4 administrated to rats with streptozotocin-diabetes acted as glucose scavenger and suppressed the accumulation of AGEs products, as well as some other markers of oxidative and carbonyl stress (Labieniec et al, 2008).…”

Section: New Agents -New Hopes and New Perspectivesmentioning

confidence: 93%

Mitochondria Function in Diabetes – From Health to Pathology – New Perspectives for Treatment of Diabetes-Driven Disorders

Łabieniec-Watała¹,

Siewiera²,

Gierszewski³

et al. 2012

Biomedical Science, Engineering and Technology

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Section: New Agents -New Hopes and New Perspectivesmentioning

confidence: 93%

Mitochondria Function in Diabetes – From Health to Pathology – New Perspectives for Treatment of Diabetes-Driven Disorders

Łabieniec-Watała¹,

Siewiera²,

Gierszewski³

et al. 2012

Biomedical Science, Engineering and Technology

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“…IC 50 values (defined as the concentration for which 50% release of haemoglobin was observed) for PAMAM G2, G3 and G4 were 735, 77 and 28 μM, respectively [25]. On the other hand, another in vitro study on the effect of PAMAM G4 on RBC lysis showed no evident haemolysis up to the high concentration of 50 µM (over 0.7 mg/ml, up to 24 h) [26].…”

Section: In Vitro Effects Of Pamam Dendrimers On Cellular Membranesmentioning

confidence: 95%

“…With the purpose of understanding the physiological and pathological changes occurring in this complex disease, animal models for diabetes are important research tools since they allow tight control of experimental conditions, which is almost impossible to achieve in any prospective study carried out on human populations [31,32]. Our first report [26] revealed that G4 poly(amido)amine dendrimers administered to rats with streptozotocindiabetes acted as scavengers for excess glucose, and greatly suppressed the accumulation of diabetesassociated products of protein glycation, glycoxidation or/ and oxidation. Hence, we further expected that improved glycaemic control, modulated in such a way, should also contribute to the normalized function and improved respiration capacity of heart and liver mitochondria in diabetes.…”

Section: Effect Of Pamam Dendrimers On Mitochondria Isolated Form Ratmentioning

confidence: 96%

“…Despite the rather promising finding that PAMAM G4 treatment suppressed diabetes-associated complications (hyperglycaemia, AGEs, AOPP, plasma cholesterol, triglycerides etc. ), the overall impact of this dendrimer on animal mortality was negative, indicating that such a beneficial effect cannot compromise PAMAM's G4 toxicity [26].…”

Section: Pamam Dendrimers In In Vivo Studiesmentioning

confidence: 99%

“…5.8 μM in circulating blood) we revealed much improved glycaemic control markers of diabetes, although PAMAM-treated rats, with better metabolic control of chronic hyperglycaemia, were more likely to die. Thus, mortality was significantly reduced in animals with higher blood glucose [26]. Accordingly, we hypothesized that the pathophysiological but natural modification might be non-enzymatic modification(s) of dendrimeric free surface amino groups.…”

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confidence: 99%

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PAMAM dendrimers — diverse biomedical applications. Facts and unresolved questions

Łabieniec

Watała

2009

Open Life Sciences

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Abstract:In this mini-review a number of novel outcomes, originating from studies in the field of PAMAM dendrimers, are presented and discussed. Owing to the multi-disciplinary nature of dendrimer chemistry it seems important to focus on the relevant topical research of PAMAM dendrimers, including their function, toxicity, surface modifications, and also possible new applications of these spherical polymers. We also consider the possibilities of specific functionalisation of PAMAM dendrimers -both novel ideas and those that have already been reported; as well as their cell-mediated effects (toxic and non-toxic). Then the reactivity of dendrimers' terminal groups, and their anticipated protective role against modifications of biomacromolecules, are discussed with regard to future developments in biomedical research. Mini-Review AbbreviationsAGEs -advanced glycation end products; AOPP -advanced oxidation protein products; RCI (S 3 *S 4 -1 ) -respiration control index (how mitochondria respond to ADP with enhanced oxygen consumption); ADP:O -coefficient of oxidative phosphorylation (how is respiration linked to phosphorylation); IC 50 -the concentration for which the 50% inhibition occurs; OPR -oxidative phosphorylation rate (how fast is ATP generated), MTT assay -standard colorimetric cytotoxicity test with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as a substrate; PAMAM -poly(amido)amine dendrimers; PEI -polyethyleneimine; S 3 -rate of oxygen uptake by mitochondria stimulated with ADP -state 3 (how fast is oxygen consumed by mitochondria in the presence of ADP and substrate); S 4 -rate of basal oxygen uptake by mitochondria -state 4 (how fast is oxygen consumed by mitochondria in the presence of substrate and the absence of ADP).

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Dendrimers as Powerful Building Blocks in Central Nervous System Disease: Headed for Successful Nanomedicine

Leiro

Santos

Lopes

et al. 2017

Adv Funct Materials

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Dendrimers have emerged as a powerful class of nanomaterials in the nanomedicine field due to their unique structural features: globular, welldefined, highly branched and controllable structure, nanosizescale, low polydispersity, and the presence of several terminal groups that can be functionalized with different ligands simulating the multivalency present in different biological systems. Although in its infancy, the application of dendrimers as therapeutics or theranostic tools in central nervous system (CNS) disorders is already significant and has opened promising avenues in the treatment of many conditions where the inherent "smartness" of the dendritic structures is being explored to effectively target the CNS. Here we present an overview of the past and future challenges of the use of dendrimers to respond to one of the ultimate challenges in the (nano)medicine field: to attain CNS repair and regeneration.

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PAMAM G4 dendrimers lower high glucose but do not improve reduced survival in diabetic rats

Cited by 33 publications

References 39 publications

Mitochondria Function in Diabetes – From Health to Pathology – New Perspectives for Treatment of Diabetes-Driven Disorders

Mitochondria Function in Diabetes – From Health to Pathology – New Perspectives for Treatment of Diabetes-Driven Disorders

PAMAM dendrimers — diverse biomedical applications. Facts and unresolved questions

Dendrimers as Powerful Building Blocks in Central Nervous System Disease: Headed for Successful Nanomedicine

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