Panel strain ofKlebsiella pneumoniaefor beta-lactam antibiotic evaluation: their phenotypic and genotypic characterization

Abstract

Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.

Whole genome and transcriptome analysis reveal MALDI-TOF MS and SDS-PAGE have limited performance for the detection of the key outer membrane protein in carbapenem-resistant Klebsiella pneumoniae isolates

“…This additional band migrating behind the OmpK36 band might reflect a difference in the migration patterns of porins between strains irrespective of their molecular weight [ 11 ]. Six random K. pneumoniae isolates (YMC2011/7/B36, YMC2011/7/B774, YMC2013/6/B3993, YMC2011/8/B10311, YMC2011/11/B1440, and YMC2011/11/B7578) were selected from the panel strain bank [ 12 , 13 ] (Table 1 ). YMC2013/6/B3993 isolate lacked the OmpK35 gene due to the insertion of the IS1 transposon in the gene.…”

Combination of essential oil and ciprofloxacin to inhibit/eradicate biofilms in multidrug-resistant Klebsiella pneumoniae

“…KP are emerging as one of the primary opportunistic pathogens causing a significant rate of mortality and morbidity in hospitals and the fact that most of these organisms are MDR causes serious concern in eradication of these infections (Dsouza et al 2017). This high resistance to antibiotics is directly correlated with reduced drug penetration due to the presence of a physical barrier that is formed by the extracellular polymeric substance (EPS)-like capsule and biofilms (Abdulhasan et al 2016).…”

High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and β-Lactamase Encoding Genes in a Brazilian Intensive Care Unit

“…Klebsiella pneumoniae is a Gram-negative opportunistic bacterium that causes infections in hospitalized or otherwise immunocompromised individuals (Gorrie et al, 2017). Currently, K. pneumoniae is showing a high resistance to a broad spectrum of drugs including beta-lactam antibiotics, fluoroquinolones, and aminoglycosides (Fair and Tor, 2014; Dsouza et al, 2017). This resistance is resulting in a growing worldwide problem regarding the choice of effective antibiotic treatment for hospital-acquired infections (Davies and Davies, 2010).…”