Pancreatic islet cell hormones distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell.

Erlandsen, S L; Hegre, Orion D.; Parsons, J. A.; McEvoy, Robert; Elde, Robert

doi:10.1177/24.7.60437

Cited by 174 publications

(65 citation statements)

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“…However, most non-pcells remained near the exterior surface of the islet when the invaginations were taken into consideration. Therefore, the distribution of the individual cell types within human islets was similar to the rat islets; these cells are best described as modified mantle islets (26).…”

Section: Resultsmentioning

confidence: 99%

“…The predominance of this organizational pattern can only be appreciated in the threedimensional reconstructions. 6) Although the human islet has been described as a modified mantle islet (26), the preferential distribution of 8-cells slightly more exteriorly than acells has not been described. 7) Individual secretory granules were resolved in human a-cells in a monolayer culture.…”

Section: Discussionmentioning

confidence: 99%

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Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy

Brelje

Sorenson

1989

Diabetes

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We present a new technique for analyzing the three-dimensional structure of intact isolated islets of Langerhans. Adult rat and human islets were stained with whole-mount immunofluorescence techniques and optically sectioned with a confocal microscope. This has several advantages over traditional methods: 1) the technical difficulties in serial sectioning and handling the large numbers of sections are avoided, 2) optical sectioning by confocal microscopy gives improved resolution and strongly suppresses light from out-of-focus structures, and 3) entire islets can be rapidly imaged for the presence of positive staining. This new technique should facilitate the study of the three-dimensional structure of islets of Langerhans. Diabetes 38:808-14,1989 T he three-dimensional structure of islets of Langerhans is important in understanding the regulatory mechanisms involved in controlling the different islet cell types. Since the first immunofluorescent localization of insulin to p-cells by Lacy and Davies (1,2), glucagon to the a-cell by Baum et al. (3), somatostatin to the 5-cell by several investigators (4-7), and pancreatic polypeptide (PP) to a distinct cell type by Larsson and coworkers (8-10), fluorescence microscopy has been an important technique in investigating the structure of islets.However, a severe limitation with conventional fluorescence microscopy has been fluorescent background resulting from out-of-focus structures, which reduces image contrast and therefore limits the amount of information that can be obtained from in-focus structures. To overcome this limitation, it has been necessary to examine thin sections of

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy

Brelje

Sorenson

1989

Diabetes

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“…Elution has been performed with varying degrees of success using glycine buffer (7), dimethylformamide (18), glycine and dimethylformamide in combination with electrophoresis (19), KMnO 4 -H 2 SO 4 (20), and HCl (21). Shorter denaturation at a higher temperature (130°C) can be highly efficient if the tissue is properly shielded by immersion in glycerin for 5-7 days (22).…”

Section: Figmentioning

confidence: 99%

Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei

et al. 2001

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Background: Visualization of more than one antigen by multicolor immunostaining is often desirable or even necessary to explore spatial and temporal relationships of functional significance. Previously presented staining protocols have been limited to the visualization of three or four antigens. Methods: Immunofluorescence staining was performed both on slices of formalin-fixed tissue and on cells in culture. Images of the stained material were recorded using digital imaging fluorescence microscopy. The primary and secondary antibodies, as well as the fluorophores, were thereafter removed using a combination of denaturation and elution techniques. After removal of the fluorescence stain, a new immunofluorescence staining was performed, visualizing a new set of antigens. The procedure was repeated up to three times. A method for

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“…In the present study, no gastrin immunoreactive cells were detected in the normal islets of humans, dogs or guinea pigs. Until some years ago, D cells in islets were believed to produce gastrin (4,7,23), but now it is known that they secrete a somatostatin (14,30). Gastrinomas are second to insulinomas in frequency, and most of them are in the pancreas (21), so that gastrin cells have been looked for in normal islets by many investigators.…”

Section: Resultsmentioning

confidence: 99%

Enzyme-immunohistochemical studies of argyrophil cells in normal islets and islet cell tumors.

Takahashi

Nakagawa

Otsuki

et al. 1981

Acta Histochem. Cytochem

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Pancreatic islet cell hormones distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell.

Cited by 174 publications

References 0 publications

Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy

Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy

Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei

Enzyme-immunohistochemical studies of argyrophil cells in normal islets and islet cell tumors.

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