Panel Filmarray GI® en la detección de patógenos entéricos en deposiciones: experiencia preliminar

Farfán, Mauricio J.; Piemonte, Paula; Labra, Yohanna; Henríquez, J. A. Medina; Candia, Enzo; Torres, Juan Pablo

doi:10.4067/s0716-10182016000100016

Cited by 11 publications

(19 citation statements)

References 8 publications

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“…The high frequency of co-detection in our study, particularly among the diarrheagenic E. coli pathotypes, is similar to prior studies using the FilmArray ® PCR in which co-detections were reported in 21–48% of positive samples 9,12,21–23. Although co-detections in diarrheal diseases will likely be more commonly recognized with the increasing use of multiplexed molecular assays, whether such detections represent true coinfections of viable organisms or colonizers cannot be determined.…”

Section: Discussionsupporting

confidence: 85%

“…In our study using multiplex PCR, 14 different pathogens were identified with an overall detection rate of 62.7%. This detection rate exceeds traditional laboratory detection methods2 and compares favorably to prior studies utilizing the FilmArray ® PCR in which detection rates ranged from 33% to 48% 9,12,21–23. Thus, the breadth of pathogens surveyed and ease of use of this PCR platform make it well suited for epidemiologic studies, particularly in settings such as ours in which the traditional suite of culture media types, immunoassays, and uniplex PCR protocols used to survey a similarly broad range of pathogens would be difficult to implement due to limited human and laboratory resources.…”

Section: Discussionsupporting

confidence: 64%

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Multiplex Polymerase Chain Reaction for Detection of Gastrointestinal Pathogens in Migrant Workers in Qatar

Humphrey

Ranbhise

Ibrahim

et al. 2016

The American Society of Tropical Medicine and Hygiene

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The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015–2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray® Gastrointestinal PCR). A target pathogen was identified in 62.7% of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6% of subjects, followed by Salmonella (22.2%), enteroaggregative E. coli (15.1%), Giardia lamblia (9.5%), and enterotoxigenic E. coli (8.7%). Multiple pathogens were identified in 49.3% of positive stool samples. In a multivariable analysis, the presence of a heart rate ≥ 90 (adjusted odds ratio [OR] = 3.7, 95% confidence interval [CI] = 1.4–10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95% CI = 1.2–7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 64%

Multiplex Polymerase Chain Reaction for Detection of Gastrointestinal Pathogens in Migrant Workers in Qatar

Humphrey

Ranbhise

Ibrahim

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…Se realizó un estudio observacional, descriptivo de corte transverso, muestreo no probabilístico de casos consecutivos. (13,14) incluyendo algunos parásitos intestinales.…”

Section: Materiales Y Métodosunclassified

“…Un resultado interesante en este estudio fue la presencia de más de un patógeno en 7,8% de las muestras positivas que, si bien es similar a lo obtenido por otros estudios al utilizar metodologías convencionales, es mucho menor al compararlo con lo obtenido por Filmarray, con porcentajes que van del (14,15) 30,2% al 48,1%.…”

Section: Materiales Y Métodosunclassified

Prevalence of pathogens causing acute diarrheal disease in the Asunción and Central Metropolitan area

Huber

Orrego

Ortiz

et al. 2019

Rev. salud publica Parag.

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Prevalencia de patógenos causantes de enfermedad diarreica aguda en el área Metropolitana de Asunción y Central RESUMEN Introducción: Las enfermedades diarreicas agudas (EDA) constituyen un problema de salud pública y son una de las causas más importantes de mortalidad y morbilidad en niños a nivel mundial. O b j e t i v o : D e t e r m i n a r l a p r e v a l e n c i a d e enteropatógenos causantes de EDA en el área metropolitana de Asunción y Central. Materiales y métodos: Se realizó un estudio observacional, descriptivo de corte transverso. Se analizaron 743 muestras de heces diarreicas, en las cuales se investigó la presencia de Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli diarreigénicas y Rotavirus, utilizando técnicas de referencia. Resultados: En el 31,2% (232/743) de las muestras fue posible identificar al menos uno de los patógenos e n t é r i c o s i n v e s t i g a d o s , s i e n d o l a s E. c o l i diarreigénicas fueron las bacterias identificadas con m a y o r f r e c u e n c i a , s e g u i d o p o r R o t a v i r u s , Campylobacter spp., Shigella spp. y en último lugar, Salmonella spp. Conclusión: La población más afectada corresponde a niños menores de 5 años. El principal patógeno identificado como agente causal de diarreas fueron las E. coli diarreigénicas en primer lugar, seguido por Rotavirus, Campylobacter spp., Shigella spp. y Salmonella spp. En algunas muestras se detectaron más de un patógeno entérico, encontrando incluso casos de coinfección con hasta cuatro patógenos diferentes.

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“…DEC infection prevalence has been underestimated, largely due to the lack of surveillance studies and the limited availability of molecular diagnostic techniques. Implementation of molecular techniques allowing simultaneous detection of multiple enteric pathogens, including DEC pathotypes, have shown that the prevalence of these pathotypes in diarrheal infections is higher than previously thought (Buss et al, 2015 ; Farfán et al, 2016 ).…”

Section: Introductionmentioning

confidence: 98%

Distinctive Gut Microbiota Is Associated with Diarrheagenic Escherichia coli Infections in Chilean Children

Gallardo

Izquierdo

Vidal

et al. 2017

Front. Cell. Infect. Microbiol.

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Background: Diarrheagenic Escherichia coli (DEC) strains are a major cause of diarrhea in children under 5 years of age worldwide. DEC pathogenicity relies on the interaction of bacteria with environmental factors, including the host's resident gut microbiota. Previous reports have shown changes in the gut microbiota's composition during episodes of diarrhea, which may increase the pathogenicity of DEC strains. More intense and detailed identification of microbiota strains specifically associated with DEC infections and disease is needed to pinpoint their role in DEC pathogenicity.Aim: To identify resident indicative bacterial taxa in DEC-positive diarrhea stool samples of Chilean children.Methods: We analyzed 63 diarrheal stool samples from children 1–5 years of age by FilmArray® GI in order to identify a potential pathogen and to group diarrhea episodes into those caused by DEC as sole pathogen (DEC group, 32 samples) and those caused by an enteric virus as sole pathogen (viral group, 31 samples). In addition, 30 stool samples from healthy children, negative for enteric pathogens, were evaluated (healthy group). The 16S rRNA gene was amplified and sequenced using 454 pyrosequencing. Sequences were clustered into operational taxonomic units (OTUs) at 99% identity and their representatives were used to assign them to operational phylogenetic units (OPUs) using a phylogenetic inference approach.Results: Taxa assignment using the OPU approach resulted in a lower number of units but with higher accuracy compared to the OTU approach. Data analysis indicated an increase in sequences belonging to the phylum Proteobacteria in the DEC group compared to the viral and healthy groups. Samples displayed a statistically different community structure by sample grouping by redundancy analysis and ANOVA. Escherichia albertii (p = 0.001), Citrobacter werkmanii (p = 0.001), Yersinia enterocolitica, subsp. paleartica (p = 0.048), and Haemophilus sputorum (p = 0.028) were indicative species for the DEC group as compared to the viral and healthy groups.Conclusion: Gut microbiota in Chilean children with DEC-positive diarrhea differed from microbiota associated with enteric virus and healthy children. Indicative species found in this study may prove relevant in advancing our understanding of the relationship between resident gut microbiota and DEC leading to the occurrence of disease.

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Panel Filmarray GI® en la detección de patógenos entéricos en deposiciones: experiencia preliminar

Cited by 11 publications

References 8 publications

Multiplex Polymerase Chain Reaction for Detection of Gastrointestinal Pathogens in Migrant Workers in Qatar

Multiplex Polymerase Chain Reaction for Detection of Gastrointestinal Pathogens in Migrant Workers in Qatar

Prevalence of pathogens causing acute diarrheal disease in the Asunción and Central Metropolitan area

Distinctive Gut Microbiota Is Associated with Diarrheagenic Escherichia coli Infections in Chilean Children

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