Pancytopenia revealing acute brucellosis

Lahlou, Yassine Ben; Benaissa, Elmostafa; Maleb, Adil; Chadli, Mariama; Elouennass, Mostafa

doi:10.1016/j.idcr.2020.e01037

Cited by 6 publications

(8 citation statements)

References 10 publications

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“…However, a small proportion of patients may encounter treatment limitations due to individual factors such as immune system deficiencies or dialysis [ 16 , 17 ]. In previous reports, patients with brucellosis and pancytopenia or other hematological complications generally achieved complete recovery with antibiotic treatment, including regimens comprising rifampicin and doxycycline or streptomycin [ 5 , 7 , 8 , 18 , 19 ]. However, the case described in this report experienced worsening of symptoms and did not sufficiently respond to conventional antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

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Application of immunomodulatory therapy in a human brucellosis patient with pancytopenia: A case report

Zhang

Dang

et al. 2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

“…Based on previous case reports, the mechanism underlying the association between brucellosis and pancytopenia remains unclear. Currently, it is believed to be potentially associated with splenomegaly, impaired phagocytic function, compromised immune function, and bone marrow suppression [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Application of immunomodulatory therapy in a human brucellosis patient with pancytopenia: A case report

Zhang

Dang

et al. 2023

Heliyon

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“…canis and B. suis [29, 30]. Due to the subversion of immune recognition of host and immuno-modulatory responses, the disease progression slowly enters into sub-stages of acute, sub-acute and chronic disease presentation [31].…”

Section: Introductionmentioning

confidence: 99%

“…Since B. melitensis is highly contagious and leads to the most invasive and severe brucellosis infection in humans, followed by other important Brucella species, namely B. abortus, B. canis and B. suis [29,30]. Due to the subversion of immune recognition of host and immuno-modulatory responses, the disease progression slowly enters into sub-stages of acute, sub-acute and chronic disease presentation [31].…”

Section: Introductionmentioning

confidence: 99%

Immunoassay-based evaluation of rOmp28 protein as a candidate for the identification of Brucella species

Hans

Thavaselvam

2023

Journal of Medical Microbiology

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Introduction. Brucellosis is an important bacterial zoonosis, re-emerging as a serious public health concern in developing countries. Two major species, Brucella melitensis and Brucella abortus , cause recurrent facile infection in human. Therefore, rapid and accurate diagnosis for early disease control and prevention is needed in areas with low disease burden. Hypothesis. This study evaluated the sandwich enzyme-linked immunosorbent assay (ELISA) (S-ELISA) immunoassay for potential use of whole-cell (WC) and recombinant outer-membrane protein (rOmp28)-derived IgG polyclonals in sensitive detection of Brucella . Aim. Immunoassay-based WC detection of Brucella species in important sub-clinical matrices at lower limits of detection. Methodology. We purified recombinant rOmp28 with Ni–NTA gel affinity chromatography and produced IgG polyclonal antibodies (pAbs) using BALB/c mice and New Zealand white female rabbits against different antigens (Ags) of Brucella . Checkerboard sandwich ELISA and P/N ratio (optical density of ‘P’ positive test sample to ‘N’ negative control) were used for evaluation and optimization of the study. The pAbs were characterized using Western blot analysis and different matrices were spiked with WC Ag of Brucella . Results. Double-antibody S-ELISA was developed using WC Ag-derived rabbit IgG (capture antibody at 10 µg ml−1) and rOmp28-derived mice IgG (detection antibody at 100 µg ml−1) with a detection range of 102 to 108 cells ml−1 and a limit of detection at 102 cells ml−1. A P/N ratio of 1.1 was obtained with WC pAbs as compared to 0.6 and 0.9 ratios with rOmp28-derived pAbs for detecting B. melitensis 16M and B. abortus S99, respectively. An increased P/N ratio of 4.4 was obtained with WC Ag-derived rabbit IgG as compared to 4.2>4.1>2.4 ratios obtained with rabbit IgGs derived against cell envelope (CE), rOmp28 and sonicated antigen (SA) of Brucella with high affinity for rOmp28 Ag analysed on immunoblots. The rOmp28-derived mice IgG revealed two Brucella species at P/N ratios of 11.8 and 6.3, respectively. Upon validation, S-ELISA detected Brucella WCs in human whole blood and sera samples with no cross-reactivity to other related bacteria. Conclusion. The developed S-ELISA is specific and sensitive in early detection of Brucella from different matrices of clinical and non-clinical disease presentation.

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“…It is a widespread disease in the world and one of the most important zoonoses worldwide in many countries, with 500 thousand cases reported each year according to WHO [10]. This pathology remains as endemic in many countries [11]. In Africa little data is available on human brucellosis [12].…”

Section: Introductionmentioning

confidence: 99%

New approach needed for diagnosis of human brucellosis in Morocco

et al. 2021

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Human brucellosis is an infectious disease, caused by different species of the genus Brucella. It is a mandatory notifiable disease in most countries, including Morocco, and thus requires special vigilance on the part of health agencies. Prevention of this disease is based on surveillance and prevention of risk factors. Its management is based on serological or other diagnostic tests. The National Laboratory of Epidemic Diseases received 17 blood samples of human origin for notified cases of brucellosis in a region of southern Morocco. Biological confirmation of these samples was performed by Rose Bengal test and ELISA IgM test. The results showed a positivity rate of 88% proving the important role of serological tests in the diagnosis of brucellosis. Other rapid and efficient techniques are becoming essential for the confirmation of cases, taking into account the advanced biosafety procedures for these highly infectious pathogens.

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Pancytopenia revealing acute brucellosis

Cited by 6 publications

References 10 publications

Application of immunomodulatory therapy in a human brucellosis patient with pancytopenia: A case report

Application of immunomodulatory therapy in a human brucellosis patient with pancytopenia: A case report

Immunoassay-based evaluation of rOmp28 protein as a candidate for the identification of Brucella species

New approach needed for diagnosis of human brucellosis in Morocco

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