Pancreatic islet transplantation for treating diabetes

Matsumoto, Shinichi; Noguchi, Hirofumi; Yonekawa, Yukihide; Okitsu, Teru; Iwanaga, Yasuhiro; Xiao-ling, Liu; Nagata, Hideo; Kobayashi, Naoya; Ricordi, Camillo

doi:10.1517/14712598.6.1.23

Cited by 34 publications

(31 citation statements)

References 182 publications

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“…Recently We introduced a large amount of DI for maintaining patency of pancreatic duct for the collagenase delivery as Sutherland et al demonstrated that islet autotransplantation is much more effective than allotransplantation (22) well as protecting islets and exocrine tissue from cold ischemic injury (12). Actually, DI could significantly and our results with islet autotransplantation support their observation.…”

Section: Discussionsupporting

confidence: 67%

“…Approximately 1 ml/g pancreas of ET-Kyoto soluThe quality of pancreatic tissue retrieved from CP tion (Otsuka Pharm Factory Inc., Naruto, Japan) was patients undergoing total or partial pancreatectomy is administered intraductally before preservation in oxyinferior from the pancreas retrieved from deceased genated perfluorodecalin (11,12). For the UW group, the donors.…”

Section: The First Demonstration That Islets Isolated Frommentioning

confidence: 99%

“…DI+TLM was originally developed for islet isothree cases of the DI+TLM group. In one case in the lation from a non-heart-beating donor (12) and applied DI+TLM group, the islets were purified with a continufor living donor islet transplantation (13) in Japan. Curous density gradient using Biocoll in a chilled apheresis rently this technique is also applied for islet isolation system (COBE 2991 Cell Processor, CaridianBCT, from heart-beating brain-dead donor pancreas (5,10,11).…”

Section: The First Demonstration That Islets Isolated Frommentioning

confidence: 99%

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Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic Pancreatitis

Naziruddin

Matsumoto²,

Noguchi³

et al. 2012

Cell Transplant

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Total or partial pancreatectomy followed by autologous islet transplantation is a therapeutic option for the treatment of refractory chronic pancreatitis (CP). Maximization of islet yields from fibrotic and inflamed organs is crucial for prevention of posttransplant diabetes. We adapted technical advancements developed for islet allotransplantation toward islet autotransplantation. Eight patients (two men, six women; ages 24-58 years) underwent total (n = 7) or partial (n = 1) pancreatectomy for the treatment of CP refractory to maximal medical management. Pancreata were preserved in UW solution (UW group) in initial three cases and the last five pancreata were preserved with pancreatic ductal injection followed by ET-Kyoto/oxygenated PFC solutions (DI+TLM group). Islets were isolated by modified Ricordi method and were purified only in one case. All islet infusions were performed under general anesthesia via direct vein injection into the portal venous system with pressure monitoring. Total islet yields (129,314 ± 51,627 vs. 572,841 ± 116,934 IEQ, p < 0.04), islet yield/pancreas weight (1,233 ± 359 vs. 6,848 ± 847 IEQ/g, p < 0.003), and islet yield/patient body weight (1,951 ± 762 vs. 7,305 ± 1,531 IEQ/kg, p < 0.05) were significantly higher in the DI+TLM group when compared to the UW group. Pellet size was also higher (5.3 ± 0.3 vs. 13.5 ± 3.4 ml) in the DI+TLM group, suggesting that this method of preservation effectively protected pancreatic tissue against autolysis. First month posttransplant basal C-peptide and the secretory unit of islet transplant objects (SUITO) index were also higher in the DI+TLM group when compared to the UW group (2.0 ± 0.3 vs. 1.4 ± 0.4 ng/ml and 42.6 ± 12.7 vs. 14.6 ± 5.6, respectively). There were no technical complications related to the infusion. Our results suggest that higher islet yields can be achieved even from chronically inflamed and fibrotic organs using DI+TLM. The techniques applied for islet isolations from normal pancreata are showing promise for fibrotic pancreata from CP patients.

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Section: Discussionsupporting

confidence: 67%

Section: The First Demonstration That Islets Isolated Frommentioning

confidence: 99%

Section: The First Demonstration That Islets Isolated Frommentioning

confidence: 99%

See 1 more Smart Citation

Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic Pancreatitis

Naziruddin

Matsumoto²,

Noguchi³

et al. 2012

Cell Transplant

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“…32 Liberase HI (Roche, Indianapolis, Ind) or collagenase NB with neutral proteases (Serva Electrophoresis GMbH, Heidelberg, Germany) was continuously infused into the pancreas graft. 33 For pancreas digestion, we used the Ricordi method. 34 If pellet volume was larger than 15 mL, islet purification was performed using the COBE 2991 cell processor (CaridianBCT, Inc, Lakewood, Colo) with continuous density gradient.…”

Section: Islet Preparation and Evaluationmentioning

confidence: 99%

Implication of Pancreatic Image Findings in Total Pancreatectomy With Islet Autotransplantation for Chronic Pancreatitis

Takita¹,

Naziruddin²,

Matsumoto³

et al. 2011

Pancreas

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“…However, because we have very limited number of such donors in Japan, it is not possible to perform islet transplantation with brain-dead heartbeating donors. In order to initiate an islet transplantation program in Japan, we need to seek new donor resources (7).…”

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confidence: 99%