Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride

Inhibition of transforming growth factor β decreases pancreatic fibrosis and protects the pancreas against chronic injury in mice

“…[2][3][4][5][6][7] Many studies have demonstrated the presence of TGF-b1 in the form of either protein or message, in the fibrotic tissues of human chronic pancreatitis and animal models. [8][9][10]23,32 Van Laethem et al 33 demonstrated that repeated injections of recombinant TGF-b into mice after recurrent episodes of acute pancreatitis led to pancreatic fibrosis. On the other hand, Menke et al 13 have demonstrated that inhibition of TGF-b1 by TGF-b1-neutralizing antibody causes partial reduction of the accumulation of ECM (collagen type I and III, and fibronectin) during regeneration from the cerulein-induced acute pancreatitis.…”

Bone marrow-derived pancreatic stellate cells in rats

“…shown to initiate dose-and strain-dependent pancreatic inflammatory reactions in rats [17,31]. Chimeric BN/LEW rats responded to 4-6 mg per kg body weight of DBTC with an acute interstitial pancreatitis followed by pancreatic regeneration during 4 weeks.…”

“…For this approach, a respective amount of DBTC was applied as described previously [31]. Briefly, DBTC was dissolved in a mixture of glycerol/ethanol (3:2) followed by injection into the tail vein in a maximum volume of 200 µl per animal.…”

Gene therapy for pancreatitis pain

“…14 Elevated serum a-amylase, lipase and C-reactive protein levels serve as biochemical markers of acute pancreatitis. 15,16 Acute pancreatitis ranges from mild edematous conditions that usually heal without intervention to severe hemorrhagic necrotizing inflammation that is often fatal over a period of days as patients succumb to abdominal sepsis and multiorgan failure. 17 The level of pain experienced by these patients is directly linked to the decreased pancreatic functioning and increased length of stay during hospitalizations.…”