Pancreatic islets microencapsulation with Polylactide-Co-Glycolide

Abalovich, Adrian; Jatimliansky, C; Diegex, E; Arias, Myriam; Altamirano, A; Amorena, Carlos; Martínez, Bárbara; Nacucchio, Marcelo Carlos

doi:10.1016/s0041-1345(01)01918-2

Cited by 18 publications

(10 citation statements)

References 2 publications

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“…55 Microencapsulation of pancreatic islets has been discerned by interfacial precipitation of poly(lactic glycolic acid) (PLGA), although normal glycemia is not completely restored in transplanted diabetic rats. 131 Liquid-core capsules by gelation (ionotropic or complex coacervation)…”

Section: Interfacial Precipitation (Or Simple Coacervation)mentioning

confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

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Cell encapsulation in microcapsules allows the in situ delivery of secreted proteins to treat different pathological conditions. Spherical microcapsules offer optimal surface-to-volume ratio for protein and nutrient diffusion, and thus, cell viability. This technology permits cell survival along with protein secretion activity upon appropriate host stimuli without the deleterious effects of immunosuppressant drugs. Microcapsules can be classified in 3 categories: matrix-core/shell microcapsules, liquid-core/shell microcapsules, and cells-core/shell microcapsules (or conformal coating). Many preparation techniques using natural or synthetic polymers as well as inorganic compounds have been reported. Matrix-core/shell microcapsules in which cells are hydrogel-embedded, exemplified by alginates capsule, is by far the most studied method. Numerous refinement of the technique have been proposed over the years such as better material characterization and purification, improvements in microbead generation methods, and new microbeads coating techniques. Other approaches, based on liquid-core capsules showed improved protein production and increased cell survival. But aside those more traditional techniques, new techniques are emerging in response to shortcomings of existing methods. More recently, direct cell aggregate coating have been proposed to minimize membrane thickness and implants size. Microcapsule performances are largely dictated by the physicochemical properties of the materials and the preparation techniques employed. Despite numerous promising pre-clinical results, at the present time each methods proposed need further improvements before reaching the clinical phase.

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Section: Interfacial Precipitation (Or Simple Coacervation)mentioning

confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

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“…However, their use is limited by the hydrophobic nature of these materials (which reduces their biocompatibility) and the release of proinflammatory acidic by‐products during their degradation (Gunatillake & Adhikari, ). Synthetic scaffolds have been used alone in several in vitro and in vivo islet and islet‐like transplantation approaches in animals, mainly at extrahepatic sites, both as micro‐ (Abalovich et al, ; Jang, Lee, Park, & Byun, ; Rengifo, Giraldo, Labrada, & Stabler, ; Teramura & Iwata, ; Wilson, Cui, & Chaikof, ) and macro‐encapsulation devices (Blomeier et al, ; Dufour et al, ; Frei, Li, Jiang, Buchwald, & Stabler, ; Gibly, Zhang, Lowe, & Shea, ; Hill et al, ; Jiang et al, ; Juang, Bonner‐Weir, Ogawa, Vacanti, & Weir, ; G. Mao, Chen, Bai, Song, & Wang, ; Pedraza et al, ; Rios et al, ; Salvay et al, ; C. Song et al, ; Weaver et al, ). Most of these studies have reported an improvement in islet engraftment and a decreased immune response.…”

Section: Tissue Engineering In Islet Transplantationmentioning

confidence: 99%

Tissue‐engineering approaches in pancreatic islet transplantation

Pérez-Basterrechea

Esteban

Vega

et al. 2018

Biotech & Bioengineering

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Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ transplantation. By using a variety of strategies used in tissue engineering and regenerative medicine, pancreatic islets have been successfully introduced in host patients with different outcomes in terms of islet survival and functionality, as well as the desired normoglycemic control. Here, we describe and discuss those strategies to transplant islets together with different scaffolds, in combination with various cell types and diffusible factors, and always with the aim of reducing host immune response and achieving islet survival, regardless of the site of transplantation.

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“…This is in large part due to the challenges associated with PLGA, specifically the pH drop that occurs during degradation, which can result in aggregation of loaded proteins and elicit an immune response (71,72). Porcine islets encapsulated in PLGA microspheres and transplanted into the abdominal cavity of diabetic rats reduced blood glucose levels compared to control subjects (73), however the animals failed to return to normoglycemia.…”

Section: Biosynthetic Materials For Islet Deliverymentioning

confidence: 99%

Bio-synthetic materials for immunomodulation of islet transplants

Foster

Garcı́a

2017

Advanced Drug Delivery Reviews

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Clinical islet transplantation is an effective therapy in restoring physiological glycemic control in type 1 diabetics. However, allogeneic islets derived from cadaveric sources elicit immune responses that result in acute and chronic islet destruction. To prevent immune destruction of islets, transplant recipients require lifelong delivery of immunosuppressive drugs, which are associated with debilitating side effects. Biomaterial-based strategies to eliminate the need for immunosuppressive drugs are an emerging therapy for improving islet transplantation. In this context, two main approaches have been used: 1) encapsulation of islets to prevent infiltration and contact of immune cells, and 2) local release of immunomodulatory molecules from biomaterial systems that suppress local immunity. Synthetic biomaterials provide excellent control over material properties, molecule presentation, and therapeutic release, and thus, are an emerging platform for immunomodulation to facilitate islet transplantation. This review highlights various synthetic biomaterial-based strategies for preventing immune rejection of islet allografts.

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Pancreatic islets microencapsulation with Polylactide-Co-Glycolide

Cited by 18 publications

References 2 publications

Progress technology in microencapsulation methods for cell therapy

Progress technology in microencapsulation methods for cell therapy

Tissue‐engineering approaches in pancreatic islet transplantation

Bio-synthetic materials for immunomodulation of islet transplants

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