Panax notoginseng stimulates alkaline phosphatase activity, collagen synthesis, and mineralization in osteoblastic MC3T3-E1 cells

Abstract

Total Panax notoginseng saponin (PNS) has been extensively used to treat a variety of diseases, such as bone fractures, soft tissue injuries, etc. In this study, mouse calvaria-original osteoblastic MC3T3-E1 cells were cultured in various concentrations of PNS (0.005-5 mg/mL) during the period (1, 5, 14, and 23 d). At the endpoint, the osteogenic capacity of MC3T3-E1 cells was investigated by measuring the alkaline phosphatase (ALP) activity, the deposited calcium, and the expression of osteogenic-related markers, including bone collagen type 1 (Col1) and osteocalcin (OCN). Compared with all groups in each period, the most pronounced effect was observed at the concentration range between 0.05 and 0.5 mg/mL (P < 0.05) and the cell proliferation with PNS treatment was found during the whole osteogenic period. Moreover, cellular ALP activity with PNS was increased during 7, 14, and 21 d and cell mineralization with PNS was enhanced in 14 and 21 d. Furthermore, the differentiation markers Col1 and OCN increased in the PNS-treated cells. Our work suggests that PNS may stimulate the osteogenesis process which contains osteoblastic proliferation, differentiation, and mineralization by increasing cellular ALP activity, extracellular matrix mineralization, and osteoblast-associated molecules in the osteoblasts.

Quercetin Stimulates Bone Marrow Mesenchymal Stem Cell Differentiation through an Estrogen Receptor-Mediated Pathway

“…ALP [ 30 ] is an early osteogenic marker and plays an important role in bone formation. A peak in ALP activity is indicative of differentiation [ 31 , 32 ]. We quantified ALP activity to evaluate the effect of quercetin on BMSC osteogenic differentiation.…”

Panax notoginseng Saponin Promotes Bone Regeneration in Distraction Osteogenesis via the TGF-β1 Signaling Pathway

“…PNS has previously been purported to exhibit pro-bone metabolism, neuroprotective, hepatoprotective, renoprotective, antitumor, cardioprotective, antidiabetic, and other beneficial effects, in addition to being safe and convenient to use [ 30 ]. Prior evidence also suggests that PNS and its main bioactive component, NGR1, can stimulate the proliferation, differentiation, and mineralization of osteoblasts through the enhancement of cellular ALP activity, ECM mineralization, and osteoblast-associated gene expression [ 31 , 32 ]. The results of the present study further confirmed that PNS administration was sufficient to enhance osteogenesis in a rabbit model of DO, with reductions in PPM1A expression together with the upregulation of TGF- β 1/Smad signaling pathway genes (T β R-II, SMAD2/3), potentially favoring osteogenic differentiation and DO regeneration.…”

<p>Focus on Notoginsenoside R1 in Metabolism and Prevention Against Human Diseases</p>

“…Recently, it has been demonstrated that PN saponins promote osteogenesis, including proliferation, differentiation, and mineralization activity in osteoblasts. 77 Accordingly, NG-R1 significantly induces bone development by inhibition of RNAKL-mediated MAPK and NF-κB signaling pathways and suppression of osteoclastogenesis and bone resorption. 15 In addition, NG-R1 may significantly induce the expression of Runx-2 and Osx as well as the activity of ALP in MC3T3-E1 cells dose-dependently.…”