Pandoraea pnomenusa Isolated from an Australian Patient with Cystic Fibrosis

Abstract

Pandoraea species are considered as emerging pathogens in people with cystic fibrosis (CF). The contribution of these organisms to disease progression in CF patients is not fully understood owing in large measure to the scant reports in clinical and research literature describing their colonization of CF patients and their associated virulence determinants. In an effort to increase awareness and evidence for Pandoraea spp. infection in people with CF, and to stimulate research aimed at unraveling the pathogenic properties of Pandoraea, we report a case of a 26-year-old Australian (Tasmanian) man with CF who was chronically infected with Pandoraea pnomenusa for at least one year prior to his death from respiratory failure. In addition, we describe for the first time evidence suggesting that this bacterium is a facultative anaerobe and report on the availability of a whole genome sequence for this organism. To the best of our knowledge, this report represents only the second clinical case study of P. pnomenusa infection in the world, and the first in an Australian CF patient.

Pneumonia due to Pandoraea Apista after evacuation of traumatic intracranial hematomas:a case report and literature review

“…Pandoraea species belongs to the β-subclass of the Proteobacteria , which contains a group of Gram-negative bacilli that are aerobic or facultative anaerobic (e.g. P. pnomenusa reported by Ambrose et al [3]), do not form spores, do not reduce nitrate, do not ferment lactose, and rely on flagellar movement. Growth is observed at 30 °C and 37 °C.…”

“…The main sources of Pandoraea species in the environment including soil, animal feces, water, and even powdered milk [8]. The clinical species were mainly isolated from respiratory specimens of cystic fibrosis (CF) patients [1, 3, 7, 9–24]. To date, cases of Pandoraea species caused colonization or infection have been reported all over the world, including USA [1, 7, 12, 25], Denmark [1, 10, 26], Germany [13], France [18, 19], Ireland [26], Argentina [20, 24], Spain [16, 17, 27, 28], Australia [3, 14, 15], Canada [1], UK [1, 9, 22], China [29], Belgium [1], Brazil [1], Sweden [1] (See at Fig.…”

“…With less information contributed by Pandoraea species in the database used might limit the discriminatory power of this method. Misidentification in strains-level has been reported [3]. MALDI-TOF MS is a promising approach, but more specific information is needed to update its database for accurate confirmation of bacteria that is less common.…”

Isolation and identification of Pandoraea spp. From bronchoalveolar lavage of cystic fibrosis patients in Iran

“…are present in the lung of CF patients, lung infections, and oral and dental infections. According to the studies conducted to data on patients with CF and lung infection diseases, opportunistic pathogens that have been involved in causing diseases including Pseudomonas , Burkholderia and Pandoraea species, have been isolated and identified [29, 36–38].…”

“…In 2008, Karher et al examined the pathogenic and genetic characteristics of Pandoraea species in lung epithelial cells. In this study, 17 Pandoraea bacteria were isolated including 5 species: P. apista , P. pnomenusa , P. sputorum, P. pulmonicola and P. norimbergensis [38].…”