Pamidronate Enhances Bacterial Adhesion to Bone Hydroxyapatite. Another Puzzle in the Pathology of Bisphosphonate-Related Osteonecrosis of the Jaw?

Kos, Marcin; Junka, Adam; Smutnicka, Danuta; Bartoszewicz, Marzenna; Kurzynowski, Tomasz; Gluza, Karolina

doi:10.1016/j.joms.2012.12.005

Cited by 44 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(vi) Since the exposed bone in MRONJ is colonized with bacteria of the oral cavity (e.g. actinomyces) and the microbial adhesion to bone appears to be altered under bisphosphonate therapy (Kos et al, 2013), a more or less active infection of the exposed bone can occur (Lesclous et al, 2009). Consequently, measures that disinfect the bone are needed to support the surgical treatment, which include ozone application (Agrillo et al, 2012), laser therapy , and in particular a prolonged antibiotic therapy (typically penicillin derivatives, alternatively tetracycline or clindamycin if patients are allergic to penicillin) (Montefusco et al, 2008).…”

Section: Surgical Appealmentioning

confidence: 99%

Treatment perspectives for medication-related osteonecrosis of the jaw (MRONJ)

Ristow

Otto

Troeltzsch

et al. 2015

Journal of Cranio-Maxillofacial Surgery

114

View full text Add to dashboard Cite

Section: Surgical Appealmentioning

confidence: 99%

Treatment perspectives for medication-related osteonecrosis of the jaw (MRONJ)

Ristow

Otto

Troeltzsch

et al. 2015

Journal of Cranio-Maxillofacial Surgery

114

View full text Add to dashboard Cite

“…To estimate the exact surface area of each HA disc, x-ray tomographic analysis was applied in a manner detailed by us previously. 12 …”

Section: Confirmation Of Tested Strains Ability To Form Biofilm On Hamentioning

confidence: 99%

Microbial Biofilms Are Able to Destroy Hydroxyapatite in the Absence of Host Immunity In Vitro

Junka¹,

Szymczyk²,

Smutnicka³

et al. 2015

Journal of Oral and Maxillofacial Surgery

Self Cite

View full text Add to dashboard Cite

Introduction It is widely thought that inflammation and osteoclastogenesis result in hydroxyapatite (HA) resorption and sequestra formation during osseous infections, and microbial biofilm pathogens induce the inflammatory destruction of HA. We hypothesized that biofilms associated with infectious bone disease can directly resorb HA in the absence of host inflammation or osteoclastogenesis. Therefore, we developed an in vitro model to test this hypothesis. Materials and Methods Customized HA discs were manufactured as a substrate for growing clinically relevant biofilm pathogens. Single-species biofilms of S.mutans, S.aureus, P.aeruginosa and C.albicans, and mixed-species biofilms of C.albicans + S.mutans were incubated on HA discs for 72 hours to grow mature biofilms. Three different non-biofilm control groups were also established for testing. HA discs were then evaluated by means of scanning electron microscopy, micro-CT metrotomography, x-ray spectroscopy and confocal microscopy with planimetric analysis. Additionally, quantitative cultures and pH assessment were performed. ANOVA was used to test for significance between treatment and control groups. Results All investigated biofilms were able to cause significant (P<0.05) and morphologically characteristic alterations in HA structure as compared to controls. The highest number of alterations observed was caused by mixed biofilms of C.albicans + S.mutans. S. mutans biofilm incubated in medium with additional sucrose content was the most detrimental to HA surfaces among single-species biofilms. Conclusion These findings suggest that direct microbial resorption of bone is possible in addition to immune-mediated destruction, which has important translational implications for the pathogenesis of chronic bone infections and for targeted antimicrobial therapeutics.

show abstract

“…The critical role of bacterial infection in the pathogenesis of MRONJ is also supported by clinical data showing that improvement of dental hygiene decreases the incidence of bisphosphonate-related ONJ in patients with multiple myeloma and metastatic cancer. 26 When extraction or bone surgery is required, the use of a chlorhexidine rinse is recommended before surgery and on a twice to 3 times daily basis until the surgical site has healed. 24,27,28 Several investigators recommend the use of prophylactic antibiotics when extracting teeth in patients on antiresorptive medication.…”

Section: Preventive Strategies After Commencement Of Antiresorptives mentioning

confidence: 99%

Preventive Strategies for Patients at Risk of Medication-related Osteonecrosis of the Jaw

Goodday

2015

Oral and Maxillofacial Surgery Clinics of North America

View full text Add to dashboard Cite

Pamidronate Enhances Bacterial Adhesion to Bone Hydroxyapatite. Another Puzzle in the Pathology of Bisphosphonate-Related Osteonecrosis of the Jaw?

Cited by 44 publications

References 21 publications

Treatment perspectives for medication-related osteonecrosis of the jaw (MRONJ)

Treatment perspectives for medication-related osteonecrosis of the jaw (MRONJ)

Microbial Biofilms Are Able to Destroy Hydroxyapatite in the Absence of Host Immunity In Vitro

Preventive Strategies for Patients at Risk of Medication-related Osteonecrosis of the Jaw

Contact Info

Product

Resources

About