Pancreatic PYY but not PPY expression is responsive to short-term nutritional state and the pancreas constitutes the major site of PYY mRNA expression in chickens

Abstract

HighlightsChicken and quail PYY mRNA sequences evidenced.Pancreas identified as the major site of PYY mRNA expression in chickens.Peak of intestinal expression evidenced around the distal jejunum.Ontogenic development of gradient pancreatic expression of chicken PYY and PPY reported.Chicken pancreatic PYY shown to increase in response to short-term nutritional state.

Gastrointestinal distribution of chicken gastrin-cholecystokinin family transcript expression and response to short-term nutritive state

“…CCK is heavily implicated in the short-term satiety response in vertebrate species ( Havel, 2001 , Murashita et al, 2007 , Moran, 2009 , Gibbons et al, 2016 , Honda et al, 2017 , Volkoff et al, 2017 ). The circulating peptide longevity is known to be very short ( Liddle et al, 1985 ), although a delay in transcriptional response might have been expected, as observed for the satiety factor peptide YY in chickens ( Reid et al, 2017 ). CCK did not alter significantly in response to short-term satiety state within the scope of the fed/fasted experiment (Section 2.1.2 .…”

“…BLASTN (NCBI) returned no unintended chicken targets for either probe. Primers for quantification of LBR, YWHAZ and NDUFA1 as reference genes were described previously ( Reid et al, 2017 ). Sigma-Aldrich UK supplied all oligonucleotide primers and probes.…”

Weighted single‐step genome‐wide association study and pathway analyses for feed efficiency traits in Nellore cattle

“…The neuropeptide signalling pathway (GO:0007218) was associated with growth and feed utilization traits of Japanese Black cattle (Okada et al., 2018), suggesting that this pathway may affect FE. Among the genes harboured in these pathways, we highlighted the PYY , HCRT , HCRTR , NMB , MLNR , NPBWR1 , PPY , SPX , OPRK1 and PENK genes, which are related to feeding behaviour, satiety and amount of food consumed (Arora, 2006; Hoggard, Bashir, Cruickshank, Miller, & Speakman, 2007; Martín‐García et al., 2011; McGregor, Wu, Barber, Ramanathan, & Siegel, 2011; Reid et al., 2017; Reyes‐Alcaraz et al., 2016; Sakuraia, 2013; Takahashi, Rikimaru, Komatsu, Uemoto, & Suzuki, 2014; Tyree, Borniger, & Lecea, 2018; Xu et al., 2013).…”

“…The HCRT and PPY genes have shown a change in circulation within minutes to hours after feeding (Graaf, Blom, Smeets, Stafleu, & Hendriks, 2004; Reid et al., 2017; Tyree et al., 2018). The action of these genes is mediated via leptin and insulin, leading to sensation satiety and acting in feed intake, energy homeostasis and balance (Graaf et al., 2004; Reid et al., 2017; Tyree et al., 2018).…”

Acute restraint stress does not alter corticosteroid receptors or 11β-hydroxysteroid dehydrogenase gene expression at hypothalamic–pituitary-adrenal axis regulatory sites in captive male white-crowned sparrows (Zonotrichia leucophrys gambelii)

“…All assays used Brilliant 111 Ultra-fast SYBR Green qPCR Master mix (Agilent Technologies 60083) which was read on an Agilent Mx3005p qPCR system and processed with MxPro software (Agilent Technologies) as previously described (Reid, Wilson et al 2017). In brief, a 20μL total reaction volume per sample was generated by combining 10μL SYBR Green, 8μL cDNA, 0.4μL 20μM forward primer, 0.4 μL 20 μM reverse primer, 0.3μL 1/500 dilution of ROX reference dye solution, and 0.9μL Milli-Q H2O.…”