Pan-cancer analysis combined with experiments explores the oncogenic role of spindle apparatus coiled-coil protein 1 (SPDL1)

Abstract

Background The function of spindle apparatus coiled-coil protein 1 (SPDL1) as a cancer-promoting gene has been reported in a number of studies. However, the pan-cancer analysis of SPDL1 is still lacking. Here, we performed this pan-cancer analysis to evaluate the expression and prognostic value of SPDL1 and gain insights into the association between SPDL1 and immune infiltration. Methods In this study, based on the datasets of The cancer genome atlas (TCGA), Gene expression omnibus (GEO), The Genotype-Tissue Expression (GTEx) and Clinical Proteomic Tumor Analysis Consortium (CPTAC), we used R4.1.0 software and the online tools, including TIMER2.0, GEPIA2, cBioPortal, Modbase, UALCAN, MEXPRESS, STRING, Ensembl, NCBI, HPA, Oncomine, PhosphoNET and the Kaplan-Meier plotter, to explore the potential oncogenic roles of SPDL1. The expression of SPDL1 was also further verified by immunohistochemistry (IHC) in lung adenocarcinoma (LUAD) tissues. Results SPDL1 was overexpressed in most tumors compared with adjacent normal tissues, and SPDL1 expression was significantly correlated with the prognosis in most tumor types. The main type of genetic mutation of SPDL1 was missense mutation and the frequency of R318Q/W mutation was highest (4/119). The expression of SPDL1 was closely associated with genomic instability. The SPDL1 phosphorylation levels in S555 was enhanced in ovarian cancer. The SPDL1 expression was positively correlated with the immune infiltration of CD8+ T-cells and cancer-associated fibroblasts (CAFs) in most of the tumor types. Nuclear division, organelle fission and chromosome segregation were involved in the functional mechanisms of SPDL1. Conclusions These findings suggested that SPDL1 might serve as a biomarker for poor prognosis and immune infiltration in cancers, shedding new light on therapeutics of cancers.

Proteomic Changes in the Cytoplasmatic Fraction of Weaned Piglets Liver and Kidney Under Antioxidants and Mycotoxins Diets

“…This included down-regulation of spindle apparatus coiled-coil protein 1 (SPDL1), and fatty acid binding protein 1 (FABP1), along with the up-regulation of OAS1 and REN, suggesting complex interactions between antioxidants and mycotoxins. In the kidney cytoplasmatic fraction, SPDL1 contributes to the maintenance of genomic stability during cell division (Song et al 2022), ensuring proper development and function of renal cells. FABP1, primarily expressed in the liver but also found in renal proximal tubular cells, is involved in the PPAR signalling pathway ( Figure S25 ), through uptake, transport, and utilization of fatty acids, which are essential for energy production and membrane synthesis in renal cells (Wang et al 2015).…”

“…stability during cell division(Song et al 2022), ensuring proper development and function of renal cells. FABP1, primarily expressed in the liver but also found in renal proximal tubular cells, is involved in the PPAR signalling pathway (FigureS25), through uptake, transport, and utilization of fatty acids, which are essential for energy production and membrane synthesis in renal cells(Wang et al 2015).The down-regulation of SPDL1 and FABP1 expression might indicate disruptions in the cell division processes and lipid metabolism, respectively, while the up-regulation of REN suggests potential involvement in the urea cycle.…”

Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma

“…GSVA showed that a number of pathways associated with malignancy were substantially upregulated in the low-risk group. These included hypoxia (43)(44)(45), the p53 pathway (44,46), KRAS signaling (47,48), TGF signaling MTOR signaling pathways (49,50), mitotic spindle (51), and other processes. All of the above data suggest that many pathways may be involved in the underlying mechanisms responsible for the poor prognosis of LUAD patients.…”

Comprehensive analysis of single-cell and bulk RNA-sequencing data identifies B cell marker genes signature that predicts prognosis and analysis of immune checkpoints expression in head and neck squamous cell carcinoma

“…According to the previous study, the pathological assessment of staining was performed using the CPS, and CPS ≥1 was considered as positive [ 32 , 33 ]. We used the semi-quantitative scoring method according to a previous study by multiplying the score of staining intensity and the score of positive cells for FTH1 [ 34 ]. The low expression was defined as the scores from 0 to 6, and the high expression was defined as the scores from 7 to 12.…”